##################################
# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BseAI BsgI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BsrI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DsaI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI HphI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MslI MspA1I
MunI NarI NcoI NdeI NheI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tru9I
Tsp509I Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 162 CGCG CG^CG
AcyI 672 GACGCC G[GA]^CG[CT]C
AlwI 464 GGATC GGATC(4/5)
AlwNI 494 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 171 GGGCCC GGGCC^C
AspHI 652 GTGCTC G[AT]GC[AT]^C
BanII 171 GGGCCC G[GA]GC[CT]^C
BpmI 406 CTGGAG CTGGAG(16/14)
BsaAI 514 TACGTG [CT]AC^GT[GA]
BsaHI 672 GACGCC G[GA]^CG[CT]C
BsaI 556 GAGACC GGTCTC(1/5)
BsaWI 461 ACCGGA [AT]^CCGG[AT]
BsiEI 71 CGGCCG CG[GA][CT]^CG
BsiHKAI 652 GTGCTC G[AT]GC[AT]^C
BsmAI 556 GAGAC GTCTC(1/5)
BsmI 478 GCATTC GAATGC(1/-1)
BspMI 138 ACCTGC ACCTGC(4/8)
BsrBI 36 GAGCGG CCGCTC(-3/-3)
BsrDI 267 CATTGC GCAATG(2/0)
BsrFI 142 GCCGGC [GA]^CCGG[CT]
BssSI 177 CACGAG CACGAG(-5/-1)
BstUI 162 CGCG CG^CG
Cfr10I 142 GCCGGC [GA]^CCGG[CT]
DraIII 567 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 672 GACGCCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 71 CGGCCG [CT]^GGCC[GA]
EagI 71 CGGCCG C^GGCCG
EclXI 71 CGGCCG C^GGCCG
Eco52I 71 CGGCCG C^GGCCG
EcoRV 502 GATATC GAT^ATC
EcoT14I 548 CCAAGG C^C[AT][AT]GG
HaeII 422 AGCGCC [GA]GCGC^[CT]
HgaI 672 GACGC GACGC(5/10)
Hin1I 672 GACGCC G[GA]^CG[CT]C
HinfI 544 GAATC G^A[ACGT]TC
MaeII 515 ACGT A^CGT
MvnI 162 CGCG CG^CG
NaeI 142 GCCGGC GCC^GGC
NgoMIV 142 GCCGGC G^CCGGC
NotI 70 GCGGCCGC GC^GGCCGC
PshAI 207 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
StyI 548 CCAAGG C^C[AT][AT]GG
TfiI 544 GAATC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AluI 347 AGCT AG^CT
590 AGCT
BanI 40 GGTGCC G^G[CT][GA]CC
135 GGCACC
BglI 43 GCCCCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
76 GCCTTCCTGGC
BstAPI 262 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
617 GCAAGCAGTGC
DpnII 453 GATC ^GATC
465 GATC
MaeIII 367 GTCAC ^GT[ACGT]AC
596 GTGAC
MboI 453 GATC ^GATC
465 GATC
MboII 444 GAAGA GAAGA(8/7)
20 TCTTC
NdeII 453 GATC ^GATC
465 GATC
PpuMI 62 AGGACCC [GA]G^G[AT]CC[CT]
401 AGGACCT
Sau3AI 453 GATC ^GATC
465 GATC
Tsp45I 367 GTCAC ^GT[GC]AC
596 GTGAC
TspRI 622 CAGTG CA[GC]TG[ACGT][ACGT]^
677 CACTG
XcmI 395 CCAAGAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
562 CCAGGCACAGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AvaII BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
MnlI MspI MvaI MwoI NciI
NlaIII NlaIV Sau96I ScrFI SfaNI
TseI