Restriction map for
pF1KB6965
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BanII BbrPI BbsI BbvCI BbvI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmAI
BsmBI BspDI BspEI BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MaeI MamI MfeI MflI MluI
MroI MseI MspA1I MunI MvnI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI TfiI
Tru9I TseI Tsp509I Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 507 GTCTAC GT^[AC][GT]AC
AcyI 500 GGCGCC G[GA]^CG[CT]C
AflIII 470 ACATGT A^C[GA][CT]GT
AlwI 204 GATCC GGATC(4/5)
AlwNI 118 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 439 GTGCTC G[AT]GC[AT]^C
AvaI 191 CCCGGG C^[CT]CG[GA]G
BanI 500 GGCGCC G^G[CT][GA]CC
BbeI 500 GGCGCC GGCGC^C
BglI 187 GCCACCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 439 GTGCTC G[AGT]GC[ACT]^C
BpmI 359 CTGGAG CTGGAG(16/14)
BsaHI 500 GGCGCC G[GA]^CG[CT]C
BsiHKAI 439 GTGCTC G[AT]GC[AT]^C
BsoBI 191 CCCGGG C^[CT]CG[GA]G
Bsp1286I 439 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 2 TGTACA T^GTACA
BspLU11I 470 ACATGT A^CATGT
BspMI 155 ACCTGC ACCTGC(4/8)
BsrGI 2 TGTACA T^GTACA
BstAPI 143 GCAACAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 47 CCATCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 204 GATC ^GATC
DsaI 65 CCATGG C^C[GA][CT]GG
Hin1I 500 GGCGCC G[GA]^CG[CT]C
HinfI 362 GAGTC G^A[ACGT]TC
HphI 365 TCACC GGTGA(8/7)
KasI 500 GGCGCC G^GCGCC
MaeII 448 ACGT A^CGT
MaeIII 364 GTCAC ^GT[ACGT]AC
MboI 204 GATC ^GATC
MboII 452 TCTTC GAAGA(8/7)
MslI 234 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 497 GCCGGC GCC^GGC
NarI 500 GGCGCC GG^CGCC
NcoI 65 CCATGG C^CATGG
NdeII 204 GATC ^GATC
NgoMIV 497 GCCGGC G^CCGGC
NspI 470 ACATGT [GA]CATG^[CT]
PleI 362 GAGTC GAGTC(4/5)
PpuMI 103 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 157 CTGCAG CTGCA^G
Sau3AI 204 GATC ^GATC
SfcI 157 CTGCAG C^T[GA][CT]AG
SfoI 500 GGCGCC GGC^GCC
SmaI 191 CCCGGG CCC^GGG
SspBI 2 TGTACA T^GTACA
Tsp45I 364 GTCAC ^GT[GC]AC
XcmI 47 CCATCCTCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 191 CCCGGG C^CCGGG
XmaI 191 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AluI 7 AGCT AG^CT
373 AGCT
BalI 62 TGGCCA TGG^CCA
347 TGGCCA
BsmFI 43 GGGAC GGGAC(10/14)
395 GGGAC
BsmI 183 GAATGC GAATGC(1/-1)
493 GAATGC
BspHI 200 TCATGA T^CATGA
443 TCATGA
BsrFI 92 ACCGGC [GA]^CCGG[CT]
497 GCCGGC
Cac8I 301 GCTGGC GC[ACGT]^[ACGT]GC
497 GCCGGC
Cfr10I 92 ACCGGC [GA]^CCGG[CT]
497 GCCGGC
DraII 103 GGGTCCT [GA]G^G[ACGT]CC[CT]
119 AGGGCCT
EaeI 62 TGGCCA [CT]^GGCC[GA]
347 TGGCCA
EcoO109I 103 GGGTCCT [GA]G^G[ACGT]CC[CT]
119 AGGGCCT
EcoT14I 65 CCATGG C^C[AT][AT]GG
344 CCTTGG
FauI 278 CCCGC CCCGC(4/6)
515 CCCGC
Fnu4HI 383 GCCGC GC^[ACGT]GC
386 GCCGC
HaeII 401 GGCGCT [GA]GCGC^[CT]
500 GGCGCC
ItaI 383 GCCGC GC^[ACGT]GC
386 GCCGC
MluNI 62 TGGCCA TGG^CCA
347 TGGCCA
MnlI 51 CCTC CCTC(7/6)
320 CCTC
MscI 62 TGGCCA TGG^CCA
347 TGGCCA
RcaI 200 TCATGA T^CATGA
443 TCATGA
StyI 65 CCATGG C^C[AT][AT]GG
344 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AfaI AvaII BcnI BsaJI
BsiYI BslI BsrI BssKI BstF5I
BstNI CfoI Cfr13I EcoRII FokI
HaeIII HapII HhaI HinP1I HpaII
MspI MvaI MwoI NciI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI
TspRI