Restriction map for
pF1KB6913
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstF5I BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FauI FbaI FokI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI KasI KpnI KspI MaeII
MaeIII MamI MfeI MluI MluNI
MroI MscI MseI MunI MvnI
NarI NciI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tru9I Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AcsI 349 GAATTC [GA]^AATT[CT]
AfaI 291 GTAC GT^AC
AlwI 275 GGATC GGATC(4/5)
ApoI 349 GAATTC [GA]^AATT[CT]
Asp700I 462 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 600 GGTCC G^G[AT]CC
BglII 338 AGATCT A^GATCT
BsiYI 542 CCAGCTGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 542 CCAGCTGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspHI 204 TCATGA T^CATGA
BsrFI 24 GCCGGC [GA]^CCGG[CT]
BsrI 186 ACTGG ACTGG(1/-1)
BstAPI 583 GCAGACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 338 AGATCT [GA]^GATC[CT]
Cfr10I 24 GCCGGC [GA]^CCGG[CT]
EcoRI 349 GAATTC G^AATTC
EcoT14I 326 CCAAGG C^C[AT][AT]GG
HincII 532 GTCAAC GT[CT]^[GA]AC
HindII 532 GTCAAC GT[CT]^[GA]AC
HindIII 230 AAGCTT A^AGCTT
MflI 338 AGATCT [GA]^GATC[CT]
MslI 205 CATGAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 24 GCCGGC GCC^GGC
NgoMIV 24 GCCGGC G^CCGGC
NspI 419 ACATGC [GA]CATG^[CT]
RcaI 204 TCATGA T^CATGA
RsaI 291 GTAC GT^AC
SspI 262 AATATT AAT^ATT
StyI 326 CCAAGG C^C[AT][AT]GG
TaqI 513 TCGA T^CGA
TthHB8I 513 TCGA T^CGA
XhoII 338 AGATCT [GA]^GATC[CT]
XmnI 462 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
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AciI 495 CCGC CCGC(-3/-1)
498 CCGC
BbvI 220 GCAGC GCAGC(8/12)
78 GCTGC
BfaI 67 CTAG C^TAG
424 CTAG
BseRI 39 GAGGAG GAGGAG(10/8)
256 GAGGAG
BsmAI 406 GAGAC GTCTC(1/5)
510 GTCTC
BsmFI 323 GTCCC GGGAC(10/14)
601 GTCCC
BspMI 439 ACCTGC ACCTGC(4/8)
16 GCAGGT
Cfr13I 453 GGCCC G^G[ACGT]CC
600 GGTCC
DpnII 276 GATC ^GATC
339 GATC
EarI 286 GAAGAG CTCTTC(1/4)
403 GAAGAG
HaeIII 330 GGCC GG^CC
453 GGCC
HapII 25 CCGG C^CGG
414 CCGG
HpaII 25 CCGG C^CGG
414 CCGG
HphI 378 GGTGA GGTGA(8/7)
307 TCACC
Ksp632I 286 GAAGAG CTCTTC(1/4)
403 GAAGAG
MaeI 67 CTAG C^TAG
424 CTAG
MboI 276 GATC ^GATC
339 GATC
MspA1I 279 CAGCTG C[AC]G^C[GT]G
543 CAGCTG
MspI 25 CCGG C^CGG
414 CCGG
MwoI 223 GCTTTCAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
583 GCAGACTGTGC
NdeII 276 GATC ^GATC
339 GATC
NlaIII 205 CATG CATG^
420 CATG
NlaIV 568 GGAGCC GG[ACGT]^[ACGT]CC
600 GGTCCC
PvuII 279 CAGCTG CAG^CTG
543 CAGCTG
Sau3AI 276 GATC ^GATC
339 GATC
Sau96I 453 GGCCC G^G[ACGT]CC
600 GGTCC
SmlI 36 CTTGAG C^T[CT][GA]AG
505 CTCAAG
TseI 78 GCTGC G^C[AT]GC
220 GCAGC
Tsp509I 106 AATT ^AATT
350 AATT
TspRI 185 CACTG CA[GC]TG[ACGT][ACGT]^
607 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI BsaJI BssKI BstNI Cac8I
DdeI EcoRII Fnu4HI HinfI ItaI
MboII MnlI MvaI ScrFI TfiI