Restriction map for
pF1KB6824
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AclI AcsI
AcyI AfaI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AviII AvrII BalI
BamHI BanI BbeI BbsI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsiEI BsiWI BsiYI BslI
BsmAI BsmBI Bsp1407I BspDI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MscI MseI MspA1I MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspV PacI PaeR7I
PflMI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp509I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 95 CGCG CG^CG
AccIII 554 TCCGGA T^CCGGA
AciI 226 GCGG CCGC(-3/-1)
AflIII 278 ACATGT A^C[GA][CT]GT
Asp700I 201 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 448 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 308 CCCGAG C^[CT]CG[GA]G
AvaII 378 GGACC G^G[AT]CC
BbrPI 157 CACGTG CAC^GTG
BbvCI 537 CCTCAGC CCTCAGC(-5/-2)
BfaI 37 CTAG C^TAG
BglII 338 AGATCT A^GATCT
BpmI 382 CTGGAG CTGGAG(16/14)
BsaAI 157 CACGTG [CT]AC^GT[GA]
BsaWI 554 TCCGGA [AT]^CCGG[AT]
BseAI 554 TCCGGA T^CCGGA
BsgI 54 CTGCAC GTGCAG(16/14)
BsmFI 124 GTCCC GGGAC(10/14)
BsmI 90 GCATTC GAATGC(1/-1)
BsoBI 308 CCCGAG C^[CT]CG[GA]G
BspEI 554 TCCGGA T^CCGGA
BspLU11I 278 ACATGT A^CATGT
BstAPI 322 GCAAACTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 95 CGCG CG^CG
BstYI 338 AGATCT [GA]^GATC[CT]
EcoT14I 521 CCAAGG C^C[AT][AT]GG
HgaI 390 GACGC GACGC(5/10)
MaeI 37 CTAG C^TAG
MaeII 158 ACGT A^CGT
MaeIII 479 GTGAC ^GT[ACGT]AC
MflI 338 AGATCT [GA]^GATC[CT]
MroI 554 TCCGGA T^CCGGA
MslI 177 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 95 CGCG CG^CG
NruI 94 TCGCGA TCG^CGA
NspI 278 ACATGT [GA]CATG^[CT]
PflFI 448 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 298 GAGTC GAGTC(4/5)
PmaCI 157 CACGTG CAC^GTG
PmlI 157 CACGTG CAC^GTG
PpuMI 377 AGGACCT [GA]G^G[AT]CC[CT]
SfaNI 183 GATGC GCATC(5/9)
SfcI 135 CTACAG C^T[GA][CT]AG
StyI 521 CCAAGG C^C[AT][AT]GG
TaqI 506 TCGA T^CGA
Tsp45I 479 GTGAC ^GT[GC]AC
TspRI 354 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 448 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 506 TCGA T^CGA
XhoII 338 AGATCT [GA]^GATC[CT]
XmnI 201 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 218 GGATC GGATC(4/5)
557 GGATC
BbvI 33 GCAGC GCAGC(8/12)
87 GCTGC
BcnI 195 CCGGG CC^[GC]GG
398 CCCGG
BsaJI 236 CCGAGG C^C[ACGT][ACGT]GG
521 CCAAGG
BstNI 48 CCAGG CC^[AT]GG
381 CCTGG
DdeI 407 CTAAG C^T[ACGT]AG
538 CTCAG
DraII 377 AGGACCT [GA]G^G[ACGT]CC[CT]
524 AGGCCCC
EarI 28 GAAGAG CTCTTC(1/4)
141 GAAGAG
EcoO109I 377 AGGACCT [GA]G^G[ACGT]CC[CT]
524 AGGCCCC
EcoRII 48 CCAGG ^CC[AT]GG
381 CCTGG
Ksp632I 28 GAAGAG CTCTTC(1/4)
141 GAAGAG
MvaI 48 CCAGG CC^[AT]GG
381 CCTGG
NciI 195 CCGGG CC^[GC]GG
398 CCCGG
SacI 190 GAGCTC GAGCT^C
313 GAGCTC
SapI 28 GAAGAGC GCTCTTC(1/4)
141 GAAGAGC
SmlI 82 CTCAAG C^T[CT][GA]AG
316 CTCAAG
TfiI 98 GATTC G^A[AT]TC
201 GAATC
TseI 33 GCAGC G^C[AT]GC
87 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI AspHI BanII BmyI BseRI
BsiHKAI Bsp1286I BssKI BstF5I Cac8I
Cfr13I DpnII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MboI MboII MnlI MspI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI