Restriction map for
pF1KB8020
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII AccI AcyI AflII AflIII
AgeI AhdI Alw44I AocI ApaLI
AscI AseI AsnI AspEI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsiEI BsiWI
BsmI BspDI BspHI BspLU11I BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraII EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI Hin1I
HindIII HpaI KasI KspI MamI
MluI MspA1I NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI PvuII RcaI RsrII
SacI SacII SalI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI SspI StuI SwaI XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 514 GGTACC G^GTACC
AccIII 1197 TCCGGA T^CCGGA
AclI 374 AACGTT AA^CGTT
AlwNI 554 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 640 AGCGCT AGC^GCT
ApaI 490 GGGCCC GGGCC^C
Asp700I 240 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 514 GGTACC G^GTACC
AspHI 968 GAGCAC G[AT]GC[AT]^C
AspI 139 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 896 CCCGAG C^[CT]CG[GA]G
BanI 514 GGTACC G^G[CT][GA]CC
BanII 490 GGGCCC G[GA]GC[CT]^C
BlpI 1140 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1140 GCTAAGC GC^T[ACGT]AGC
BsaI 839 GAGACC GGTCTC(1/5)
BseAI 1197 TCCGGA T^CCGGA
BsgI 1994 CTGCAC GTGCAG(16/14)
BsiHKAI 968 GAGCAC G[AT]GC[AT]^C
BsoBI 896 CCCGAG C^[CT]CG[GA]G
Bsp1407I 110 TGTACA T^GTACA
BspEI 1197 TCCGGA T^CCGGA
BspMI 511 GCAGGT ACCTGC(4/8)
BsrBI 565 GAGCGG CCGCTC(-3/-3)
BsrGI 110 TGTACA T^GTACA
CelII 1140 GCTAAGC GC^T[ACGT]AGC
DraIII 188 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 576 GACATCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 640 AGCGCT AGC^GCT
KpnI 514 GGTACC GGTAC^C
MaeII 375 ACGT A^CGT
MfeI 1250 CAATTG C^AATTG
MroI 1197 TCCGGA T^CCGGA
MunI 1250 CAATTG C^AATTG
NcoI 1509 CCATGG C^CATGG
NspI 1387 ACATGC [GA]CATG^[CT]
PflFI 139 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1504 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 374 AACGTT AA^CGTT
SapI 1021 GCTCTTC GCTCTTC(1/4)
SbfI 508 CCTGCAGG CCTGCA^GG
ScaI 1708 AGTACT AGT^ACT
Sse8387I 508 CCTGCAGG CCTGCA^GG
SspBI 110 TGTACA T^GTACA
TaqI 945 TCGA T^CGA
Tth111I 139 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 945 TCGA T^CGA
Van91I 1504 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 240 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 1629 CGCG CG^CG
1692 CGCG
AcsI 704 AAATTC [GA]^AATT[CT]
1242 GAATTT
AlwI 505 GATCC GGATC(4/5)
1368 GGATC
ApoI 704 AAATTC [GA]^AATT[CT]
1242 GAATTT
BalI 78 TGGCCA TGG^CCA
1163 TGGCCA
BsaWI 260 ACCGGA [AT]^CCGG[AT]
1197 TCCGGA
BsmBI 584 CGTCTC CGTCTC(1/5)
818 GAGACG
BssSI 998 CACGAG CACGAG(-5/-1)
1523 CACGAG
BstUI 1629 CGCG CG^CG
1692 CGCG
BstYI 504 AGATCC [GA]^GATC[CT]
1368 GGATCT
DsaI 1509 CCATGG C^C[GA][CT]GG
1976 CCGTGG
EcoT14I 1434 CCTTGG C^C[AT][AT]GG
1509 CCATGG
HaeII 640 AGCGCT [GA]GCGC^[CT]
953 GGCGCT
HapII 261 CCGG C^CGG
1198 CCGG
HgaI 583 GCGTC GACGC(5/10)
1630 GCGTC
HincII 856 GTCAAC GT[CT]^[GA]AC
1971 GTCAAC
HindII 856 GTCAAC GT[CT]^[GA]AC
1971 GTCAAC
HpaII 261 CCGG C^CGG
1198 CCGG
MflI 504 AGATCC [GA]^GATC[CT]
1368 GGATCT
MluNI 78 TGGCCA TGG^CCA
1163 TGGCCA
MscI 78 TGGCCA TGG^CCA
1163 TGGCCA
MspI 261 CCGG C^CGG
1198 CCGG
MvnI 1629 CGCG CG^CG
1692 CGCG
PleI 1036 GACTC GAGTC(4/5)
1683 GACTC
PstI 172 CTGCAG CTGCA^G
509 CTGCAG
SfaNI 1732 GATGC GCATC(5/9)
930 GCATC
SfcI 172 CTGCAG C^T[GA][CT]AG
509 CTGCAG
SmlI 464 CTCAAG C^T[CT][GA]AG
1657 CTTGAG
SpeI 454 ACTAGT A^CTAGT
1998 ACTAGT
StyI 1434 CCTTGG C^C[AT][AT]GG
1509 CCATGG
XhoII 504 AGATCC [GA]^GATC[CT]
1368 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BfaI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EarI EcoRII Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI