################################## # NO CUT ENZYMES ( 186 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiHKAI BsiWI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AlwI 114 GATCC GGATC(4/5) AlwNI 297 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 233 AGCGCT AGC^GCT AvaII 675 GGACC G^G[AT]CC BciVI 16 GGATAC GTATCC(6/5) BmyI 273 GGGCAC G[AGT]GC[ACT]^C BpmI 315 CTGGAG CTGGAG(16/14) BsaJI 668 CCAAGG C^C[ACGT][ACGT]GG BsaWI 425 ACCGGA [AT]^CCGG[AT] BseRI 78 CTCCTC GAGGAG(10/8) BsgI 387 CTGCAC GTGCAG(16/14) BsmAI 58 GTCTC GTCTC(1/5) BsmBI 57 CGTCTC CGTCTC(1/5) BsmI 399 GAATGC GAATGC(1/-1) Bsp1286I 273 GGGCAC G[AGT]GC[ACT]^C BsrI 314 ACTGG ACTGG(1/-1) Cac8I 658 GCTGGC GC[ACGT]^[ACGT]GC DdeI 534 CTCAG C^T[ACGT]AG DpnII 114 GATC ^GATC EarI 370 GAAGAG CTCTTC(1/4) Eco47III 233 AGCGCT AGC^GCT EcoT14I 668 CCAAGG C^C[AT][AT]GG HaeII 233 AGCGCT [GA]GCGC^[CT] HaeIII 67 GGCC GG^CC HapII 426 CCGG C^CGG HpaII 426 CCGG C^CGG HphI 23 TCACC GGTGA(8/7) Ksp632I 370 GAAGAG CTCTTC(1/4) MaeIII 305 GTGAC ^GT[ACGT]AC MboI 114 GATC ^GATC MslI 390 CACTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 426 CCGG C^CGG NdeII 114 GATC ^GATC PleI 456 GACTC GAGTC(4/5) Sau3AI 114 GATC ^GATC ScaI 383 AGTACT AGT^ACT SfaNI 73 GCATC GCATC(5/9) SspI 31 AATATT AAT^ATT StyI 668 CCAAGG C^C[AT][AT]GG TaqI 496 TCGA T^CGA TfiI 105 GATTC G^A[AT]TC Tsp45I 305 GTGAC ^GT[GC]AC TspRI 390 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 496 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AfaI 384 GTAC GT^AC 441 GTAC AluI 51 AGCT AG^CT 334 AGCT BssKI 83 CCTGG ^CC[ACGT]GG 452 CCAGG BstNI 83 CCTGG CC^[AT]GG 452 CCAGG CfoI 174 GCGC GCG^C 234 GCGC Cfr13I 67 GGCCC G^G[ACGT]CC 675 GGACC EcoRII 83 CCTGG ^CC[AT]GG 452 CCAGG HhaI 174 GCGC GCG^C 234 GCGC HinP1I 174 GCGC G^CGC 234 GCGC HinfI 105 GATTC G^A[ACGT]TC 456 GACTC MvaI 83 CCTGG CC^[AT]GG 452 CCAGG RsaI 384 GTAC GT^AC 441 GTAC Sau96I 67 GGCCC G^G[ACGT]CC 675 GGACC ScrFI 83 CCTGG CC^[ACGT]GG 452 CCAGG ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### BbvI BsiYI BslI BstF5I Fnu4HI FokI ItaI MboII MnlI MseI NlaIII Tru9I TseI Tsp509I