################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## Acc65I AccI AccII AciI AclI AcsI AflII AflIII AgeI AhdI AluI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BlnI BlpI BpmI Bpu1102I BsaAI BsaI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI BspDI BspLU11I BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MfeI MluI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tsp509I Tth111I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 175 GACGTC GACGT^C AccIII 477 TCCGGA T^CCGGA AcyI 175 GACGTC G[GA]^CG[CT]C BanI 418 GGCACC G^G[CT][GA]CC BbsI 342 GAAGAC GAAGAC(2/6) BfaI 370 CTAG C^TAG BglI 28 GCCGTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 122 AGATCT A^GATCT BpuAI 342 GAAGAC GAAGAC(2/6) BsaBI 475 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 175 GACGTC G[GA]^CG[CT]C BsaWI 477 TCCGGA [AT]^CCGG[AT] BseAI 477 TCCGGA T^CCGGA BsiYI 38 CCATGAAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 38 CCATGAAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 453 GTCCC GGGAC(10/14) BsmI 516 GAATGC GAATGC(1/-1) Bsp1407I 146 TGTACA T^GTACA BspEI 477 TCCGGA T^CCGGA BspHI 260 TCATGA T^CATGA BspMI 421 ACCTGC ACCTGC(4/8) BsrDI 324 CATTGC GCAATG(2/0) BsrFI 140 ACCGGC [GA]^CCGG[CT] BsrGI 146 TGTACA T^GTACA BsrI 319 CCAGT ACTGG(1/-1) BstF5I 531 GGATG GGATG(2/0) Cac8I 165 GCTCGC GC[ACGT]^[ACGT]GC Cfr10I 140 ACCGGC [GA]^CCGG[CT] DdeI 314 CTGAG C^T[ACGT]AG DrdI 403 GACTTTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FokI 531 GGATG GGATG(9/13) Hin1I 175 GACGTC G[GA]^CG[CT]C MaeI 370 CTAG C^TAG MaeII 176 ACGT A^CGT MamI 475 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 477 TCCGGA T^CCGGA MseI 350 TTAA T^TAA PstI 68 CTGCAG CTGCA^G RcaI 260 TCATGA T^CATGA SfaNI 532 GATGC GCATC(5/9) SfcI 68 CTGCAG C^T[GA][CT]AG SfiI 27 GGCCGTCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 199 CTCAAG C^T[CT][GA]AG SspBI 146 TGTACA T^GTACA TaqI 569 TCGA T^CGA Tru9I 350 TTAA T^TAA TthHB8I 569 TCGA T^CGA XbaI 369 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 147 GTAC GT^AC 438 GTAC AvaII 336 GGACC G^G[AT]CC 525 GGACC BamHI 86 GGATCC G^GATCC 474 GGATCC BanII 163 GGGCTC G[GA]GC[CT]^C 463 GAGCCC BbvI 67 GCTGC GCAGC(8/12) 383 GCTGC BmyI 163 GGGCTC G[AGT]GC[ACT]^C 463 GAGCCC Bsp1286I 163 GGGCTC G[AGT]GC[ACT]^C 463 GAGCCC BstEII 102 GGTGACC G^GT[ACGT]ACC 136 GGTGACC BstNI 90 CCAGG CC^[AT]GG 590 CCAGG BstPI 102 GGTGACC G^GT[ACGT]ACC 136 GGTGACC EcoO65I 102 GGTGACC G^GT[ACGT]ACC 136 GGTGACC EcoRII 90 CCAGG ^CC[AT]GG 590 CCAGG EcoT14I 131 CCATGG C^C[AT][AT]GG 389 CCATGG Fnu4HI 67 GCTGC GC^[ACGT]GC 383 GCTGC HaeII 77 GGCGCT [GA]GCGC^[CT] 194 AGCGCC HinfI 451 GAGTC G^A[ACGT]TC 551 GAGTC ItaI 67 GCTGC GC^[ACGT]GC 383 GCTGC MslI 33 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 261 CATGAGCATG MvaI 90 CCAGG CC^[AT]GG 590 CCAGG NcoI 131 CCATGG C^CATGG 389 CCATGG PleI 451 GAGTC GAGTC(4/5) 551 GAGTC RsaI 147 GTAC GT^AC 438 GTAC StyI 131 CCATGG C^C[AT][AT]GG 389 CCATGG TseI 67 GCTGC G^C[AT]GC 383 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AlwI BalI BcnI BsaJI BssKI BstYI CfoI Cfr13I DpnII DsaI EaeI HaeIII HapII HhaI HinP1I HpaII HphI MaeIII MboI MboII MflI MluNI MnlI MscI MspI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI Tsp45I TspRI XhoII