Restriction map for
pF1KB6896
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# NO CUT ENZYMES ( 171 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BcgI BciVI
BclI BfaI BfrI BlnI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI HphI
KasI KpnI Ksp632I KspI MaeI
MaeIII MamI MfeI MluI MluNI
MroI MscI MseI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccII 409 CGCG CG^CG
AvaII 534 GGACC G^G[AT]CC
BanII 592 GAGCCC G[GA]GC[CT]^C
BbvCI 551 CCTCAGC CCTCAGC(-5/-2)
BcnI 531 CCGGG CC^[GC]GG
BglI 331 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 347 AGATCT A^GATCT
BlpI 207 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 207 GCTGAGC GC^T[ACGT]AGC
BsaAI 476 CACGTA [CT]AC^GT[GA]
BsaI 403 GAGACC GGTCTC(1/5)
BsmAI 403 GAGAC GTCTC(1/5)
BsmFI 533 GGGAC GGGAC(10/14)
BstF5I 422 GGATG GGATG(2/0)
BstUI 409 CGCG CG^CG
BstXI 29 CCACCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 207 GCTGAGC GC^T[ACGT]AGC
EaeI 14 TGGCCG [CT]^GGCC[GA]
FauI 407 CCCGC CCCGC(4/6)
FokI 422 GGATG GGATG(9/13)
HapII 531 CCGG C^CGG
HincII 388 GTCAAC GT[CT]^[GA]AC
HindII 388 GTCAAC GT[CT]^[GA]AC
HpaII 531 CCGG C^CGG
MspA1I 576 CAGCTG C[AC]G^C[GT]G
MspI 531 CCGG C^CGG
MvnI 409 CGCG CG^CG
NciI 531 CCGGG CC^[GC]GG
NspI 448 ACATGC [GA]CATG^[CT]
PpuMI 533 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 576 CAGCTG CAG^CTG
ScaI 112 AGTACT AGT^ACT
SfaNI 457 GATGC GCATC(5/9)
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 113 GTAC GT^AC
479 GTAC
AflIII 61 ACACGT A^C[GA][CT]GT
475 ACACGT
AlwI 309 GATCC GGATC(4/5)
71 GGATC
BbsI 249 GAAGAC GAAGAC(2/6)
471 GAAGAC
BmyI 338 GGGCAC G[AGT]GC[ACT]^C
592 GAGCCC
BpuAI 249 GAAGAC GAAGAC(2/6)
471 GAAGAC
BsaJI 11 CCCTGG C^C[ACGT][ACGT]GG
335 CCAGGG
Bsp1286I 338 GGGCAC G[AGT]GC[ACT]^C
592 GAGCCC
BsrI 106 ACTGG ACTGG(1/-1)
115 ACTGG
Cfr13I 375 GGCCC G^G[ACGT]CC
534 GGACC
DraII 374 AGGCCCC [GA]G^G[ACGT]CC[CT]
533 GGGACCT
EcoO109I 374 AGGCCCC [GA]G^G[ACGT]CC[CT]
533 GGGACCT
HinfI 190 GAGTC G^A[ACGT]TC
496 GACTC
MaeII 63 ACGT A^CGT
477 ACGT
NlaIII 400 CATG CATG^
449 CATG
NlaIV 375 GGCCCC GG[ACGT]^[ACGT]CC
533 GGGACC
PleI 496 GACTC GAGTC(4/5)
190 GAGTC
PstI 430 CTGCAG CTGCA^G
579 CTGCAG
RsaI 113 GTAC GT^AC
479 GTAC
Sau96I 375 GGCCC G^G[ACGT]CC
534 GGACC
SfcI 430 CTGCAG C^T[GA][CT]AG
579 CTGCAG
TaqI 125 TCGA T^CGA
197 TCGA
TspRI 261 CAGTG CA[GC]TG[ACGT][ACGT]^
369 CAGTG
TthHB8I 125 TCGA T^CGA
197 TCGA
XcmI 26 CCACCACCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
575 CCAGCTGCAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI BbvI BpmI BseRI
BsiYI BslI BspMI BssKI BstNI
BstYI Cac8I DdeI DpnII EcoRII
Fnu4HI HaeIII ItaI MboI MboII
MflI MnlI MvaI MwoI NdeII
Sau3AI ScrFI TseI XhoII