################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BseAI BsgI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BsrI BssHII Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DsaI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI NarI NcoI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccII 162 CGCG CG^CG AcyI 672 GACGCC G[GA]^CG[CT]C AlwI 464 GGATC GGATC(4/5) AlwNI 494 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 171 GGGCCC GGGCC^C AspHI 652 GTGCTC G[AT]GC[AT]^C BanII 171 GGGCCC G[GA]GC[CT]^C BpmI 406 CTGGAG CTGGAG(16/14) BsaAI 514 TACGTG [CT]AC^GT[GA] BsaHI 672 GACGCC G[GA]^CG[CT]C BsaI 556 GAGACC GGTCTC(1/5) BsaWI 461 ACCGGA [AT]^CCGG[AT] BsiEI 71 CGGCCG CG[GA][CT]^CG BsiHKAI 652 GTGCTC G[AT]GC[AT]^C BsmAI 556 GAGAC GTCTC(1/5) BsmI 478 GCATTC GAATGC(1/-1) BspMI 138 ACCTGC ACCTGC(4/8) BsrBI 36 GAGCGG CCGCTC(-3/-3) BsrDI 267 CATTGC GCAATG(2/0) BsrFI 142 GCCGGC [GA]^CCGG[CT] BssSI 177 CACGAG CACGAG(-5/-1) BstUI 162 CGCG CG^CG Cfr10I 142 GCCGGC [GA]^CCGG[CT] DraIII 567 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 672 GACGCCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 71 CGGCCG [CT]^GGCC[GA] EagI 71 CGGCCG C^GGCCG EclXI 71 CGGCCG C^GGCCG Eco52I 71 CGGCCG C^GGCCG EcoRV 502 GATATC GAT^ATC EcoT14I 548 CCAAGG C^C[AT][AT]GG HaeII 422 AGCGCC [GA]GCGC^[CT] HgaI 672 GACGC GACGC(5/10) Hin1I 672 GACGCC G[GA]^CG[CT]C HinfI 544 GAATC G^A[ACGT]TC MaeII 515 ACGT A^CGT MvnI 162 CGCG CG^CG NaeI 142 GCCGGC GCC^GGC NgoMIV 142 GCCGGC G^CCGGC NotI 70 GCGGCCGC GC^GGCCGC PshAI 207 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC StyI 548 CCAAGG C^C[AT][AT]GG TfiI 544 GAATC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AluI 347 AGCT AG^CT 590 AGCT BanI 40 GGTGCC G^G[CT][GA]CC 135 GGCACC BglI 43 GCCCCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 76 GCCTTCCTGGC BstAPI 262 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 617 GCAAGCAGTGC DpnII 453 GATC ^GATC 465 GATC MaeIII 367 GTCAC ^GT[ACGT]AC 596 GTGAC MboI 453 GATC ^GATC 465 GATC MboII 444 GAAGA GAAGA(8/7) 20 TCTTC NdeII 453 GATC ^GATC 465 GATC PpuMI 62 AGGACCC [GA]G^G[AT]CC[CT] 401 AGGACCT Sau3AI 453 GATC ^GATC 465 GATC Tsp45I 367 GTCAC ^GT[GC]AC 596 GTGAC TspRI 622 CAGTG CA[GC]TG[ACGT][ACGT]^ 677 CACTG XcmI 395 CCAAGAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 562 CCAGGCACAGAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AvaII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI SfaNI TseI