Restriction map for
pF1KB6932
##################################
# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AciI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI Bsp1286I Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstUI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HhaI HinP1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MspA1I MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Van91I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccIII 71 TCCGGA T^CCGGA
AclI 668 AACGTT AA^CGTT
AcsI 451 AAATTC [GA]^AATT[CT]
AcyI 3 GGCGTC G[GA]^CG[CT]C
AfaI 270 GTAC GT^AC
AlwNI 653 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 451 AAATTC [GA]^AATT[CT]
Asp700I 163 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 541 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 283 CTCGAG C^[CT]CG[GA]G
BalI 47 TGGCCA TGG^CCA
BanI 384 GGCACC G^G[CT][GA]CC
BbsI 610 GAAGAC GAAGAC(2/6)
BclI 626 TGATCA T^GATCA
BcnI 433 CCGGG CC^[GC]GG
BglII 619 AGATCT A^GATCT
BpuAI 610 GAAGAC GAAGAC(2/6)
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsaWI 71 TCCGGA [AT]^CCGG[AT]
BseAI 71 TCCGGA T^CCGGA
BseRI 603 GAGGAG GAGGAG(10/8)
BsoBI 283 CTCGAG C^[CT]CG[GA]G
BspEI 71 TCCGGA T^CCGGA
BstBI 170 TTCGAA TT^CGAA
BstEII 148 GGTCACC G^GT[ACGT]ACC
BstPI 148 GGTCACC G^GT[ACGT]ACC
BstXI 226 CCAGAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 619 AGATCT [GA]^GATC[CT]
DraIII 151 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 388 CCATGG C^C[GA][CT]GG
EaeI 47 TGGCCA [CT]^GGCC[GA]
EcoO65I 148 GGTCACC G^GT[ACGT]ACC
FbaI 626 TGATCA T^GATCA
HgaI 4 GCGTC GACGC(5/10)
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HinfI 82 GACTC G^A[ACGT]TC
MaeIII 149 GTCAC ^GT[ACGT]AC
MflI 619 AGATCT [GA]^GATC[CT]
MluNI 47 TGGCCA TGG^CCA
MroI 71 TCCGGA T^CCGGA
MscI 47 TGGCCA TGG^CCA
NciI 433 CCGGG CC^[GC]GG
NcoI 388 CCATGG C^CATGG
NspI 203 ACATGC [GA]CATG^[CT]
NspV 170 TTCGAA TT^CGAA
PaeR7I 283 CTCGAG C^TCGAG
PflFI 541 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 82 GACTC GAGTC(4/5)
Psp1406I 668 AACGTT AA^CGTT
RsaI 270 GTAC GT^AC
SfcI 251 CTGTAG C^T[GA][CT]AG
SfuI 170 TTCGAA TT^CGAA
SmlI 283 CTCGAG C^T[CT][GA]AG
Tsp45I 149 GTCAC ^GT[GC]AC
Tth111I 541 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 472 CCAAAGGTTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 283 CTCGAG C^TCGAG
XhoII 619 AGATCT [GA]^GATC[CT]
XmnI 163 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BfaI 79 CTAG C^TAG
181 CTAG
BsgI 100 CTGCAC GTGCAG(16/14)
557 GTGCAG
BsrI 482 ACTGG ACTGG(1/-1)
495 CCAGT
EcoT14I 388 CCATGG C^C[AT][AT]GG
413 CCTTGG
HapII 72 CCGG C^CGG
433 CCGG
HpaII 72 CCGG C^CGG
433 CCGG
HphI 140 TCACC GGTGA(8/7)
150 TCACC
MaeI 79 CTAG C^TAG
181 CTAG
MaeII 110 ACGT A^CGT
669 ACGT
MboII 460 GAAGA GAAGA(8/7)
610 GAAGA
MseI 314 TTAA T^TAA
637 TTAA
MspI 72 CCGG C^CGG
433 CCGG
MwoI 14 GCAAGATTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
385 GCACCATGGGC
SfaNI 75 GATGC GCATC(5/9)
468 GATGC
StyI 388 CCATGG C^C[AT][AT]GG
413 CCTTGG
Tru9I 314 TTAA T^TAA
637 TTAA
Tsp509I 52 AATT ^AATT
452 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AluI AvaII BbvI BsaJI BsiYI
BslI BssKI BstF5I BstNI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII ItaI MboI
MnlI MslI MvaI NdeII NlaIII
NlaIV PpuMI Sau3AI Sau96I ScrFI
TaqI TseI TspRI TthHB8I