Restriction map for
pF1KB6871
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1286I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MseI MspA1I MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AhdI 511 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 32 GGATC GGATC(4/5)
AspEI 511 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 297 GGTCC G^G[AT]CC
BbrPI 453 CACGTG CAC^GTG
BcnI 288 CCGGG CC^[GC]GG
BglI 225 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 319 CTCCAG CTGGAG(16/14)
BsaAI 453 CACGTG [CT]AC^GT[GA]
BsaJI 483 CCAAGG C^C[ACGT][ACGT]GG
Bsp1407I 101 TGTACA T^GTACA
BsrBI 292 GAGCGG CCGCTC(-3/-3)
BsrGI 101 TGTACA T^GTACA
BsrI 512 ACTGG ACTGG(1/-1)
BssSI 523 CACGAG CACGAG(-5/-1)
BstAPI 381 GCAGGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 32 GGATCT [GA]^GATC[CT]
DdeI 254 CTCAG C^T[ACGT]AG
DpnII 33 GATC ^GATC
DraII 257 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 511 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 257 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 483 CCAAGG C^C[AT][AT]GG
HapII 288 CCGG C^CGG
HpaII 288 CCGG C^CGG
MaeIII 127 GTCAC ^GT[ACGT]AC
MboI 33 GATC ^GATC
MflI 32 GGATCT [GA]^GATC[CT]
MspI 288 CCGG C^CGG
NciI 288 CCGGG CC^[GC]GG
NdeII 33 GATC ^GATC
NlaIV 296 GGGTCC GG[ACGT]^[ACGT]CC
NspI 386 ACATGC [GA]CATG^[CT]
PmaCI 453 CACGTG CAC^GTG
PmlI 453 CACGTG CAC^GTG
PstI 331 CTGCAG CTGCA^G
Sau3AI 33 GATC ^GATC
SspBI 101 TGTACA T^GTACA
StyI 483 CCAAGG C^C[AT][AT]GG
Tsp45I 127 GTCAC ^GT[GC]AC
XhoII 32 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 18 CGCG CG^CG
29 CGCG
AfaI 102 GTAC GT^AC
498 GTAC
BsiYI 139 CCAACCATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
288 CCGGGAGCGGG
BslI 139 CCAACCATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
288 CCGGGAGCGGG
BsmFI 197 GGGAC GGGAC(10/14)
519 GTCCC
BstNI 210 CCAGG CC^[AT]GG
465 CCAGG
BstUI 18 CGCG CG^CG
29 CGCG
Cac8I 82 GCTGGC GC[ACGT]^[ACGT]GC
410 GCGAGC
CfoI 19 GCGC GCG^C
537 GCGC
EcoRII 210 CCAGG ^CC[AT]GG
465 CCAGG
FauI 294 GCGGG CCCGC(4/6)
348 GCGGG
HhaI 19 GCGC GCG^C
537 GCGC
HinP1I 19 GCGC G^CGC
537 GCGC
HphI 8 TCACC GGTGA(8/7)
128 TCACC
MaeII 200 ACGT A^CGT
454 ACGT
MslI 264 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
399 CATGCCCGTG
MvaI 210 CCAGG CC^[AT]GG
465 CCAGG
MvnI 18 CGCG CG^CG
29 CGCG
RsaI 102 GTAC GT^AC
498 GTAC
SfcI 331 CTGCAG C^T[GA][CT]AG
433 CTACAG
TspRI 164 CAGTG CA[GC]TG[ACGT][ACGT]^
356 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI AluI BbvI BssKI BstF5I
Cfr13I Fnu4HI FokI HaeIII HinfI
ItaI MboII MnlI MwoI NlaIII
Sau96I ScrFI SfaNI TfiI TseI