Restriction map for
pF1KB6905
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# NO CUT ENZYMES ( 171 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BclI BglI BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr13I ClaI CpoI
DraI DraII DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KpnI MaeII
MfeI MluI MluNI MroI MscI
MslI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SalI SapI Sau96I SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 6 CGCG CG^CG
AcsI 529 AAATTT [GA]^AATT[CT]
AfaI 42 GTAC GT^AC
AflII 445 CTTAAG C^TTAAG
ApoI 529 AAATTT [GA]^AATT[CT]
BciVI 201 GGATAC GTATCC(6/5)
BfaI 190 CTAG C^TAG
BfrI 445 CTTAAG C^TTAAG
BglII 387 AGATCT A^GATCT
BsaJI 5 CCGCGG C^C[ACGT][ACGT]GG
BsiEI 8 CGGCCG CG[GA][CT]^CG
BsmFI 20 GGGAC GGGAC(10/14)
BssSI 49 CTCGTG CACGAG(-5/-1)
BstNI 33 CCAGG CC^[AT]GG
BstUI 6 CGCG CG^CG
BstYI 387 AGATCT [GA]^GATC[CT]
CfoI 85 GCGC GCG^C
DdeI 334 CTCAG C^T[ACGT]AG
DsaI 5 CCGCGG C^C[GA][CT]GG
EagI 8 CGGCCG C^GGCCG
EarI 217 GAAGAG CTCTTC(1/4)
EclXI 8 CGGCCG C^GGCCG
Eco52I 8 CGGCCG C^GGCCG
EcoRII 33 CCAGG ^CC[AT]GG
HaeII 84 GGCGCT [GA]GCGC^[CT]
HhaI 85 GCGC GCG^C
HinP1I 85 GCGC G^CGC
HindIII 497 AAGCTT A^AGCTT
Ksp632I 217 GAAGAG CTCTTC(1/4)
KspI 5 CCGCGG CCGC^GG
MaeI 190 CTAG C^TAG
MflI 387 AGATCT [GA]^GATC[CT]
MspA1I 5 CCGCGG C[AC]G^C[GT]G
MvaI 33 CCAGG CC^[AT]GG
MvnI 6 CGCG CG^CG
NaeI 10 GCCGGC GCC^GGC
NgoMIV 10 GCCGGC G^CCGGC
RsaI 42 GTAC GT^AC
SacII 5 CCGCGG CCGC^GG
SfaNI 466 GCATC GCATC(5/9)
SmlI 445 CTTAAG C^T[CT][GA]AG
Tsp45I 283 GTCAC ^GT[GC]AC
TspRI 160 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 387 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AluI 443 AGCT AG^CT
498 AGCT
BcnI 185 CCCGG CC^[GC]GG
516 CCGGG
BsaBI 124 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
388 GATCTGGATC
BsiYI 11 CCGGCTGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
45 CCGGCTCGTGG
BslI 11 CCGGCTGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
45 CCGGCTCGTGG
BsrFI 10 GCCGGC [GA]^CCGG[CT]
44 ACCGGC
Cac8I 10 GCCGGC GC[ACGT]^[ACGT]GC
14 GCTGGC
Cfr10I 10 GCCGGC [GA]^CCGG[CT]
44 ACCGGC
FauI 18 GCGGG CCCGC(4/6)
62 GCGGG
HinfI 145 GATTC G^A[ACGT]TC
330 GATTC
HphI 89 TCACC GGTGA(8/7)
556 TCACC
MamI 124 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
388 GATCTGGATC
MseI 370 TTAA T^TAA
446 TTAA
MwoI 62 GCGGGGGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
68 GCGGCGTGGGC
NciI 185 CCCGG CC^[GC]GG
516 CCGGG
NlaIV 25 GGCTCC GG[ACGT]^[ACGT]CC
226 GGAGCC
TfiI 145 GATTC G^A[AT]TC
330 GATTC
Tru9I 370 TTAA T^TAA
446 TTAA
Tsp509I 372 AATT ^AATT
530 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AlwI BbvI BsrI BssKI
BstF5I DpnII EaeI Fnu4HI FokI
HaeIII HapII HpaII ItaI MaeIII
MboI MboII MnlI MspI NdeII
NlaIII Sau3AI ScrFI TseI