################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AflII AgeI AhdI Alw44I ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CelII ClaI CpoI DraI DraIII DsaI EagI Eam1105I EarI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccIII 181 TCCGGA T^CCGGA AcyI 130 GGCGTC G[GA]^CG[CT]C AfaI 507 GTAC GT^AC AflIII 142 ACATGT A^C[GA][CT]GT AocI 92 CCTCAGG CC^T[ACGT]AGG Aor51HI 659 AGCGCT AGC^GCT BanII 616 GAGCCC G[GA]GC[CT]^C BbsI 481 GAAGAC GAAGAC(2/6) BclI 425 TGATCA T^GATCA BfaI 436 CTAG C^TAG BglI 562 GCCTTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 260 AGATCT A^GATCT BpuAI 481 GAAGAC GAAGAC(2/6) BsaHI 130 GGCGTC G[GA]^CG[CT]C BsaWI 181 TCCGGA [AT]^CCGG[AT] BseAI 181 TCCGGA T^CCGGA BsiYI 494 CCTTGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 494 CCTTGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspEI 181 TCCGGA T^CCGGA BspHI 399 TCATGA T^CATGA BspLU11I 142 ACATGT A^CATGT BsrFI 33 GCCGGT [GA]^CCGG[CT] BsrI 541 ACTGG ACTGG(1/-1) BstAPI 69 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 92 CCTCAGG CC^T[ACGT]AGG Cfr10I 33 GCCGGT [GA]^CCGG[CT] DrdI 124 GACACAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 659 AGCGCT AGC^GCT Eco81I 92 CCTCAGG CC^T[ACGT]AGG EcoNI 494 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 56 CCCGC CCCGC(4/6) FbaI 425 TGATCA T^GATCA Hin1I 130 GGCGTC G[GA]^CG[CT]C MaeI 436 CTAG C^TAG MaeII 419 ACGT A^CGT MroI 181 TCCGGA T^CCGGA NspI 142 ACATGT [GA]CATG^[CT] PleI 646 GAGTC GAGTC(4/5) PstI 256 CTGCAG CTGCA^G PvuII 666 CAGCTG CAG^CTG RcaI 399 TCATGA T^CATGA RsaI 507 GTAC GT^AC SfcI 256 CTGCAG C^T[GA][CT]AG StuI 326 AGGCCT AGG^CCT TaqI 368 TCGA T^CGA TfiI 396 GATTC G^A[AT]TC Tsp509I 158 AATT ^AATT TspRI 390 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 368 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### BalI 497 TGGCCA TGG^CCA 569 TGGCCA BcnI 594 CCGGG CC^[GC]GG 599 CCGGG BmyI 88 GTGCCC G[AGT]GC[ACT]^C 616 GAGCCC BpmI 663 CTCCAG CTGGAG(16/14) 523 CTGGAG BsaI 490 GAGACC GGTCTC(1/5) 526 GAGACC Bsp1286I 88 GTGCCC G[AGT]GC[ACT]^C 616 GAGCCC BstF5I 237 GGATG GGATG(2/0) 612 GGATG BstYI 260 AGATCT [GA]^GATC[CT] 629 AGATCC Cac8I 55 GCCCGC GC[ACGT]^[ACGT]GC 86 GCGTGC DraII 169 GGGGCCT [GA]G^G[ACGT]CC[CT] 379 AGGGCCT EaeI 497 TGGCCA [CT]^GGCC[GA] 569 TGGCCA EcoO109I 169 GGGGCCT [GA]G^G[ACGT]CC[CT] 379 AGGGCCT EcoT14I 494 CCTTGG C^C[AT][AT]GG 578 CCAAGG FokI 237 GGATG GGATG(9/13) 612 GGATG HaeII 532 AGCGCC [GA]GCGC^[CT] 659 AGCGCT HgaI 131 GCGTC GACGC(5/10) 296 GCGTC HinfI 396 GATTC G^A[ACGT]TC 646 GAGTC MaeIII 229 GTGAC ^GT[ACGT]AC 298 GTCAC MflI 260 AGATCT [GA]^GATC[CT] 629 AGATCC MluNI 497 TGGCCA TGG^CCA 569 TGGCCA MscI 497 TGGCCA TGG^CCA 569 TGGCCA MslI 400 CATGAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 546 CATGAATGTG MspA1I 272 CCGCTG C[AC]G^C[GT]G 666 CAGCTG NciI 594 CCGGG CC^[GC]GG 599 CCGGG StyI 494 CCTTGG C^C[AT][AT]GG 578 CCAAGG Tsp45I 229 GTGAC ^GT[GC]AC 298 GTCAC XhoII 260 AGATCT [GA]^GATC[CT] 629 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AciI AluI AlwI AlwNI BbvI BsaJI BsmAI BsmFI BssKI BstNI CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI