Restriction map for
pF1KB6858
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaII
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsiWI BsiYI
BslI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
Cfr13I ClaI CpoI DraII DraIII
DrdI DsaI Eam1105I EarI Eco47III
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HincII HindII HindIII HpaI
HphI KasI KpnI Ksp632I KspI
MaeI MaeII MamI MfeI MluI
MroI MslI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsaI RsrII SacII SalI SapI
Sau96I ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI SspBI StuI SwaI TaqI
TfiI Tth111I TthHB8I Van91I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AcsI 478 GAATTT [GA]^AATT[CT]
AcyI 554 GGCGTC G[GA]^CG[CT]C
AlwI 539 GGATC GGATC(4/5)
ApoI 478 GAATTT [GA]^AATT[CT]
AspHI 180 GAGCTC G[AT]GC[AT]^C
AvaI 19 CCCGGG C^[CT]CG[GA]G
BalI 524 TGGCCA TGG^CCA
BbvCI 426 CCTCAGC CCTCAGC(-5/-2)
BglI 38 GCCTGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 554 GGCGTC G[GA]^CG[CT]C
BseRI 31 GAGGAG GAGGAG(10/8)
BsgI 61 CTGCAC GTGCAG(16/14)
BsiEI 367 CGGCCG CG[GA][CT]^CG
BsiHKAI 180 GAGCTC G[AT]GC[AT]^C
BsmAI 122 GAGAC GTCTC(1/5)
BsoBI 19 CCCGGG C^[CT]CG[GA]G
BspHI 449 TCATGA T^CATGA
BssSI 441 CACGAG CACGAG(-5/-1)
BstF5I 23 GGATG GGATG(2/0)
BstNI 50 CCTGG CC^[AT]GG
BstYI 539 GGATCT [GA]^GATC[CT]
DpnII 540 GATC ^GATC
DraI 400 TTTAAA TTT^AAA
EagI 367 CGGCCG C^GGCCG
EclXI 367 CGGCCG C^GGCCG
Eco52I 367 CGGCCG C^GGCCG
EcoRII 50 CCTGG ^CC[AT]GG
EcoT14I 386 CCAAGG C^C[AT][AT]GG
FokI 23 GGATG GGATG(9/13)
HaeII 15 AGCGCC [GA]GCGC^[CT]
Hin1I 554 GGCGTC G[GA]^CG[CT]C
HinfI 157 GAGTC G^A[ACGT]TC
MaeIII 191 GTGAC ^GT[ACGT]AC
MboI 540 GATC ^GATC
MflI 539 GGATCT [GA]^GATC[CT]
MluNI 524 TGGCCA TGG^CCA
MscI 524 TGGCCA TGG^CCA
MseI 401 TTAA T^TAA
MvaI 50 CCTGG CC^[AT]GG
NdeII 540 GATC ^GATC
NlaIV 46 GGCTCC GG[ACGT]^[ACGT]CC
PleI 157 GAGTC GAGTC(4/5)
RcaI 449 TCATGA T^CATGA
SacI 180 GAGCTC GAGCT^C
Sau3AI 540 GATC ^GATC
SbfI 39 CCTGCAGG CCTGCA^GG
SmaI 19 CCCGGG CCC^GGG
Sse8387I 39 CCTGCAGG CCTGCA^GG
SspI 376 AATATT AAT^ATT
StyI 386 CCAAGG C^C[AT][AT]GG
Tru9I 401 TTAA T^TAA
Tsp45I 191 GTGAC ^GT[GC]AC
Tsp509I 479 AATT ^AATT
TspRI 187 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 539 GGATCT [GA]^GATC[CT]
XmaCI 19 CCCGGG C^CCGGG
XmaI 19 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Asp700I 373 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
486 GAAAGTTTTC
BanII 45 GGGCTC G[GA]GC[CT]^C
180 GAGCTC
BbvI 89 GCAGC GCAGC(8/12)
507 GCTGC
BcnI 19 CCCGG CC^[GC]GG
20 CCGGG
BmyI 45 GGGCTC G[AGT]GC[ACT]^C
180 GAGCTC
Bsp1286I 45 GGGCTC G[AGT]GC[ACT]^C
180 GAGCTC
BstXI 252 CCATAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
527 CCATCGGATTGG
CfoI 16 GCGC GCG^C
414 GCGC
Fnu4HI 89 GCAGC GC^[ACGT]GC
507 GCTGC
HapII 20 CCGG C^CGG
370 CCGG
HhaI 16 GCGC GCG^C
414 GCGC
HinP1I 16 GCGC G^CGC
414 GCGC
HpaII 20 CCGG C^CGG
370 CCGG
ItaI 89 GCAGC GC^[ACGT]GC
507 GCTGC
MspI 20 CCGG C^CGG
370 CCGG
NciI 19 CCCGG CC^[GC]GG
20 CCGGG
PstI 40 CTGCAG CTGCA^G
473 CTGCAG
SfaNI 24 GATGC GCATC(5/9)
323 GCATC
SfcI 40 CTGCAG C^T[GA][CT]AG
473 CTGCAG
TseI 89 GCAGC G^C[AT]GC
507 GCTGC
XmnI 373 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
486 GAAAGTTTTC
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# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AluI BsaJI BssKI Cac8I DdeI
EaeI HaeIII HgaI MboII MnlI
MwoI NlaIII ScrFI