Restriction map for
pF1KB6849
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BsiYI
BslI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstNI BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRII EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MspA1I MunI MvaI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 511 CGCG CG^CG
AcsI 426 GAATTT [GA]^AATT[CT]
Alw44I 75 GTGCAC G^TGCAC
AlwI 215 GGATC GGATC(4/5)
ApaLI 75 GTGCAC G^TGCAC
ApoI 426 GAATTT [GA]^AATT[CT]
AspHI 75 GTGCAC G[AT]GC[AT]^C
AvaII 296 GGTCC G^G[AT]CC
BbsI 3 GAAGAC GAAGAC(2/6)
BcnI 311 CCGGG CC^[GC]GG
BmyI 75 GTGCAC G[AGT]GC[ACT]^C
BpmI 219 CTCCAG CTGGAG(16/14)
BpuAI 3 GAAGAC GAAGAC(2/6)
BsiHKAI 75 GTGCAC G[AT]GC[AT]^C
BsmFI 297 GTCCC GGGAC(10/14)
Bsp1286I 75 GTGCAC G[AGT]GC[ACT]^C
BspHI 200 TCATGA T^CATGA
BspMI 101 ACCTGC ACCTGC(4/8)
BsrFI 157 ACCGGC [GA]^CCGG[CT]
BsrI 525 ACTGG ACTGG(1/-1)
BssKI 311 CCGGG ^CC[ACGT]GG
BstF5I 276 GGATG GGATG(2/0)
BstUI 511 CGCG CG^CG
BstXI 140 CCAGCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 215 GGATCT [GA]^GATC[CT]
Cfr10I 157 ACCGGC [GA]^CCGG[CT]
EcoT14I 210 CCTTGG C^C[AT][AT]GG
FokI 276 GGATG GGATG(9/13)
HaeII 39 GGCGCT [GA]GCGC^[CT]
HinfI 45 GACTC G^A[ACGT]TC
MaeII 382 ACGT A^CGT
MflI 215 GGATCT [GA]^GATC[CT]
MvnI 511 CGCG CG^CG
NciI 311 CCGGG CC^[GC]GG
PleI 45 GACTC GAGTC(4/5)
PstI 103 CTGCAG CTGCA^G
RcaI 200 TCATGA T^CATGA
ScrFI 311 CCGGG CC^[ACGT]GG
SfaNI 33 GATGC GCATC(5/9)
SfcI 103 CTGCAG C^T[GA][CT]AG
StyI 210 CCTTGG C^C[AT][AT]GG
XcmI 392 CCACTGTGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 215 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 243 CCGC CCGC(-3/-1)
410 GCGG
AlwNI 37 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
49 CAGGTTCTG
BbvI 143 GCAGC GCAGC(8/12)
270 GCAGC
BsaI 98 GAGACC GGTCTC(1/5)
480 GGTCTC
BsaJI 210 CCTTGG C^C[ACGT][ACGT]GG
311 CCGGGG
BsgI 268 GTGCAG GTGCAG(16/14)
397 GTGCAG
CfoI 40 GCGC GCG^C
510 GCGC
FauI 242 CCCGC CCCGC(4/6)
410 GCGGG
Fnu4HI 143 GCAGC GC^[ACGT]GC
270 GCAGC
HhaI 40 GCGC GCG^C
510 GCGC
HinP1I 40 GCGC G^CGC
510 GCGC
ItaI 143 GCAGC GC^[ACGT]GC
270 GCAGC
MboII 3 GAAGA GAAGA(8/7)
183 GAAGA
MslI 78 CACATGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
261 CACGGAGGTG
MwoI 273 GCAGGATGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
347 GCAAGGAAAGC
NlaIV 296 GGTCCC GG[ACGT]^[ACGT]CC
314 GGGGCC
TaqI 167 TCGA T^CGA
233 TCGA
TseI 143 GCAGC G^C[AT]GC
270 GCAGC
Tsp509I 187 AATT ^AATT
427 AATT
TthHB8I 167 TCGA T^CGA
233 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AluI BsmAI Cac8I Cfr13I DdeI
DpnII HaeIII HapII HpaII HphI
MaeIII MboI MnlI MspI NdeII
NlaIII Sau3AI Sau96I Tsp45I TspRI