Restriction map for
pF1KE0850
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwNI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BciVI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaI HpaII KasI KpnI
KspI MaeI MaeII MfeI MflI
MluI MroI MspA1I MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PleI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tth111I XbaI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 28 GAATTT [GA]^AATT[CT]
Alw44I 658 GTGCAC G^TGCAC
AlwI 249 GATCC GGATC(4/5)
AocI 695 CCTCAGG CC^T[ACGT]AGG
ApaLI 658 GTGCAC G^TGCAC
ApoI 28 GAATTT [GA]^AATT[CT]
Asp700I 708 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 478 CTCGAG C^[CT]CG[GA]G
BbvCI 678 CCTCAGC CCTCAGC(-5/-2)
BbvI 14 GCAGC GCAGC(8/12)
BcgI 470 GCATCTTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 461 TGATCA T^GATCA
BsaBI 243 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 478 CTCGAG C^[CT]CG[GA]G
Bsp1407I 833 TGTACA T^GTACA
BsrDI 74 CATTGC GCAATG(2/0)
BsrGI 833 TGTACA T^GTACA
BstAPI 595 GCAATCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 695 CCTCAGG CC^T[ACGT]AGG
Cac8I 930 GCTGGC GC[ACGT]^[ACGT]GC
DraIII 253 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 268 GACCACATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 88 CTCTTC CTCTTC(1/4)
Eco81I 695 CCTCAGG CC^T[ACGT]AGG
FbaI 461 TGATCA T^GATCA
Fnu4HI 14 GCAGC GC^[ACGT]GC
HindIII 528 AAGCTT A^AGCTT
ItaI 14 GCAGC GC^[ACGT]GC
Ksp632I 88 CTCTTC CTCTTC(1/4)
MamI 243 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 114 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 478 CTCGAG C^TCGAG
PflMI 252 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 572 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 738 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 54 GAGCTC GAGCT^C
SfcI 102 CTACAG C^T[GA][CT]AG
SmlI 478 CTCGAG C^T[CT][GA]AG
SspBI 833 TGTACA T^GTACA
TaqI 479 TCGA T^CGA
TseI 14 GCAGC G^C[AT]GC
TspRI 642 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 479 TCGA T^CGA
Van91I 252 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 789 CCACAGCACACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 478 CTCGAG C^TCGAG
XmnI 708 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AciI 507 CCGC CCGC(-3/-1)
879 CCGC
AvaII 267 GGACC G^G[AT]CC
573 GGACC
BalI 116 TGGCCA TGG^CCA
668 TGGCCA
BanII 54 GAGCTC G[GA]GC[CT]^C
499 GAGCCC
BbsI 708 GAAGAC GAAGAC(2/6)
313 GTCTTC
BpuAI 708 GAAGAC GAAGAC(2/6)
313 GTCTTC
BseRI 690 CTCCTC GAGGAG(10/8)
693 CTCCTC
DraII 542 AGGCCCC [GA]G^G[ACGT]CC[CT]
572 GGGACCC
EcoO109I 542 AGGCCCC [GA]G^G[ACGT]CC[CT]
572 GGGACCC
MaeIII 22 GTGAC ^GT[ACGT]AC
646 GTCAC
MluNI 116 TGGCCA TGG^CCA
668 TGGCCA
MscI 116 TGGCCA TGG^CCA
668 TGGCCA
MseI 392 TTAA T^TAA
893 TTAA
Tru9I 392 TTAA T^TAA
893 TTAA
Tsp45I 22 GTGAC ^GT[GC]AC
646 GTCAC
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AfaI AluI AspHI BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BssSI BstF5I BstNI
Cfr13I DdeI DpnII DsaI EaeI
EcoRII EcoT14I FokI HaeIII HphI
MboI MboII MnlI MvaI MwoI
NcoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
Tsp509I