Restriction map for
pF1KB6927
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AviII
BalI BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DraI
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MseI MslI MunI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp45I Tsp509I Tth111I Van91I XbaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccII 524 CGCG CG^CG
AcyI 578 GGCGCC G[GA]^CG[CT]C
AflIII 38 ACGTGT A^C[GA][CT]GT
AspHI 55 GAGCTC G[AT]GC[AT]^C
AvrII 306 CCTAGG C^CTAGG
BanI 578 GGCGCC G^G[CT][GA]CC
BbeI 578 GGCGCC GGCGC^C
BfaI 307 CTAG C^TAG
BlnI 306 CCTAGG C^CTAGG
BsaHI 578 GGCGCC G[GA]^CG[CT]C
BsiHKAI 55 GAGCTC G[AT]GC[AT]^C
BsiYI 489 CCAGGGCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 489 CCAGGGCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 612 GTCTC GTCTC(1/5)
BstAPI 96 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 524 CGCG CG^CG
BstXI 272 CCAGGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 203 AGATCC [GA]^GATC[CT]
DsaI 390 CCATGG C^C[GA][CT]GG
Hin1I 578 GGCGCC G[GA]^CG[CT]C
HinfI 610 GAGTC G^A[ACGT]TC
KasI 578 GGCGCC G^GCGCC
MaeI 307 CTAG C^TAG
MaeII 38 ACGT A^CGT
MaeIII 515 GTTAC ^GT[ACGT]AC
MboII 558 GAAGA GAAGA(8/7)
MflI 203 AGATCC [GA]^GATC[CT]
MvnI 524 CGCG CG^CG
NaeI 575 GCCGGC GCC^GGC
NarI 578 GGCGCC GG^CGCC
NcoI 390 CCATGG C^CATGG
NgoMIV 575 GCCGGC G^CCGGC
PleI 610 GAGTC GAGTC(4/5)
SacI 55 GAGCTC GAGCT^C
SfoI 578 GGCGCC GGC^GCC
SgrAI 574 CGCCGGCG C[GA]^CCGG[CT]G
StuI 396 AGGCCT AGG^CCT
TspRI 233 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 224 CCAAGGCCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 203 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 204 GATCC GGATC(4/5)
411 GGATC
AlwNI 97 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
233 CAGTGGCTG
BseRI 58 CTCCTC GAGGAG(10/8)
535 GAGGAG
BsmFI 35 GGGAC GGGAC(10/14)
42 GTCCC
BsrBI 31 GAGCGG CCGCTC(-3/-3)
64 GAGCGG
BsrFI 190 ACCGGC [GA]^CCGG[CT]
575 GCCGGC
Cfr10I 190 ACCGGC [GA]^CCGG[CT]
575 GCCGGC
DpnII 204 GATC ^GATC
412 GATC
DraII 107 AGGCCCT [GA]G^G[ACGT]CC[CT]
302 AGGGCCT
EcoO109I 107 AGGCCCT [GA]G^G[ACGT]CC[CT]
302 AGGGCCT
HaeII 196 AGCGCC [GA]GCGC^[CT]
578 GGCGCC
MboI 204 GATC ^GATC
412 GATC
MspA1I 19 CCGCTG C[AC]G^C[GT]G
78 CAGCGG
NdeII 204 GATC ^GATC
412 GATC
PaeR7I 61 CTCGAG C^TCGAG
614 CTCGAG
PstI 103 CTGCAG CTGCA^G
124 CTGCAG
Sau3AI 204 GATC ^GATC
412 GATC
SfaNI 561 GATGC GCATC(5/9)
588 GCATC
SfcI 103 CTGCAG C^T[GA][CT]AG
124 CTGCAG
SmaI 44 CCCGGG CCC^GGG
359 CCCGGG
XhoI 61 CTCGAG C^TCGAG
614 CTCGAG
XmaCI 44 CCCGGG C^CCGGG
359 CCCGGG
XmaI 44 CCCGGG C^CCGGG
359 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AvaI BanII BbvI
BcnI BglI BmyI BpmI BsaJI
BsoBI Bsp1286I BssKI BstF5I BstNI
Cac8I CfoI Cfr13I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MnlI MspI MvaI MwoI NciI
NlaIII NlaIV Sau96I ScrFI SmlI
StyI TaqI TseI TthHB8I