Restriction map for pF1KB6911
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

Acc65I      AccI        AccII       AciI        AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
AluI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfrI        BlnI        BlpI        BpmI        Bpu1102I    
BsaAI       BsaI        BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsoBI       
BspDI       BspLU11I    BsrBI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraII       DraIII      EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HgaI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MfeI        MluI        
MspA1I      MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        TfiI        Tsp509I     Tth111I     Van91I      
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII         175  GACGTC               GACGT^C
AccIII        477  TCCGGA               T^CCGGA
AcyI          175  GACGTC               G[GA]^CG[CT]C
BanI          418  GGCACC               G^G[CT][GA]CC
BbsI          342  GAAGAC               GAAGAC(2/6)
BfaI          370  CTAG                 C^TAG
BglI           28  GCCGTCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         122  AGATCT               A^GATCT
BpuAI         342  GAAGAC               GAAGAC(2/6)
BsaBI         475  GATCCGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         175  GACGTC               G[GA]^CG[CT]C
BsaWI         477  TCCGGA               [AT]^CCGG[AT]
BseAI         477  TCCGGA               T^CCGGA
BsiYI          38  CCATGAAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           38  CCATGAAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         453  GTCCC                GGGAC(10/14)
BsmI          516  GAATGC               GAATGC(1/-1)
Bsp1407I      146  TGTACA               T^GTACA
BspEI         477  TCCGGA               T^CCGGA
BspHI         260  TCATGA               T^CATGA
BspMI         421  ACCTGC               ACCTGC(4/8)
BsrDI         324  CATTGC               GCAATG(2/0)
BsrFI         140  ACCGGC               [GA]^CCGG[CT]
BsrGI         146  TGTACA               T^GTACA
BsrI          319  CCAGT                ACTGG(1/-1)
BstF5I        531  GGATG                GGATG(2/0)
Cac8I         165  GCTCGC               GC[ACGT]^[ACGT]GC
Cfr10I        140  ACCGGC               [GA]^CCGG[CT]
DdeI          314  CTGAG                C^T[ACGT]AG
DrdI          403  GACTTTGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FokI          531  GGATG                GGATG(9/13)
Hin1I         175  GACGTC               G[GA]^CG[CT]C
MaeI          370  CTAG                 C^TAG
MaeII         176  ACGT                 A^CGT
MamI          475  GATCCGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          477  TCCGGA               T^CCGGA
MseI          350  TTAA                 T^TAA
PstI           68  CTGCAG               CTGCA^G
RcaI          260  TCATGA               T^CATGA
SfaNI         532  GATGC                GCATC(5/9)
SfcI           68  CTGCAG               C^T[GA][CT]AG
SfiI           27  GGCCGTCATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI          199  CTCAAG               C^T[CT][GA]AG
SspBI         146  TGTACA               T^GTACA
TaqI          569  TCGA                 T^CGA
Tru9I         350  TTAA                 T^TAA
TthHB8I       569  TCGA                 T^CGA
XbaI          369  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AfaI          147  GTAC                 GT^AC
              438  GTAC                
AvaII         336  GGACC                G^G[AT]CC
              525  GGACC               
BamHI          86  GGATCC               G^GATCC
              474  GGATCC              
BanII         163  GGGCTC               G[GA]GC[CT]^C
              463  GAGCCC              
BbvI           67  GCTGC                GCAGC(8/12)
              383  GCTGC               
BmyI          163  GGGCTC               G[AGT]GC[ACT]^C
              463  GAGCCC              
Bsp1286I      163  GGGCTC               G[AGT]GC[ACT]^C
              463  GAGCCC              
BstEII        102  GGTGACC              G^GT[ACGT]ACC
              136  GGTGACC             
BstNI          90  CCAGG                CC^[AT]GG
              590  CCAGG               
BstPI         102  GGTGACC              G^GT[ACGT]ACC
              136  GGTGACC             
EcoO65I       102  GGTGACC              G^GT[ACGT]ACC
              136  GGTGACC             
EcoRII         90  CCAGG                ^CC[AT]GG
              590  CCAGG               
EcoT14I       131  CCATGG               C^C[AT][AT]GG
              389  CCATGG              
Fnu4HI         67  GCTGC                GC^[ACGT]GC
              383  GCTGC               
HaeII          77  GGCGCT               [GA]GCGC^[CT]
              194  AGCGCC              
HinfI         451  GAGTC                G^A[ACGT]TC
              551  GAGTC               
ItaI           67  GCTGC                GC^[ACGT]GC
              383  GCTGC               
MslI           33  CATGGCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              261  CATGAGCATG          
MvaI           90  CCAGG                CC^[AT]GG
              590  CCAGG               
NcoI          131  CCATGG               C^CATGG
              389  CCATGG              
PleI          451  GAGTC                GAGTC(4/5)
              551  GAGTC               
RsaI          147  GTAC                 GT^AC
              438  GTAC                
StyI          131  CCATGG               C^C[AT][AT]GG
              389  CCATGG              
TseI           67  GCTGC                G^C[AT]GC
              383  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AlwI        BalI        BcnI        BsaJI       BssKI       
BstYI       CfoI        Cfr13I      DpnII       DsaI        
EaeI        HaeIII      HapII       HhaI        HinP1I      
HpaII       HphI        MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MspI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       Tsp45I      TspRI       
XhoII       
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