Restriction map for
pF1KB6847
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI AluI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstNI
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI DsaI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRII EcoRV EcoT14I
EcoT22I FbaI FokI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MamI MfeI MluI
MluNI MroI MscI MunI MvaI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StyI SwaI
TaqI Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 184 GTCTAC GT^[AC][GT]AC
AflIII 275 ACATGT A^C[GA][CT]GT
AlwI 236 GGATC GGATC(4/5)
AlwNI 308 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 225 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 359 GAGCCC G[GA]GC[CT]^C
BbsI 168 GTCTTC GAAGAC(2/6)
BpuAI 168 GTCTTC GAAGAC(2/6)
BsaJI 8 CCTCGG C^C[ACGT][ACGT]GG
BseRI 388 GAGGAG GAGGAG(10/8)
BsiEI 415 CGGCCG CG[GA][CT]^CG
BsmAI 332 GAGAC GTCTC(1/5)
BspHI 377 TCATGA T^CATGA
BspLU11I 275 ACATGT A^CATGT
BspMI 195 GCAGGT ACCTGC(4/8)
BsrBI 261 GAGCGG CCGCTC(-3/-3)
BsrI 321 CCAGT ACTGG(1/-1)
BstYI 236 GGATCT [GA]^GATC[CT]
DdeI 146 CTCAG C^T[ACGT]AG
DraIII 318 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 415 CGGCCG C^GGCCG
EclXI 415 CGGCCG C^GGCCG
Eco52I 415 CGGCCG C^GGCCG
HaeII 310 GGCGCT [GA]GCGC^[CT]
MaeIII 316 GTCAC ^GT[ACGT]AC
MflI 236 GGATCT [GA]^GATC[CT]
MseI 151 TTAA T^TAA
MslI 378 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 457 CCGCTG C[AC]G^C[GT]G
RcaI 377 TCATGA T^CATGA
SphI 86 GCATGC GCATG^C
StuI 5 AGGCCT AGG^CCT
TfiI 92 GAATC G^A[AT]TC
Tru9I 151 TTAA T^TAA
Tsp45I 316 GTCAC ^GT[GC]AC
TspRI 322 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 236 GGATCT [GA]^GATC[CT]
XmnI 225 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccII 352 CGCG CG^CG
396 CGCG
AvaI 9 CTCGGG C^[CT]CG[GA]G
356 CCCGAG
AvaII 294 GGACC G^G[AT]CC
471 GGTCC
BsoBI 9 CTCGGG C^[CT]CG[GA]G
356 CCCGAG
BsrFI 413 GCCGGC [GA]^CCGG[CT]
417 GCCGGC
BstUI 352 CGCG CG^CG
396 CGCG
Cfr10I 413 GCCGGC [GA]^CCGG[CT]
417 GCCGGC
DraII 293 GGGACCT [GA]G^G[ACGT]CC[CT]
470 GGGTCCT
EaeI 398 CGGCCA [CT]^GGCC[GA]
415 CGGCCG
EcoO109I 293 GGGACCT [GA]G^G[ACGT]CC[CT]
470 GGGTCCT
MvnI 352 CGCG CG^CG
396 CGCG
MwoI 404 GCAAGTGCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
477 GCGCCGTCAGC
NaeI 413 GCCGGC GCC^GGC
417 GCCGGC
NgoMIV 413 GCCGGC G^CCGGC
417 GCCGGC
NspI 86 GCATGC [GA]CATG^[CT]
275 ACATGT
PleI 253 GACTC GAGTC(4/5)
439 GACTC
PpuMI 293 GGGACCT [GA]G^G[AT]CC[CT]
470 GGGTCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AfaI BanI BbvI BcnI
BmyI BsiYI BslI BsmFI Bsp1286I
BssKI Cac8I CfoI Cfr13I DpnII
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MboI MboII MnlI MspI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI TseI