Restriction map for pF1KB6938
##################################
# NO CUT ENZYMES (  186 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BseAI       BsiEI       BsiHKAI     
BsiWI       BsmFI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MaeII       MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AlwI          114  GATCC                GGATC(4/5)
AlwNI         297  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       233  AGCGCT               AGC^GCT
AvaII         675  GGACC                G^G[AT]CC
BciVI          16  GGATAC               GTATCC(6/5)
BmyI          273  GGGCAC               G[AGT]GC[ACT]^C
BpmI          315  CTGGAG               CTGGAG(16/14)
BsaJI         668  CCAAGG               C^C[ACGT][ACGT]GG
BsaWI         425  ACCGGA               [AT]^CCGG[AT]
BseRI          78  CTCCTC               GAGGAG(10/8)
BsgI          387  CTGCAC               GTGCAG(16/14)
BsmAI          58  GTCTC                GTCTC(1/5)
BsmBI          57  CGTCTC               CGTCTC(1/5)
BsmI          399  GAATGC               GAATGC(1/-1)
Bsp1286I      273  GGGCAC               G[AGT]GC[ACT]^C
BsrI          314  ACTGG                ACTGG(1/-1)
Cac8I         658  GCTGGC               GC[ACGT]^[ACGT]GC
DdeI          534  CTCAG                C^T[ACGT]AG
DpnII         114  GATC                 ^GATC
EarI          370  GAAGAG               CTCTTC(1/4)
Eco47III      233  AGCGCT               AGC^GCT
EcoT14I       668  CCAAGG               C^C[AT][AT]GG
HaeII         233  AGCGCT               [GA]GCGC^[CT]
HaeIII         67  GGCC                 GG^CC
HapII         426  CCGG                 C^CGG
HpaII         426  CCGG                 C^CGG
HphI           23  TCACC                GGTGA(8/7)
Ksp632I       370  GAAGAG               CTCTTC(1/4)
MaeIII        305  GTGAC                ^GT[ACGT]AC
MboI          114  GATC                 ^GATC
MslI          390  CACTGACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          426  CCGG                 C^CGG
NdeII         114  GATC                 ^GATC
PleI          456  GACTC                GAGTC(4/5)
Sau3AI        114  GATC                 ^GATC
ScaI          383  AGTACT               AGT^ACT
SfaNI          73  GCATC                GCATC(5/9)
SspI           31  AATATT               AAT^ATT
StyI          668  CCAAGG               C^C[AT][AT]GG
TaqI          496  TCGA                 T^CGA
TfiI          105  GATTC                G^A[AT]TC
Tsp45I        305  GTGAC                ^GT[GC]AC
TspRI         390  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       496  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AfaI          384  GTAC                 GT^AC
              441  GTAC                
AluI           51  AGCT                 AG^CT
              334  AGCT                
BssKI          83  CCTGG                ^CC[ACGT]GG
              452  CCAGG               
BstNI          83  CCTGG                CC^[AT]GG
              452  CCAGG               
CfoI          174  GCGC                 GCG^C
              234  GCGC                
Cfr13I         67  GGCCC                G^G[ACGT]CC
              675  GGACC               
EcoRII         83  CCTGG                ^CC[AT]GG
              452  CCAGG               
HhaI          174  GCGC                 GCG^C
              234  GCGC                
HinP1I        174  GCGC                 G^CGC
              234  GCGC                
HinfI         105  GATTC                G^A[ACGT]TC
              456  GACTC               
MvaI           83  CCTGG                CC^[AT]GG
              452  CCAGG               
RsaI          384  GTAC                 GT^AC
              441  GTAC                
Sau96I         67  GGCCC                G^G[ACGT]CC
              675  GGACC               
ScrFI          83  CCTGG                CC^[ACGT]GG
              452  CCAGG               


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

BbvI        BsiYI       BslI        BstF5I      Fnu4HI      
FokI        ItaI        MboII       MnlI        MseI        
NlaIII      Tru9I       TseI        Tsp509I     
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