##################################
# NO CUT ENZYMES ( 161 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BbrPI BbsI BcgI BciVI
BfaI BfrI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FseI FspI HgaI HincII HindII
HindIII HinfI HpaI HphI KpnI
Ksp632I KspI MaeI MamI MboII
MfeI MflI MluI MluNI MroI
MscI MseI MunI NcoI NheI
NotI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TfiI Tru9I Tsp509I Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 64 GACGTC GACGT^C
AfaI 207 GTAC GT^AC
AlwI 576 GATCC GGATC(4/5)
ApaI 390 GGGCCC GGGCC^C
AvaI 326 CCCGGG C^[CT]CG[GA]G
AvaII 201 GGACC G^G[AT]CC
BanI 46 GGCGCC G^G[CT][GA]CC
BanII 390 GGGCCC G[GA]GC[CT]^C
BbeI 46 GGCGCC GGCGC^C
BbvCI 337 GCTGAGG CCTCAGC(-5/-2)
BclI 252 TGATCA T^GATCA
BglI 137 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 390 GGGCCC G[AGT]GC[ACT]^C
BsmFI 329 GGGAC GGGAC(10/14)
BsoBI 326 CCCGGG C^[CT]CG[GA]G
Bsp1286I 390 GGGCCC G[AGT]GC[ACT]^C
BspMI 273 GCAGGT ACCTGC(4/8)
BsrBI 24 GAGCGG CCGCTC(-3/-3)
BsrFI 43 GCCGGC [GA]^CCGG[CT]
BstAPI 291 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 613 CCAACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 43 GCCGGC [GA]^CCGG[CT]
DsaI 397 CCACGG C^C[GA][CT]GG
FauI 451 GCGGG CCCGC(4/6)
FbaI 252 TGATCA T^GATCA
HaeII 46 GGCGCC [GA]GCGC^[CT]
KasI 46 GGCGCC G^GCGCC
MslI 147 CATTGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 43 GCCGGC GCC^GGC
NarI 46 GGCGCC GG^CGCC
NdeI 237 CATATG CA^TATG
NgoMIV 43 GCCGGC G^CCGGC
NruI 306 TCGCGA TCG^CGA
PstI 244 CTGCAG CTGCA^G
PvuII 295 CAGCTG CAG^CTG
RsaI 207 GTAC GT^AC
SfcI 244 CTGCAG C^T[GA][CT]AG
SfoI 46 GGCGCC GGC^GCC
SmaI 326 CCCGGG CCC^GGG
XcmI 138 CCAAAAAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 326 CCCGGG C^CCGGG
XmaI 326 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AccII 307 CGCG CG^CG
450 CGCG
AcyI 46 GGCGCC G[GA]^CG[CT]C
64 GACGTC
AlwNI 70 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
511 CAGTCACTG
BsaHI 46 GGCGCC G[GA]^CG[CT]C
64 GACGTC
BsrI 369 ACTGG ACTGG(1/-1)
510 CCAGT
BstF5I 152 GGATG GGATG(2/0)
465 GGATG
BstUI 307 CGCG CG^CG
450 CGCG
DdeI 338 CTGAG C^T[ACGT]AG
601 CTTAG
FokI 152 GGATG GGATG(9/13)
465 GGATG
Hin1I 46 GGCGCC G[GA]^CG[CT]C
64 GACGTC
MspA1I 163 CCGCTG C[AC]G^C[GT]G
295 CAGCTG
MvnI 307 CGCG CG^CG
450 CGCG
NlaIII 333 CATG CATG^
357 CATG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI BbvI BcnI BsaJI
BseRI BsgI BsiYI BslI BssKI
BstNI Cac8I CfoI Cfr13I DpnII
DraII EcoO109I EcoRII Fnu4HI HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeII MaeIII MboI MnlI MspI
MvaI MwoI NciI NdeII NlaIV
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp45I TspRI