Restriction map for
pF1KB6964
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AclI AcsI AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BglI BglII BlnI BsaAI
BsaBI BsaHI BsaI BseRI BsgI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmBI BsoBI BspDI BspHI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
BstBI BstEII BstPI Bsu36I Cfr10I
ClaI CpoI DraI DraIII EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I MaeI MamI
MluI MluNI MscI MslI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccIII 662 TCCGGA T^CCGGA
AflII 711 CTTAAG C^TTAAG
AlwNI 520 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 69 GGGCCC GGGCC^C
BanII 69 GGGCCC G[GA]GC[CT]^C
BbsI 616 GTCTTC GAAGAC(2/6)
BfrI 711 CTTAAG C^TTAAG
BlpI 500 GCTCAGC GC^T[ACGT]AGC
BmyI 69 GGGCCC G[AGT]GC[ACT]^C
Bpu1102I 500 GCTCAGC GC^T[ACGT]AGC
BpuAI 616 GTCTTC GAAGAC(2/6)
BsaWI 662 TCCGGA [AT]^CCGG[AT]
BseAI 662 TCCGGA T^CCGGA
BsmFI 653 GGGAC GGGAC(10/14)
BsmI 312 GCATTC GAATGC(1/-1)
Bsp1286I 69 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 644 TGTACA T^GTACA
BspEI 662 TCCGGA T^CCGGA
BsrDI 352 GCAATG GCAATG(2/0)
BsrGI 644 TGTACA T^GTACA
Bst1107I 445 GTATAC GTA^TAC
BstXI 596 CCATGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 665 GGATCT [GA]^GATC[CT]
BstZ17I 445 GTATAC GTA^TAC
Cac8I 67 GCGGGC GC[ACGT]^[ACGT]GC
CelII 500 GCTCAGC GC^T[ACGT]AGC
CfoI 381 GCGC GCG^C
DraII 69 GGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 226 GACAAAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 65 CCGCGG C^C[GA][CT]GG
EcoO109I 69 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 584 CCTTGG C^C[AT][AT]GG
HaeII 380 GGCGCT [GA]GCGC^[CT]
HapII 663 CCGG C^CGG
HgaI 655 GACGC GACGC(5/10)
HhaI 381 GCGC GCG^C
HinP1I 381 GCGC G^CGC
HinfI 481 GACTC G^A[ACGT]TC
HpaII 663 CCGG C^CGG
HphI 606 GGTGA GGTGA(8/7)
KspI 65 CCGCGG CCGC^GG
MaeII 98 ACGT A^CGT
MflI 665 GGATCT [GA]^GATC[CT]
MroI 662 TCCGGA T^CCGGA
MspI 663 CCGG C^CGG
PleI 481 GACTC GAGTC(4/5)
PvuII 79 CAGCTG CAG^CTG
SacII 65 CCGCGG CCGC^GG
SexAI 536 ACCAGGT A^CC[AT]GGT
SfaNI 242 GATGC GCATC(5/9)
SmlI 711 CTTAAG C^T[CT][GA]AG
SspBI 644 TGTACA T^GTACA
StyI 584 CCTTGG C^C[AT][AT]GG
TaqI 165 TCGA T^CGA
Tsp45I 676 GTGAC ^GT[GC]AC
TspRI 648 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 165 TCGA T^CGA
XhoII 665 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 360 GTCTAC GT^[AC][GT]AC
445 GTATAC
AccII 66 CGCG CG^CG
194 CGCG
AfaI 113 GTAC GT^AC
645 GTAC
AluI 80 AGCT AG^CT
451 AGCT
AlwI 457 GATCC GGATC(4/5)
665 GGATC
BpmI 131 CTGGAG CTGGAG(16/14)
494 CTGGAG
BsaJI 65 CCGCGG C^C[ACGT][ACGT]GG
584 CCTTGG
BssKI 31 CCTGG ^CC[ACGT]GG
537 CCAGG
BstAPI 145 GCAATTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
343 GCAATTGCTGC
BstF5I 47 GGATG GGATG(2/0)
241 GGATG
BstNI 31 CCTGG CC^[AT]GG
537 CCAGG
BstUI 66 CGCG CG^CG
194 CGCG
Cfr13I 69 GGGCC G^G[ACGT]CC
70 GGCCC
DdeI 501 CTCAG C^T[ACGT]AG
632 CTGAG
DpnII 457 GATC ^GATC
666 GATC
EcoRII 31 CCTGG ^CC[AT]GG
537 CCAGG
FauI 67 GCGGG CCCGC(4/6)
195 GCGGG
FokI 47 GGATG GGATG(9/13)
241 GGATG
HaeIII 70 GGCC GG^CC
327 GGCC
MaeIII 212 GTTAC ^GT[ACGT]AC
676 GTGAC
MboI 457 GATC ^GATC
666 GATC
MvaI 31 CCTGG CC^[AT]GG
537 CCAGG
MvnI 66 CGCG CG^CG
194 CGCG
NdeII 457 GATC ^GATC
666 GATC
NspI 554 ACATGC [GA]CATG^[CT]
570 GCATGT
RsaI 113 GTAC GT^AC
645 GTAC
Sau3AI 457 GATC ^GATC
666 GATC
Sau96I 69 GGGCC G^G[ACGT]CC
70 GGCCC
ScrFI 31 CCTGG CC^[ACGT]GG
537 CCAGG
SfcI 11 CTACAG C^T[GA][CT]AG
517 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AciI BbvI BsmAI BsrI Fnu4HI
ItaI MboII MfeI MnlI MseI
MspA1I MunI MwoI NlaIII NlaIV
Tru9I TseI Tsp509I