################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI Aor51HI ApoI AscI AseI AsnI Asp700I AvaII AvrII BalI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DrdI EarI Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HincII HindII HindIII HpaI Ksp632I KspI MaeI MamI MboII MfeI MluI MluNI MroI MscI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 492 GGTACC G^GTACC AhdI 222 GACCCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 376 GTGCAC G^TGCAC AlwNI 612 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 31 CCTGAGG CC^T[ACGT]AGG ApaLI 376 GTGCAC G^TGCAC Asp718I 492 GGTACC G^GTACC AspEI 222 GACCCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 130 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 602 TGCGCA TGC^GCA BamHI 56 GGATCC G^GATCC BbeI 83 GGCGCC GGCGC^C BseRI 256 GAGGAG GAGGAG(10/8) BsiEI 452 CGGCCG CG[GA][CT]^CG BsmAI 306 GAGAC GTCTC(1/5) BsrFI 450 GCCGGC [GA]^CCGG[CT] BsrI 101 CCAGT ACTGG(1/-1) BstXI 539 CCATCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 31 CCTGAGG CC^T[ACGT]AGG Cfr10I 450 GCCGGC [GA]^CCGG[CT] DdeI 32 CTGAG C^T[ACGT]AG DraIII 520 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 452 CGGCCG C^GGCCG Eam1105I 222 GACCCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 452 CGGCCG C^GGCCG Eco52I 452 CGGCCG C^GGCCG Eco81I 31 CCTGAGG CC^T[ACGT]AGG FauI 585 GCGGG CCCGC(4/6) FspI 602 TGCGCA TGC^GCA KasI 83 GGCGCC G^GCGCC KpnI 492 GGTACC GGTAC^C MaeII 42 ACGT A^CGT MaeIII 321 GTCAC ^GT[ACGT]AC NaeI 450 GCCGGC GCC^GGC NarI 83 GGCGCC GG^CGCC NgoMIV 450 GCCGGC G^CCGGC NspI 363 GCATGT [GA]CATG^[CT] PflFI 130 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 519 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 568 GAGCTC GAGCT^C SfoI 83 GGCGCC GGC^GCC Tsp45I 321 GTCAC ^GT[GC]AC Tth111I 130 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 519 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 83 GGCGCC G[GA]^CG[CT]C 181 GGCGTC AfaI 195 GTAC GT^AC 493 GTAC ApaI 405 GGGCCC GGGCC^C 528 GGGCCC AvaI 61 CCCGAG C^[CT]CG[GA]G 69 CCCGAG BanI 83 GGCGCC G^G[CT][GA]CC 492 GGTACC BcnI 123 CCCGG CC^[GC]GG 462 CCGGG BsaHI 83 GGCGCC G[GA]^CG[CT]C 181 GGCGTC BsgI 331 CTGCAC GTGCAG(16/14) 430 CTGCAC BsoBI 61 CCCGAG C^[CT]CG[GA]G 69 CCCGAG BspMI 359 ACCTGC ACCTGC(4/8) 428 ACCTGC BstF5I 411 CATCC GGATG(2/0) 201 GGATG BstYI 56 GGATCC [GA]^GATC[CT] 206 AGATCC DsaI 524 CCGTGG C^C[GA][CT]GG 545 CCGTGG EaeI 371 TGGCCG [CT]^GGCC[GA] 452 CGGCCG FokI 411 CATCC GGATG(9/13) 201 GGATG Hin1I 83 GGCGCC G[GA]^CG[CT]C 181 GGCGTC MflI 56 GGATCC [GA]^GATC[CT] 206 AGATCC MspA1I 79 CAGCGG C[AC]G^C[GT]G 612 CAGCGG NciI 123 CCCGG CC^[GC]GG 462 CCGGG RsaI 195 GTAC GT^AC 493 GTAC TaqI 152 TCGA T^CGA 254 TCGA TthHB8I 152 TCGA T^CGA 254 TCGA XhoII 56 GGATCC [GA]^GATC[CT] 206 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AccII AciI AluI AlwI AspHI BanII BbvI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DpnII DraII EcoO109I EcoRII Fnu4HI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MboI MnlI MslI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI TseI TspRI