Restriction map for
pF1KB6919
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI AspEI
AspI AvaI AviII AvrII BalI
BbeI BbrPI BbvCI BcgI BclI
BfrI BglI BlnI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmFI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I ClaI
CpoI DraI DraII DraIII DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI KasI KspI MamI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspI StuI StyI SwaI
TaqI TfiI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 53 GGTACC G^GTACC
AlwNI 17 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 48 CCTGAGG CC^T[ACGT]AGG
Asp700I 585 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 53 GGTACC G^GTACC
AspHI 337 GAGCTC G[AT]GC[AT]^C
AvaII 225 GGACC G^G[AT]CC
BamHI 41 GGATCC G^GATCC
BanI 53 GGTACC G^G[CT][GA]CC
BanII 337 GAGCTC G[GA]GC[CT]^C
BbsI 144 GAAGAC GAAGAC(2/6)
BciVI 568 GGATAC GTATCC(6/5)
BglII 206 AGATCT A^GATCT
BlpI 334 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 334 GCTGAGC GC^T[ACGT]AGC
BpuAI 144 GAAGAC GAAGAC(2/6)
BsaJI 86 CCCAGG C^C[ACGT][ACGT]GG
BsgI 298 GTGCAG GTGCAG(16/14)
BsiHKAI 337 GAGCTC G[AT]GC[AT]^C
BsmAI 613 GAGAC GTCTC(1/5)
BsmBI 613 GAGACG CGTCTC(1/5)
BsmI 406 GAATGC GAATGC(1/-1)
Bsp1407I 485 TGTACA T^GTACA
BsrFI 71 ACCGGC [GA]^CCGG[CT]
BsrGI 485 TGTACA T^GTACA
Bsu36I 48 CCTGAGG CC^T[ACGT]AGG
CelII 334 GCTGAGC GC^T[ACGT]AGC
Cfr10I 71 ACCGGC [GA]^CCGG[CT]
DrdI 253 GACCTTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 48 CCTGAGG CC^T[ACGT]AGG
HgaI 147 GACGC GACGC(5/10)
HinfI 462 GACTC G^A[ACGT]TC
KpnI 53 GGTACC GGTAC^C
MaeII 632 ACGT A^CGT
PleI 462 GACTC GAGTC(4/5)
PstI 58 CTGCAG CTGCA^G
PvuII 157 CAGCTG CAG^CTG
SacI 337 GAGCTC GAGCT^C
SbfI 57 CCTGCAGG CCTGCA^GG
ScaI 503 AGTACT AGT^ACT
SmlI 340 CTCAAG C^T[CT][GA]AG
Sse8387I 57 CCTGCAGG CCTGCA^GG
SspBI 485 TGTACA T^GTACA
Tsp509I 347 AATT ^AATT
TspRI 288 CACTG CA[GC]TG[ACGT][ACGT]^
XmnI 585 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
BcnI 12 CCGGG CC^[GC]GG
38 CCGGG
BfaI 265 CTAG C^TAG
353 CTAG
BmyI 337 GAGCTC G[AGT]GC[ACT]^C
356 GTGCCC
BpmI 464 CTCCAG CTGGAG(16/14)
151 CTGGAG
BsiYI 105 CCTACAGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
321 CCGCTACATGG
BslI 105 CCTACAGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
321 CCGCTACATGG
Bsp1286I 337 GAGCTC G[AGT]GC[ACT]^C
356 GTGCCC
BspMI 56 ACCTGC ACCTGC(4/8)
480 GCAGGT
BsrI 123 ACTGG ACTGG(1/-1)
228 CCAGT
BstAPI 16 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
300 GCAGAGCTTGC
BstNI 87 CCAGG CC^[AT]GG
390 CCAGG
BstYI 41 GGATCC [GA]^GATC[CT]
206 AGATCT
CfoI 163 GCGC GCG^C
410 GCGC
EcoRII 87 CCAGG ^CC[AT]GG
390 CCAGG
FauI 28 GCGGG CCCGC(4/6)
111 GCGGG
HhaI 163 GCGC GCG^C
410 GCGC
HinP1I 163 GCGC G^CGC
410 GCGC
HphI 141 GGTGA GGTGA(8/7)
240 GGTGA
MaeI 265 CTAG C^TAG
353 CTAG
MaeIII 460 GTGAC ^GT[ACGT]AC
634 GTCAC
MflI 41 GGATCC [GA]^GATC[CT]
206 AGATCT
MseI 397 TTAA T^TAA
440 TTAA
MspA1I 109 CAGCGG C[AC]G^C[GT]G
157 CAGCTG
MvaI 87 CCAGG CC^[AT]GG
390 CCAGG
NciI 12 CCGGG CC^[GC]GG
38 CCGGG
Tru9I 397 TTAA T^TAA
440 TTAA
Tsp45I 460 GTGAC ^GT[GC]AC
634 GTCAC
XhoII 41 GGATCC [GA]^GATC[CT]
206 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BssKI BstF5I Cac8I Cfr13I DdeI
DpnII EarI Fnu4HI FokI HaeIII
HapII HpaII ItaI Ksp632I MboI
MboII MnlI MspI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TseI