Restriction map for
pF1KB6979
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI AlwNI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BbeI
BbrPI BbvCI BcgI BciVI BfaI
BfrI BglI BglII BlnI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MunI MwoI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 508 CGCG CG^CG
Alw44I 375 GTGCAC G^TGCAC
AocI 151 CCTCAGG CC^T[ACGT]AGG
ApaLI 375 GTGCAC G^TGCAC
BamHI 452 GGATCC G^GATCC
BbsI 60 GAAGAC GAAGAC(2/6)
BclI 395 TGATCA T^GATCA
BlpI 174 GCTGAGC GC^T[ACGT]AGC
BpmI 576 CTGGAG CTGGAG(16/14)
Bpu1102I 174 GCTGAGC GC^T[ACGT]AGC
BpuAI 60 GAAGAC GAAGAC(2/6)
BsaAI 647 TACGTG [CT]AC^GT[GA]
BspHI 56 TCATGA T^CATGA
BspLU11I 293 ACATGT A^CATGT
BspMI 581 GCAGGT ACCTGC(4/8)
BsrBI 436 GAGCGG CCGCTC(-3/-3)
BstUI 508 CGCG CG^CG
BstYI 452 GGATCC [GA]^GATC[CT]
Bsu36I 151 CCTCAGG CC^T[ACGT]AGG
CelII 174 GCTGAGC GC^T[ACGT]AGC
DsaI 245 CCGTGG C^C[GA][CT]GG
Eco81I 151 CCTCAGG CC^T[ACGT]AGG
EcoT14I 380 CCAAGG C^C[AT][AT]GG
FbaI 395 TGATCA T^GATCA
HgaI 160 GCGTC GACGC(5/10)
MaeII 648 ACGT A^CGT
MflI 452 GGATCC [GA]^GATC[CT]
MspA1I 287 CAGCTG C[AC]G^C[GT]G
MvnI 508 CGCG CG^CG
NspI 293 ACATGT [GA]CATG^[CT]
PpuMI 561 GGGACCC [GA]G^G[AT]CC[CT]
PstI 226 CTGCAG CTGCA^G
PvuII 287 CAGCTG CAG^CTG
RcaI 56 TCATGA T^CATGA
SfcI 226 CTGCAG C^T[GA][CT]AG
StyI 380 CCAAGG C^C[AT][AT]GG
TaqI 99 TCGA T^CGA
Tsp45I 585 GTCAC ^GT[GC]AC
TspRI 589 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 99 TCGA T^CGA
XcmI 456 CCAAGACCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 452 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 293 ACATGT A^C[GA][CT]GT
648 ACGTGT
ApaI 34 GGGCCC GGGCC^C
68 GGGCCC
AspHI 361 GTGCTC G[AT]GC[AT]^C
375 GTGCAC
AvaI 127 CCCGAG C^[CT]CG[GA]G
263 CCCGAG
BanII 34 GGGCCC G[GA]GC[CT]^C
68 GGGCCC
BsiHKAI 361 GTGCTC G[AT]GC[AT]^C
375 GTGCAC
BsmAI 477 GAGAC GTCTC(1/5)
185 GTCTC
BsoBI 127 CCCGAG C^[CT]CG[GA]G
263 CCCGAG
BstXI 350 CCAGCAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
551 CCACTCCTCTGG
Cac8I 132 GCTGGC GC[ACGT]^[ACGT]GC
349 GCCAGC
CfoI 192 GCGC GCG^C
509 GCGC
FauI 547 CCCGC CCCGC(4/6)
29 GCGGG
HhaI 192 GCGC GCG^C
509 GCGC
HinP1I 192 GCGC G^CGC
509 GCGC
HinfI 256 GACTC G^A[ACGT]TC
479 GACTC
MaeIII 215 GTAAC ^GT[ACGT]AC
585 GTCAC
MboII 60 GAAGA GAAGA(8/7)
608 TCTTC
MslI 378 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
587 CACAGTGGTG
PleI 256 GACTC GAGTC(4/5)
479 GACTC
SfaNI 157 GATGC GCATC(5/9)
194 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AlwI AvaII BanI
BbvI BcnI BmyI BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MboI MnlI MspI MvaI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SmlI TseI