Restriction map for
pF1KB6895
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MfeI MflI MluI
MluNI MroI MscI MslI MspA1I
MunI MwoI NaeI NarI NgoMIV
NheI NlaIV NotI NsiI NspI
PacI PaeR7I PflFI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tsp45I TspRI Tth111I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 175 GTAGAC GT^[AC][GT]AC
AccII 27 CGCG CG^CG
AciI 4 CCGC CCGC(-3/-1)
AfaI 328 GTAC GT^AC
AgeI 41 ACCGGT A^CCGGT
AseI 232 ATTAAT AT^TAAT
AsnI 232 ATTAAT AT^TAAT
BbsI 534 GTCTTC GAAGAC(2/6)
BpuAI 534 GTCTTC GAAGAC(2/6)
BsaBI 24 GATCGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 41 ACCGGT [AT]^CCGG[AT]
BsmFI 15 GTCCC GGGAC(10/14)
BsmI 423 GAATGC GAATGC(1/-1)
BspMI 365 ACCTGC ACCTGC(4/8)
BsrFI 41 ACCGGT [GA]^CCGG[CT]
BstBI 479 TTCGAA TT^CGAA
BstF5I 471 GGATG GGATG(2/0)
BstNI 33 CCTGG CC^[AT]GG
BstUI 27 CGCG CG^CG
Cfr10I 41 ACCGGT [GA]^CCGG[CT]
DdeI 212 CTCAG C^T[ACGT]AG
DsaI 297 CCATGG C^C[GA][CT]GG
EcoRII 33 CCTGG ^CC[AT]GG
EcoT14I 297 CCATGG C^C[AT][AT]GG
FokI 471 GGATG GGATG(9/13)
HaeIII 85 GGCC GG^CC
HindIII 404 AAGCTT A^AGCTT
MaeIII 259 GTTAC ^GT[ACGT]AC
MamI 24 GATCGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvaI 33 CCTGG CC^[AT]GG
MvnI 27 CGCG CG^CG
NcoI 297 CCATGG C^CATGG
NdeI 370 CATATG CA^TATG
NruI 26 TCGCGA TCG^CGA
NspV 479 TTCGAA TT^CGAA
PflMI 87 CCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 41 ACCGGT A^CCGGT
PleI 97 GACTC GAGTC(4/5)
PshBI 232 ATTAAT AT^TAAT
RsaI 328 GTAC GT^AC
SfuI 479 TTCGAA TT^CGAA
SspI 197 AATATT AAT^ATT
StyI 297 CCATGG C^C[AT][AT]GG
TaqI 480 TCGA T^CGA
TthHB8I 480 TCGA T^CGA
Van91I 87 CCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 12 enzymes) #
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AlwI 30 GATCC GGATC(4/5)
106 GATCC
BbvI 427 GCAGC GCAGC(8/12)
517 GCAGC
BclI 137 TGATCA T^GATCA
383 TGATCA
BcnI 17 CCCGG CC^[GC]GG
185 CCGGG
BsaJI 33 CCTGGG C^C[ACGT][ACGT]GG
297 CCATGG
FbaI 137 TGATCA T^GATCA
383 TGATCA
HphI 381 GGTGA GGTGA(8/7)
546 GGTGA
MseI 233 TTAA T^TAA
287 TTAA
NciI 17 CCCGG CC^[GC]GG
185 CCGGG
NlaIII 298 CATG CATG^
539 CATG
Tru9I 233 TTAA T^TAA
287 TTAA
TseI 427 GCAGC G^C[AT]GC
517 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AluI BsiYI BslI BssKI DpnII
Fnu4HI HapII HinfI HpaII ItaI
MboI MboII MnlI MspI NdeII
Sau3AI ScrFI TfiI Tsp509I