Restriction map for
pF1KB6963
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# NO CUT ENZYMES ( 188 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BglII BlnI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstEII
BstF5I BstPI BstUI BstXI BstYI
Bsu36I CfoI Cfr10I ClaI CpoI
DpnII DraI DraII DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoT14I EcoT22I FauI
FbaI FokI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MaeIII
MamI MboI MflI MluI MluNI
MroI MscI MslI MspA1I MspI
MvnI NaeI NarI NciI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI StyI SwaI Tsp45I
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 60 GTATAC GT^[AC][GT]AC
AciI 10 CCGC CCGC(-3/-1)
AflII 501 CTTAAG C^TTAAG
BanI 154 GGCACC G^G[CT][GA]CC
BanII 53 GAGCCC G[GA]GC[CT]^C
BfaI 491 CTAG C^TAG
BfrI 501 CTTAAG C^TTAAG
BglI 341 GCCACGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 199 GCTGAGC GC^T[ACGT]AGC
BmyI 53 GAGCCC G[AGT]GC[ACT]^C
BpmI 80 CTCCAG CTGGAG(16/14)
Bpu1102I 199 GCTGAGC GC^T[ACGT]AGC
Bsp1286I 53 GAGCCC G[AGT]GC[ACT]^C
Bst1107I 60 GTATAC GTA^TAC
BstAPI 481 GCAAAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 593 TTCGAA TT^CGAA
BstZ17I 60 GTATAC GTA^TAC
CelII 199 GCTGAGC GC^T[ACGT]AGC
Cfr13I 348 GGGCC G^G[ACGT]CC
DsaI 342 CCACGG C^C[GA][CT]GG
EcoRV 460 GATATC GAT^ATC
HphI 106 GGTGA GGTGA(8/7)
MaeI 491 CTAG C^TAG
MaeII 64 ACGT A^CGT
MfeI 141 CAATTG C^AATTG
MunI 141 CAATTG C^AATTG
NspI 432 GCATGC [GA]CATG^[CT]
NspV 593 TTCGAA TT^CGAA
PflMI 140 CCAATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 634 GACTC GAGTC(4/5)
Sau96I 348 GGGCC G^G[ACGT]CC
SfuI 593 TTCGAA TT^CGAA
SphI 432 GCATGC GCATG^C
SspI 388 AATATT AAT^ATT
TfiI 211 GAATC G^A[AT]TC
TspRI 321 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 140 CCAATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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BbsI 631 GAAGAC GAAGAC(2/6)
46 GTCTTC
BpuAI 631 GAAGAC GAAGAC(2/6)
46 GTCTTC
BsrDI 227 CATTGC GCAATG(2/0)
605 GCAATG
BssKI 31 CCAGG ^CC[ACGT]GG
443 CCTGG
BstNI 31 CCAGG CC^[AT]GG
443 CCTGG
EcoRII 31 CCAGG ^CC[AT]GG
443 CCTGG
HaeIII 349 GGCC GG^CC
525 GGCC
HinfI 211 GAATC G^A[ACGT]TC
634 GACTC
MvaI 31 CCAGG CC^[AT]GG
443 CCTGG
NlaIV 154 GGCACC GG[ACGT]^[ACGT]CC
347 GGGGCC
ScrFI 31 CCAGG CC^[ACGT]GG
443 CCTGG
SfaNI 586 GATGC GCATC(5/9)
556 GCATC
SmlI 255 CTTGAG C^T[CT][GA]AG
501 CTTAAG
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
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AluI BbvI BsaJI BsiYI BslI
BsrI Cac8I DdeI Fnu4HI ItaI
MboII MnlI MseI MwoI NlaIII
TaqI Tru9I TseI Tsp509I TthHB8I