Restriction map for
pF1KB6920
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BpmI BpuAI BsaAI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HphI KasI KpnI KspI MaeII
MfeI MflI MluI MroI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcsI 169 GAATTT [GA]^AATT[CT]
ApoI 169 GAATTT [GA]^AATT[CT]
AspHI 287 GTGCTC G[AT]GC[AT]^C
AvaII 405 GGACC G^G[AT]CC
BalI 632 TGGCCA TGG^CCA
BfaI 101 CTAG C^TAG
BlpI 143 GCTCAGC GC^T[ACGT]AGC
BmyI 287 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 143 GCTCAGC GC^T[ACGT]AGC
BsaBI 462 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 221 GGTCTC GGTCTC(1/5)
BsiHKAI 287 GTGCTC G[AT]GC[AT]^C
BsmAI 222 GTCTC GTCTC(1/5)
BsmFI 576 GTCCC GGGAC(10/14)
BsmI 297 GAATGC GAATGC(1/-1)
Bsp1286I 287 GTGCTC G[AGT]GC[ACT]^C
BspMI 214 GCAGGT ACCTGC(4/8)
BstBI 342 TTCGAA TT^CGAA
CelII 143 GCTCAGC GC^T[ACGT]AGC
Cfr13I 405 GGACC G^G[ACGT]CC
DpnII 360 GATC ^GATC
HinfI 462 GATTC G^A[ACGT]TC
MaeI 101 CTAG C^TAG
MamI 462 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 360 GATC ^GATC
MluNI 632 TGGCCA TGG^CCA
MscI 632 TGGCCA TGG^CCA
MseI 652 TTAA T^TAA
NdeII 360 GATC ^GATC
NspI 139 GCATGC [GA]CATG^[CT]
NspV 342 TTCGAA TT^CGAA
PvuII 146 CAGCTG CAG^CTG
Sau3AI 360 GATC ^GATC
Sau96I 405 GGACC G^G[ACGT]CC
SfuI 342 TTCGAA TT^CGAA
SphI 139 GCATGC GCATG^C
TaqI 343 TCGA T^CGA
TfiI 462 GATTC G^A[AT]TC
Tru9I 652 TTAA T^TAA
TthHB8I 343 TCGA T^CGA
XcmI 635 CCAAAGGTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 320 GTAC GT^AC
477 GTAC
BcnI 252 CCGGG CC^[GC]GG
432 CCGGG
BsrDI 128 CATTGC GCAATG(2/0)
424 GCAATG
Cac8I 139 GCATGC GC[ACGT]^[ACGT]GC
247 GCTGGC
EarI 227 CTCTTC CTCTTC(1/4)
58 GAAGAG
EcoT14I 443 CCAAGG C^C[AT][AT]GG
579 CCAAGG
FauI 47 GCGGG CCCGC(4/6)
383 GCGGG
HapII 252 CCGG C^CGG
432 CCGG
HpaII 252 CCGG C^CGG
432 CCGG
Ksp632I 227 CTCTTC CTCTTC(1/4)
58 GAAGAG
MspA1I 45 CAGCGG C[AC]G^C[GT]G
146 CAGCTG
MspI 252 CCGG C^CGG
432 CCGG
MwoI 139 GCATGCTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
238 GCGGCTTCTGC
NciI 252 CCGGG CC^[GC]GG
432 CCGGG
RsaI 320 GTAC GT^AC
477 GTAC
SfcI 42 CTACAG C^T[GA][CT]AG
419 CTACAG
SmlI 290 CTCAAG C^T[CT][GA]AG
658 CTCAAG
StyI 443 CCAAGG C^C[AT][AT]GG
579 CCAAGG
Tsp509I 162 AATT ^AATT
170 AATT
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AluI BbvI BsaJI BseRI
BsiYI BslI BsrI BssKI BstF5I
BstNI DdeI EaeI EcoRII Fnu4HI
FokI HaeIII ItaI MaeIII MboII
MnlI MvaI NlaIII ScrFI SfaNI
TseI TspRI