Restriction map for
pF1KB6970
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaJI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaI KasI KpnI
KspI MaeII MluI MroI MslI
MvnI NaeI NarI NcoI NgoMIV
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI TfiI Tth111I
Van91I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcsI 672 GAATTT [GA]^AATT[CT]
AflIII 680 ACATGT A^C[GA][CT]GT
ApoI 672 GAATTT [GA]^AATT[CT]
Asp700I 158 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 534 GAGCTC G[AT]GC[AT]^C
AvaI 500 CTCGAG C^[CT]CG[GA]G
BalI 666 TGGCCA TGG^CCA
BamHI 116 GGATCC G^GATCC
BbsI 588 GTCTTC GAAGAC(2/6)
BcnI 11 CCCGG CC^[GC]GG
BglI 79 GCCATTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 588 GTCTTC GAAGAC(2/6)
BsaBI 379 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 658 GTGCAG GTGCAG(16/14)
BsiHKAI 534 GAGCTC G[AT]GC[AT]^C
BsmI 630 GAATGC GAATGC(1/-1)
BsoBI 500 CTCGAG C^[CT]CG[GA]G
Bsp1407I 486 TGTACA T^GTACA
BspLU11I 680 ACATGT A^CATGT
BsrGI 486 TGTACA T^GTACA
BsrI 296 ACTGG ACTGG(1/-1)
BstF5I 600 CATCC GGATG(2/0)
Cac8I 648 GCTAGC GC[ACGT]^[ACGT]GC
EaeI 666 TGGCCA [CT]^GGCC[GA]
FokI 600 CATCC GGATG(9/13)
HaeIII 667 GGCC GG^CC
HapII 12 CCGG C^CGG
HindIII 94 AAGCTT A^AGCTT
HpaII 12 CCGG C^CGG
HphI 302 TCACC GGTGA(8/7)
MaeIII 334 GTGAC ^GT[ACGT]AC
MamI 379 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 506 CAATTG C^AATTG
MluNI 666 TGGCCA TGG^CCA
MscI 666 TGGCCA TGG^CCA
MseI 262 TTAA T^TAA
MspA1I 454 CAGCTG C[AC]G^C[GT]G
MspI 12 CCGG C^CGG
MunI 506 CAATTG C^AATTG
MwoI 79 GCCATTGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 11 CCCGG CC^[GC]GG
NdeI 463 CATATG CA^TATG
NheI 648 GCTAGC G^CTAGC
NspI 680 ACATGT [GA]CATG^[CT]
PaeR7I 500 CTCGAG C^TCGAG
PvuII 454 CAGCTG CAG^CTG
SacI 534 GAGCTC GAGCT^C
SfcI 104 CTACAG C^T[GA][CT]AG
SmlI 500 CTCGAG C^T[CT][GA]AG
SspBI 486 TGTACA T^GTACA
TaqI 501 TCGA T^CGA
Tru9I 262 TTAA T^TAA
Tsp45I 334 GTGAC ^GT[GC]AC
TthHB8I 501 TCGA T^CGA
XhoI 500 CTCGAG C^TCGAG
XmnI 158 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 21 GTAC GT^AC
487 GTAC
BanI 391 GGTGCC G^G[CT][GA]CC
526 GGTGCC
BanII 178 GAGCCC G[GA]GC[CT]^C
534 GAGCTC
BbvI 185 GCAGC GCAGC(8/12)
660 GCAGC
BfaI 142 CTAG C^TAG
649 CTAG
BseRI 431 GAGGAG GAGGAG(10/8)
619 GAGGAG
BsmFI 478 GGGAC GGGAC(10/14)
404 GTCCC
BstNI 388 CCAGG CC^[AT]GG
664 CCTGG
BstYI 116 GGATCC [GA]^GATC[CT]
384 AGATCC
DdeI 17 CTGAG C^T[ACGT]AG
254 CTAAG
DpnII 117 GATC ^GATC
385 GATC
DraII 447 AGGACCC [GA]G^G[ACGT]CC[CT]
478 GGGACCT
EarI 153 GAAGAG CTCTTC(1/4)
703 GAAGAG
EcoO109I 447 AGGACCC [GA]G^G[ACGT]CC[CT]
478 GGGACCT
EcoRII 388 CCAGG ^CC[AT]GG
664 CCTGG
Fnu4HI 185 GCAGC GC^[ACGT]GC
660 GCAGC
ItaI 185 GCAGC GC^[ACGT]GC
660 GCAGC
Ksp632I 153 GAAGAG CTCTTC(1/4)
703 GAAGAG
MaeI 142 CTAG C^TAG
649 CTAG
MboI 117 GATC ^GATC
385 GATC
MflI 116 GGATCC [GA]^GATC[CT]
384 AGATCC
MvaI 388 CCAGG CC^[AT]GG
664 CCTGG
NdeII 117 GATC ^GATC
385 GATC
PpuMI 447 AGGACCC [GA]G^G[AT]CC[CT]
478 GGGACCT
RsaI 21 GTAC GT^AC
487 GTAC
Sau3AI 117 GATC ^GATC
385 GATC
TseI 185 GCAGC G^C[AT]GC
660 GCAGC
XhoII 116 GGATCC [GA]^GATC[CT]
384 AGATCC
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AluI AlwI AvaII BmyI BsiYI
BslI BsmAI Bsp1286I BssKI Cfr13I
MboII MnlI NlaIII NlaIV Sau96I
ScrFI SfaNI Tsp509I TspRI