Restriction map for
pF1KB6943
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiHKAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DpnII DraI DraIII DrdI DsaI
Eam1105I EarI Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
HphI KpnI Ksp632I KspI MamI
MboI MfeI MflI MluI MluNI
MroI MscI MslI MspA1I MunI
NcoI NdeI NdeII NheI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI Sau3AI SbfI ScaI SfiI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI StuI SwaI Tsp509I
Van91I XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 205 CGCG CG^CG
AcyI 165 GGCGCC G[GA]^CG[CT]C
AflIII 299 ACATGT A^C[GA][CT]GT
Aor51HI 24 AGCGCT AGC^GCT
AspI 532 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 165 GGCGCC G^G[CT][GA]CC
BanII 471 GGGCTC G[GA]GC[CT]^C
BbeI 165 GGCGCC GGCGC^C
BglI 157 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 471 GGGCTC G[AGT]GC[ACT]^C
BsaHI 165 GGCGCC G[GA]^CG[CT]C
BseRI 99 GAGGAG GAGGAG(10/8)
BsgI 295 CTGCAC GTGCAG(16/14)
BsiEI 17 CGGCCG CG[GA][CT]^CG
Bsp1286I 471 GGGCTC G[AGT]GC[ACT]^C
BspLU11I 299 ACATGT A^CATGT
BsrI 113 ACTGG ACTGG(1/-1)
BstUI 205 CGCG CG^CG
EaeI 17 CGGCCG [CT]^GGCC[GA]
EagI 17 CGGCCG C^GGCCG
EclXI 17 CGGCCG C^GGCCG
Eco47III 24 AGCGCT AGC^GCT
Eco52I 17 CGGCCG C^GGCCG
EcoT14I 351 CCTTGG C^C[AT][AT]GG
FauI 198 CCCGC CCCGC(4/6)
HgaI 129 GCGTC GACGC(5/10)
Hin1I 165 GGCGCC G[GA]^CG[CT]C
KasI 165 GGCGCC G^GCGCC
MaeII 45 ACGT A^CGT
MaeIII 463 GTCAC ^GT[ACGT]AC
MseI 484 TTAA T^TAA
MvnI 205 CGCG CG^CG
NarI 165 GGCGCC GG^CGCC
NotI 16 GCGGCCGC GC^GGCCGC
PflFI 532 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 52 CTGCAG CTGCA^G
SexAI 533 ACCTGGT A^CC[AT]GGT
SfaNI 397 GCATC GCATC(5/9)
SfcI 52 CTGCAG C^T[GA][CT]AG
SfoI 165 GGCGCC GGC^GCC
SspI 224 AATATT AAT^ATT
StyI 351 CCTTGG C^C[AT][AT]GG
TaqI 289 TCGA T^CGA
TfiI 514 GATTC G^A[AT]TC
Tru9I 484 TTAA T^TAA
Tsp45I 463 GTCAC ^GT[GC]AC
TspRI 465 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 532 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 289 TCGA T^CGA
XbaI 75 TCTAGA T^CTAGA
XcmI 134 CCAAAGGCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AluI 293 AGCT AG^CT
367 AGCT
AvaI 158 CCCGGG C^[CT]CG[GA]G
213 CCCGGG
BbsI 492 GAAGAC GAAGAC(2/6)
538 GTCTTC
BfaI 76 CTAG C^TAG
175 CTAG
BpuAI 492 GAAGAC GAAGAC(2/6)
538 GTCTTC
BsmFI 451 GGGAC GGGAC(10/14)
131 GTCCC
BsoBI 158 CCCGGG C^[CT]CG[GA]G
213 CCCGGG
BsrFI 186 GCCGGC [GA]^CCGG[CT]
193 GCCGGC
BstF5I 347 CATCC GGATG(2/0)
398 CATCC
Cfr10I 186 GCCGGC [GA]^CCGG[CT]
193 GCCGGC
DdeI 410 CTGAG C^T[ACGT]AG
422 CTAAG
FokI 347 CATCC GGATG(9/13)
398 CATCC
HaeII 24 AGCGCT [GA]GCGC^[CT]
165 GGCGCC
MaeI 76 CTAG C^TAG
175 CTAG
MboII 492 GAAGA GAAGA(8/7)
539 TCTTC
NaeI 186 GCCGGC GCC^GGC
193 GCCGGC
NgoMIV 186 GCCGGC G^CCGGC
193 GCCGGC
PleI 495 GACTC GAGTC(4/5)
286 GAGTC
SmaI 158 CCCGGG CCC^GGG
213 CCCGGG
SphI 117 GCATGC GCATG^C
384 GCATGC
XmaCI 158 CCCGGG C^CCGGG
213 CCCGGG
XmaI 158 CCCGGG C^CCGGG
213 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AvaII BbvI BcnI BpmI
BsaJI BsiYI BslI BssKI BstNI
Cac8I CfoI Cfr13I DraII EcoO109I
EcoRII Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MnlI
MspI MvaI MwoI NciI NlaIII
NlaIV NspI PpuMI Sau96I ScrFI
TseI