################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbsI BcgI BciVI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HgaI HincII HindII HindIII HinfI HpaI HphI KpnI Ksp632I KspI MaeI MamI MboII MfeI MflI MluI MluNI MroI MscI MseI MunI NcoI NheI NotI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 64 GACGTC GACGT^C AfaI 207 GTAC GT^AC AlwI 576 GATCC GGATC(4/5) ApaI 390 GGGCCC GGGCC^C AvaI 326 CCCGGG C^[CT]CG[GA]G AvaII 201 GGACC G^G[AT]CC BanI 46 GGCGCC G^G[CT][GA]CC BanII 390 GGGCCC G[GA]GC[CT]^C BbeI 46 GGCGCC GGCGC^C BbvCI 337 GCTGAGG CCTCAGC(-5/-2) BclI 252 TGATCA T^GATCA BglI 137 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 390 GGGCCC G[AGT]GC[ACT]^C BsmFI 329 GGGAC GGGAC(10/14) BsoBI 326 CCCGGG C^[CT]CG[GA]G Bsp1286I 390 GGGCCC G[AGT]GC[ACT]^C BspMI 273 GCAGGT ACCTGC(4/8) BsrBI 24 GAGCGG CCGCTC(-3/-3) BsrFI 43 GCCGGC [GA]^CCGG[CT] BstAPI 291 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 613 CCAACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 43 GCCGGC [GA]^CCGG[CT] DsaI 397 CCACGG C^C[GA][CT]GG FauI 451 GCGGG CCCGC(4/6) FbaI 252 TGATCA T^GATCA HaeII 46 GGCGCC [GA]GCGC^[CT] KasI 46 GGCGCC G^GCGCC MslI 147 CATTGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 43 GCCGGC GCC^GGC NarI 46 GGCGCC GG^CGCC NdeI 237 CATATG CA^TATG NgoMIV 43 GCCGGC G^CCGGC NruI 306 TCGCGA TCG^CGA PstI 244 CTGCAG CTGCA^G PvuII 295 CAGCTG CAG^CTG RsaI 207 GTAC GT^AC SfcI 244 CTGCAG C^T[GA][CT]AG SfoI 46 GGCGCC GGC^GCC SmaI 326 CCCGGG CCC^GGG XcmI 138 CCAAAAAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 326 CCCGGG C^CCGGG XmaI 326 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AccII 307 CGCG CG^CG 450 CGCG AcyI 46 GGCGCC G[GA]^CG[CT]C 64 GACGTC AlwNI 70 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 511 CAGTCACTG BsaHI 46 GGCGCC G[GA]^CG[CT]C 64 GACGTC BsrI 369 ACTGG ACTGG(1/-1) 510 CCAGT BstF5I 152 GGATG GGATG(2/0) 465 GGATG BstUI 307 CGCG CG^CG 450 CGCG DdeI 338 CTGAG C^T[ACGT]AG 601 CTTAG FokI 152 GGATG GGATG(9/13) 465 GGATG Hin1I 46 GGCGCC G[GA]^CG[CT]C 64 GACGTC MspA1I 163 CCGCTG C[AC]G^C[GT]G 295 CAGCTG MvnI 307 CGCG CG^CG 450 CGCG NlaIII 333 CATG CATG^ 357 CATG ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AluI BbvI BcnI BsaJI BseRI BsgI BsiYI BslI BssKI BstNI Cac8I CfoI Cfr13I DpnII DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MaeIII MboI MnlI MspI MvaI MwoI NciI NdeII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I TspRI