Restriction map for
pF1KB6941
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# NO CUT ENZYMES ( 186 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbvCI BbvI BcgI
BciVI BclI BcnI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1286I BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
Fnu4HI FseI FspI HaeIII HapII
HgaI Hin1I HincII HindII HindIII
HpaI HpaII ItaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
TaqI TseI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcsI 517 GAATTT [GA]^AATT[CT]
AfaI 442 GTAC GT^AC
AflII 340 CTTAAG C^TTAAG
AlwI 103 GGATC GGATC(4/5)
ApoI 517 GAATTT [GA]^AATT[CT]
BbrPI 576 CACGTG CAC^GTG
BbsI 559 GAAGAC GAAGAC(2/6)
BfrI 340 CTTAAG C^TTAAG
BpuAI 559 GAAGAC GAAGAC(2/6)
BsaAI 576 CACGTG [CT]AC^GT[GA]
BseRI 236 GAGGAG GAGGAG(10/8)
BsmAI 468 GAGAC GTCTC(1/5)
Bsp1407I 441 TGTACA T^GTACA
BspLU11I 438 ACATGT A^CATGT
BsrGI 441 TGTACA T^GTACA
BstYI 103 GGATCT [GA]^GATC[CT]
CfoI 325 GCGC GCG^C
EarI 116 GAAGAG CTCTTC(1/4)
EcoNI 399 CCTGAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 395 CCCGC CCCGC(4/6)
HaeII 324 GGCGCT [GA]GCGC^[CT]
HhaI 325 GCGC GCG^C
HinP1I 325 GCGC G^CGC
Ksp632I 116 GAAGAG CTCTTC(1/4)
MaeII 577 ACGT A^CGT
MaeIII 241 GTCAC ^GT[ACGT]AC
MflI 103 GGATCT [GA]^GATC[CT]
MslI 157 CATCGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 424 GCAGAAGTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 438 ACATGT [GA]CATG^[CT]
PmaCI 576 CACGTG CAC^GTG
PmlI 576 CACGTG CAC^GTG
RsaI 442 GTAC GT^AC
SmlI 340 CTTAAG C^T[CT][GA]AG
SspBI 441 TGTACA T^GTACA
Tsp45I 241 GTCAC ^GT[GC]AC
TspRI 503 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 103 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AciI 134 CCGC CCGC(-3/-1)
396 CCGC
AflIII 438 ACATGT A^C[GA][CT]GT
577 ACGTGT
AluI 128 AGCT AG^CT
458 AGCT
BfaI 215 CTAG C^TAG
553 CTAG
BsiYI 188 CCAGCCCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
399 CCTGAAAAAGG
BslI 188 CCAGCCCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
399 CCTGAAAAAGG
BsmFI 61 GTCCC GGGAC(10/14)
205 GTCCC
BssKI 193 CCTGG ^CC[ACGT]GG
246 CCAGG
BstF5I 281 CATCC GGATG(2/0)
609 GGATG
BstNI 193 CCTGG CC^[AT]GG
246 CCAGG
Cac8I 136 GCAGGC GC[ACGT]^[ACGT]GC
394 GCCCGC
DdeI 287 CTCAG C^T[ACGT]AG
564 CTCAG
DpnII 104 GATC ^GATC
259 GATC
EcoRII 193 CCTGG ^CC[AT]GG
246 CCAGG
FokI 281 CATCC GGATG(9/13)
609 GGATG
HphI 478 GGTGA GGTGA(8/7)
242 TCACC
MaeI 215 CTAG C^TAG
553 CTAG
MboI 104 GATC ^GATC
259 GATC
MvaI 193 CCTGG CC^[AT]GG
246 CCAGG
NdeII 104 GATC ^GATC
259 GATC
NlaIII 439 CATG CATG^
620 CATG
Sau3AI 104 GATC ^GATC
259 GATC
ScrFI 193 CCTGG CC^[ACGT]GG
246 CCAGG
SfaNI 610 GATGC GCATC(5/9)
97 GCATC
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
BsaJI BsrI HinfI MboII MnlI
MseI PleI TfiI Tru9I Tsp509I