Restriction map for
pF1KB6935
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
Acc65I AccI AclI AfaI AflII
AflIII AhdI AlwI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsiEI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DpnII
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HgaI HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MaeIII MamI MboI MfeI
MflI MluI MluNI MscI MseI
MslI MunI NarI NciI NcoI
NdeI NdeII NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SalI Sau3AI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tru9I Tsp45I Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 111 GACGTC GACGT^C
AcsI 319 AAATTC [GA]^AATT[CT]
AcyI 111 GACGTC G[GA]^CG[CT]C
AgeI 521 ACCGGT A^CCGGT
Alw44I 183 GTGCAC G^TGCAC
AlwNI 76 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 183 GTGCAC G^TGCAC
ApoI 319 AAATTC [GA]^AATT[CT]
Asp700I 496 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BpmI 55 CTGGAG CTGGAG(16/14)
BsaHI 111 GACGTC G[GA]^CG[CT]C
BseRI 392 GAGGAG GAGGAG(10/8)
BsgI 82 CTGCAC GTGCAG(16/14)
BsmI 198 GAATGC GAATGC(1/-1)
BsrBI 581 GAGCGG CCGCTC(-3/-3)
BsrDI 397 GCAATG GCAATG(2/0)
CfoI 379 GCGC GCG^C
DdeI 552 CTCAG C^T[ACGT]AG
DraIII 436 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 99 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HhaI 379 GCGC GCG^C
Hin1I 111 GACGTC G[GA]^CG[CT]C
HinP1I 379 GCGC G^CGC
HinfI 274 GAGTC G^A[ACGT]TC
MaeII 112 ACGT A^CGT
NaeI 243 GCCGGC GCC^GGC
NgoMIV 243 GCCGGC G^CCGGC
NlaIII 511 CATG CATG^
PinAI 521 ACCGGT A^CCGGT
PleI 274 GAGTC GAGTC(4/5)
PpuMI 134 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 119 CAGCTG CAG^CTG
SacI 549 GAGCTC GAGCT^C
SapI 247 GCTCTTC GCTCTTC(1/4)
SmlI 574 CTCAAG C^T[CT][GA]AG
TaqI 227 TCGA T^CGA
TthHB8I 227 TCGA T^CGA
XmnI 496 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 378 CGCG CG^CG
380 CGCG
AccIII 165 TCCGGA T^CCGGA
475 TCCGGA
AspHI 183 GTGCAC G[AT]GC[AT]^C
549 GAGCTC
AvaI 217 CCCGAG C^[CT]CG[GA]G
594 CTCGGG
AvaII 135 GGTCC G^G[AT]CC
163 GGTCC
BseAI 165 TCCGGA T^CCGGA
475 TCCGGA
BsiHKAI 183 GTGCAC G[AT]GC[AT]^C
549 GAGCTC
BsoBI 217 CCCGAG C^[CT]CG[GA]G
594 CTCGGG
BspEI 165 TCCGGA T^CCGGA
475 TCCGGA
BsrFI 243 GCCGGC [GA]^CCGG[CT]
521 ACCGGT
BsrI 33 ACTGG ACTGG(1/-1)
483 ACTGG
BstAPI 75 GCAGGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
118 GCAGCTGATGC
BstUI 378 CGCG CG^CG
380 CGCG
Cfr10I 243 GCCGGC [GA]^CCGG[CT]
521 ACCGGT
DraII 134 AGGTCCC [GA]G^G[ACGT]CC[CT]
565 GGGGCCT
DsaI 128 CCGTGG C^C[GA][CT]GG
461 CCGTGG
EaeI 485 TGGCCG [CT]^GGCC[GA]
612 TGGCCG
EarI 248 CTCTTC CTCTTC(1/4)
229 GAAGAG
EcoO109I 134 AGGTCCC [GA]G^G[ACGT]CC[CT]
565 GGGGCCT
HphI 252 TCACC GGTGA(8/7)
281 TCACC
Ksp632I 248 CTCTTC CTCTTC(1/4)
229 GAAGAG
MboII 229 GAAGA GAAGA(8/7)
249 TCTTC
MroI 165 TCCGGA T^CCGGA
475 TCCGGA
MvnI 378 CGCG CG^CG
380 CGCG
PflMI 435 CCACAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
519 CCACCGGTTGG
PstI 116 CTGCAG CTGCA^G
447 CTGCAG
SfaNI 124 GATGC GCATC(5/9)
177 GATGC
SfcI 116 CTGCAG C^T[GA][CT]AG
447 CTGCAG
Tsp509I 320 AATT ^AATT
347 AATT
Van91I 435 CCACAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
519 CCACCGGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BsaJI BsaWI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I EcoRII FauI Fnu4HI FokI
HaeIII HapII HpaII ItaI MnlI
MspA1I MspI MvaI MwoI NlaIV
Sau96I ScrFI TseI TspRI