Restriction map for
pF1KB6885
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BbsI BbvCI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsgI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DdeI DpnII DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI HgaI HindIII HpaI
KpnI Ksp632I MaeI MamI MboI
MfeI MflI MluI MseI MslI
MunI NaeI NcoI NdeI NdeII
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PleI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp509I Tth111I Van91I XbaI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccI 282 GTCGAC GT^[AC][GT]AC
AccII 142 CGCG CG^CG
AccIII 303 TCCGGA T^CCGGA
AcyI 508 GGCGCC G[GA]^CG[CT]C
AfaI 246 GTAC GT^AC
AflIII 278 ACGTGT A^C[GA][CT]GT
AlwNI 370 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 436 CTCGAG C^[CT]CG[GA]G
AvaII 138 GGACC G^G[AT]CC
AviII 377 TGCGCA TGC^GCA
BalI 524 TGGCCA TGG^CCA
BanI 508 GGCGCC G^G[CT][GA]CC
BanII 433 GAGCTC G[GA]GC[CT]^C
BbeI 508 GGCGCC GGCGC^C
BbrPI 418 CACGTG CAC^GTG
BcgI 23 CGATGCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 411 CCGGG CC^[GC]GG
BsaAI 418 CACGTG [CT]AC^GT[GA]
BsaHI 508 GGCGCC G[GA]^CG[CT]C
BsaI 88 GAGACC GGTCTC(1/5)
BsaWI 303 TCCGGA [AT]^CCGG[AT]
BseAI 303 TCCGGA T^CCGGA
BseRI 202 GAGGAG GAGGAG(10/8)
BsiEI 293 CGACCG CG[GA][CT]^CG
BsmBI 241 GAGACG CGTCTC(1/5)
BsoBI 436 CTCGAG C^[CT]CG[GA]G
BspEI 303 TCCGGA T^CCGGA
BsrDI 66 CATTGC GCAATG(2/0)
BssSI 57 CTCGTG CACGAG(-5/-1)
BstUI 142 CGCG CG^CG
DraII 383 GGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 524 TGGCCA [CT]^GGCC[GA]
EcoO109I 383 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 428 CCAAGG C^C[AT][AT]GG
FspI 377 TGCGCA TGC^GCA
Hin1I 508 GGCGCC G[GA]^CG[CT]C
HincII 282 GTCGAC GT[CT]^[GA]AC
HindII 282 GTCGAC GT[CT]^[GA]AC
HinfI 5 GAATC G^A[ACGT]TC
KasI 508 GGCGCC G^GCGCC
KspI 141 CCGCGG CCGC^GG
MboII 338 TCTTC GAAGA(8/7)
MluNI 524 TGGCCA TGG^CCA
MroI 303 TCCGGA T^CCGGA
MscI 524 TGGCCA TGG^CCA
MvnI 142 CGCG CG^CG
NarI 508 GGCGCC GG^CGCC
NciI 411 CCGGG CC^[GC]GG
PaeR7I 436 CTCGAG C^TCGAG
PmaCI 418 CACGTG CAC^GTG
PmlI 418 CACGTG CAC^GTG
PvuII 219 CAGCTG CAG^CTG
RsaI 246 GTAC GT^AC
SacI 433 GAGCTC GAGCT^C
SacII 141 CCGCGG CCGC^GG
SalI 282 GTCGAC G^TCGAC
SfoI 508 GGCGCC GGC^GCC
SmlI 436 CTCGAG C^T[CT][GA]AG
StyI 428 CCAAGG C^C[AT][AT]GG
TfiI 5 GAATC G^A[AT]TC
TspRI 84 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 436 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AspHI 421 GTGCTC G[AT]GC[AT]^C
433 GAGCTC
BpmI 121 CTCCAG CTGGAG(16/14)
238 CTGGAG
BsiHKAI 421 GTGCTC G[AT]GC[AT]^C
433 GAGCTC
BsmAI 88 GAGAC GTCTC(1/5)
241 GAGAC
BsrI 83 CCAGT ACTGG(1/-1)
123 CCAGT
HaeII 371 AGCGCC [GA]GCGC^[CT]
508 GGCGCC
HapII 304 CCGG C^CGG
411 CCGG
HpaII 304 CCGG C^CGG
411 CCGG
HphI 38 TCACC GGTGA(8/7)
462 TCACC
MspA1I 141 CCGCGG C[AC]G^C[GT]G
219 CAGCTG
MspI 304 CCGG C^CGG
411 CCGG
SfaNI 24 GATGC GCATC(5/9)
194 GCATC
XcmI 123 CCAGTTCGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
317 CCAGATACCCCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI BbvI BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BssKI
BstNI Cac8I CfoI Cfr13I DsaI
EcoRII FauI Fnu4HI HaeIII HhaI
HinP1I ItaI MaeII MaeIII MnlI
MvaI MwoI NlaIV Sau96I ScrFI
TaqI TseI Tsp45I TthHB8I