Restriction map for
pF1KB6969
##################################
# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HpaI KasI KpnI Ksp632I
KspI MaeI MamI MluI MroI
MslI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TfiI Tsp45I Tth111I TthHB8I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 393 CGCG CG^CG
AcsI 433 GAATTC [GA]^AATT[CT]
AlwNI 38 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 278 GGGCCC GGGCC^C
ApoI 433 GAATTC [GA]^AATT[CT]
BalI 413 TGGCCA TGG^CCA
BsmAI 484 GAGAC GTCTC(1/5)
BsmI 564 GAATGC GAATGC(1/-1)
BssSI 139 CACGAG CACGAG(-5/-1)
BstF5I 195 GGATG GGATG(2/0)
BstUI 393 CGCG CG^CG
CfoI 17 GCGC GCG^C
DdeI 536 CTAAG C^T[ACGT]AG
DraII 277 GGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 199 GACACAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 428 CCGTGG C^C[GA][CT]GG
EaeI 413 TGGCCA [CT]^GGCC[GA]
EcoO109I 277 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 433 GAATTC G^AATTC
FokI 195 GGATG GGATG(9/13)
HapII 627 CCGG C^CGG
HhaI 17 GCGC GCG^C
HinP1I 17 GCGC G^CGC
HindIII 541 AAGCTT A^AGCTT
HinfI 85 GAGTC G^A[ACGT]TC
HpaII 627 CCGG C^CGG
MaeII 509 ACGT A^CGT
MaeIII 398 GTAAC ^GT[ACGT]AC
MboII 521 TCTTC GAAGA(8/7)
MfeI 158 CAATTG C^AATTG
MluNI 413 TGGCCA TGG^CCA
MscI 413 TGGCCA TGG^CCA
MseI 65 TTAA T^TAA
MspI 627 CCGG C^CGG
MunI 158 CAATTG C^AATTG
MvnI 393 CGCG CG^CG
PleI 85 GAGTC GAGTC(4/5)
PstI 639 CTGCAG CTGCA^G
Tru9I 65 TTAA T^TAA
TspRI 634 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 561 CCAGAATGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 144 GTAC GT^AC
267 GTAC
AspHI 153 GAGCAC G[AT]GC[AT]^C
575 GTGCTC
AvaII 34 GGACC G^G[AT]CC
600 GGACC
BanII 278 GGGCCC G[GA]GC[CT]^C
556 GAGCCC
BglII 53 AGATCT A^GATCT
218 AGATCT
BpmI 578 CTCCAG CTGGAG(16/14)
604 CTCCAG
BsiHKAI 153 GAGCAC G[AT]GC[AT]^C
575 GTGCTC
BsmFI 129 GGGAC GGGAC(10/14)
224 GGGAC
BsrBI 164 GAGCGG CCGCTC(-3/-3)
241 GAGCGG
BsrDI 384 CATTGC GCAATG(2/0)
502 GCAATG
BstAPI 493 GCAAAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
568 GCAACTGGTGC
BstYI 53 AGATCT [GA]^GATC[CT]
218 AGATCT
DpnII 54 GATC ^GATC
219 GATC
FauI 4 GCGGG CCCGC(4/6)
394 GCGGG
HphI 345 GGTGA GGTGA(8/7)
308 TCACC
MboI 54 GATC ^GATC
219 GATC
MflI 53 AGATCT [GA]^GATC[CT]
218 AGATCT
MspA1I 38 CAGCTG C[AC]G^C[GT]G
230 CAGCTG
NdeII 54 GATC ^GATC
219 GATC
PflMI 37 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
335 CCAAGAACTGG
PvuII 38 CAGCTG CAG^CTG
230 CAGCTG
RsaI 144 GTAC GT^AC
267 GTAC
Sau3AI 54 GATC ^GATC
219 GATC
SfcI 355 CTACAG C^T[GA][CT]AG
639 CTGCAG
Van91I 37 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
335 CCAAGAACTGG
XhoII 53 AGATCT [GA]^GATC[CT]
218 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI Cac8I Cfr13I EcoRII Fnu4HI
HaeIII ItaI MnlI MvaI MwoI
NlaIII NlaIV Sau96I ScrFI TseI
Tsp509I