Restriction map for
pF1KB6953
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HaeIII Hin1I HinfI HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MspA1I MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsaI RsrII SacII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tru9I TspRI Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 190 GTCTAC GT^[AC][GT]AC
AccII 87 CGCG CG^CG
AlwI 574 GATCC GGATC(4/5)
Aor51HI 152 AGCGCT AGC^GCT
Asp700I 500 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 229 CCCGAG C^[CT]CG[GA]G
AvrII 262 CCTAGG C^CTAGG
BbsI 394 GAAGAC GAAGAC(2/6)
BbvI 155 GCTGC GCAGC(8/12)
BlnI 262 CCTAGG C^CTAGG
BpuAI 394 GAAGAC GAAGAC(2/6)
BsaI 538 GAGACC GGTCTC(1/5)
BsiYI 446 CCAAGCTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 446 CCAAGCTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 538 GAGAC GTCTC(1/5)
BsmFI 168 GTCCC GGGAC(10/14)
BsoBI 229 CCCGAG C^[CT]CG[GA]G
BsrFI 143 GCCGGT [GA]^CCGG[CT]
BstUI 87 CGCG CG^CG
Cfr10I 143 GCCGGT [GA]^CCGG[CT]
DpnII 574 GATC ^GATC
Eco47III 152 AGCGCT AGC^GCT
FauI 104 GCGGG CCCGC(4/6)
HapII 144 CCGG C^CGG
HgaI 554 GCGTC GACGC(5/10)
HincII 220 GTCAAC GT[CT]^[GA]AC
HindII 220 GTCAAC GT[CT]^[GA]AC
HindIII 448 AAGCTT A^AGCTT
HpaII 144 CCGG C^CGG
HphI 94 GGTGA GGTGA(8/7)
MaeII 236 ACGT A^CGT
MaeIII 613 GTGAC ^GT[ACGT]AC
MboI 574 GATC ^GATC
MspI 144 CCGG C^CGG
MvnI 87 CGCG CG^CG
MwoI 155 GCTGCTCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 574 GATC ^GATC
NheI 334 GCTAGC G^CTAGC
NlaIV 137 GGCTCC GG[ACGT]^[ACGT]CC
Sau3AI 574 GATC ^GATC
SexAI 529 ACCAGGT A^CC[AT]GGT
SfaNI 323 GATGC GCATC(5/9)
TseI 155 GCTGC G^C[AT]GC
Tsp45I 613 GTGAC ^GT[GC]AC
XcmI 269 CCAAGAGAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 500 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
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AcsI 285 GAATTT [GA]^AATT[CT]
360 GAATTT
ApoI 285 GAATTT [GA]^AATT[CT]
360 GAATTT
AvaII 146 GGTCC G^G[AT]CC
266 GGTCC
BanII 50 GAGCTC G[GA]GC[CT]^C
79 GAGCTC
BfaI 263 CTAG C^TAG
335 CTAG
BlpI 76 GCTGAGC GC^T[ACGT]AGC
81 GCTCAGC
BpmI 53 CTCCAG CTGGAG(16/14)
597 CTCCAG
Bpu1102I 76 GCTGAGC GC^T[ACGT]AGC
81 GCTCAGC
BspHI 14 TCATGA T^CATGA
570 TCATGA
BsrI 413 ACTGG ACTGG(1/-1)
122 CCAGT
CelII 76 GCTGAGC GC^T[ACGT]AGC
81 GCTCAGC
Cfr13I 146 GGTCC G^G[ACGT]CC
266 GGTCC
Fnu4HI 135 GCGGC GC^[ACGT]GC
155 GCTGC
HaeII 73 GGCGCT [GA]GCGC^[CT]
152 AGCGCT
ItaI 135 GCGGC GC^[ACGT]GC
155 GCTGC
MaeI 263 CTAG C^TAG
335 CTAG
RcaI 14 TCATGA T^CATGA
570 TCATGA
SacI 50 GAGCTC GAGCT^C
79 GAGCTC
Sau96I 146 GGTCC G^G[ACGT]CC
266 GGTCC
Tsp509I 286 AATT ^AATT
361 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AciI AluI AspHI BmyI BsaJI
BsiHKAI Bsp1286I BssKI BstNI Cac8I
CfoI DdeI EcoRII EcoT14I HhaI
HinP1I MboII MnlI MvaI NlaIII
ScrFI StyI