Restriction map for
pF1KB6850
##################################
# NO CUT ENZYMES ( 188 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DdeI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoT22I FauI
FbaI Fnu4HI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
ItaI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI TaqI TseI
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AciI 126 CCGC CCGC(-3/-1)
AluI 57 AGCT AG^CT
Alw44I 103 GTGCAC G^TGCAC
AlwNI 515 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 103 GTGCAC G^TGCAC
BamHI 396 GGATCC G^GATCC
BanI 51 GGTGCC G^G[CT][GA]CC
BciVI 173 GTATCC GTATCC(6/5)
BglII 489 AGATCT A^GATCT
BpmI 512 CTCCAG CTGGAG(16/14)
BseRI 145 GAGGAG GAGGAG(10/8)
Bsp1407I 111 TGTACA T^GTACA
BsrGI 111 TGTACA T^GTACA
BssKI 229 CCAGG ^CC[ACGT]GG
BstNI 229 CCAGG CC^[AT]GG
Cac8I 54 GCCAGC GC[ACGT]^[ACGT]GC
DraI 264 TTTAAA TTT^AAA
EcoNI 42 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 229 CCAGG ^CC[AT]GG
HphI 272 TCACC GGTGA(8/7)
MvaI 229 CCAGG CC^[AT]GG
MwoI 315 GCAACCACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 24 CATG CATG^
PflMI 93 CCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 340 GACTC GAGTC(4/5)
ScrFI 229 CCAGG CC^[ACGT]GG
SmlI 496 CTTGAG C^T[CT][GA]AG
SspBI 111 TGTACA T^GTACA
SspI 199 AATATT AAT^ATT
StuI 231 AGGCCT AGG^CCT
TfiI 565 GAATC G^A[AT]TC
Van91I 93 CCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 397 GATCC GGATC(4/5)
396 GGATC
AspHI 103 GTGCAC G[AT]GC[AT]^C
290 GAGCAC
BfaI 235 CTAG C^TAG
481 CTAG
BmyI 103 GTGCAC G[AGT]GC[ACT]^C
290 GAGCAC
BsiHKAI 103 GTGCAC G[AT]GC[AT]^C
290 GAGCAC
Bsp1286I 103 GTGCAC G[AGT]GC[ACT]^C
290 GAGCAC
BstF5I 295 CATCC GGATG(2/0)
3 GGATG
BstYI 396 GGATCC [GA]^GATC[CT]
489 AGATCT
DpnII 397 GATC ^GATC
490 GATC
EcoRV 413 GATATC GAT^ATC
468 GATATC
EcoT14I 47 CCAAGG C^C[AT][AT]GG
392 CCAAGG
FokI 295 CATCC GGATG(9/13)
3 GGATG
HaeIII 232 GGCC GG^CC
503 GGCC
HinfI 340 GACTC G^A[ACGT]TC
565 GAATC
MaeI 235 CTAG C^TAG
481 CTAG
MboI 397 GATC ^GATC
490 GATC
MflI 396 GGATCC [GA]^GATC[CT]
489 AGATCT
MseI 265 TTAA T^TAA
382 TTAA
NdeII 397 GATC ^GATC
490 GATC
NlaIV 51 GGTGCC GG[ACGT]^[ACGT]CC
396 GGATCC
Sau3AI 397 GATC ^GATC
490 GATC
SfaNI 208 GATGC GCATC(5/9)
508 GATGC
StyI 47 CCAAGG C^C[AT][AT]GG
392 CCAAGG
Tru9I 265 TTAA T^TAA
382 TTAA
Tsp509I 95 AATT ^AATT
183 AATT
XhoII 396 GGATCC [GA]^GATC[CT]
489 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AfaI BsaJI BsiYI BslI BsrI
MaeIII MboII MnlI RsaI SfcI
Tsp45I TspRI