Restriction map for
pF1KB6980
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AfaI AflII AflIII AgeI
AlwNI Aor51HI ApoI AseI AsnI
Asp700I Asp718I AspI AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BcgI BclI BfrI BglII BlnI
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI Bst1107I BstEII
BstPI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoT22I FbaI FseI
HaeII HgaI Hin1I HincII HindII
HindIII HinfI HpaI KasI KpnI
Ksp632I KspI MaeII MaeIII MboII
MfeI MluI MluNI MroI MscI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XcmI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 154 GTCTAC GT^[AC][GT]AC
AhdI 277 GACCGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 670 GTGCAC G^TGCAC
AocI 575 CCTCAGG CC^T[ACGT]AGG
ApaI 206 GGGCCC GGGCC^C
ApaLI 670 GTGCAC G^TGCAC
AscI 50 GGCGCGCC GG^CGCGCC
AspEI 277 GACCGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 65 TGCGCA TGC^GCA
BbvCI 516 CCTCAGC CCTCAGC(-5/-2)
BciVI 449 GGATAC GTATCC(6/5)
BfaI 211 CTAG C^TAG
BglI 424 GCCACGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 479 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 479 GCTCAGC GC^T[ACGT]AGC
BsaBI 360 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 461 GAGAC GTCTC(1/5)
BsmI 417 GAATGC GAATGC(1/-1)
BspMI 375 GCAGGT ACCTGC(4/8)
BsrBI 631 GAGCGG CCGCTC(-3/-3)
BsrI 144 ACTGG ACTGG(1/-1)
BssHII 51 GCGCGC G^CGCGC
BstAPI 699 GCAGCATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 319 TTCGAA TT^CGAA
BstXI 137 CCAAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 575 CCTCAGG CC^T[ACGT]AGG
CelII 479 GCTCAGC GC^T[ACGT]AGC
EaeI 265 CGGCCA [CT]^GGCC[GA]
Eam1105I 277 GACCGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 575 CCTCAGG CC^T[ACGT]AGG
EcoRV 364 GATATC GAT^ATC
EcoT14I 137 CCAAGG C^C[AT][AT]GG
FspI 65 TGCGCA TGC^GCA
HphI 250 GGTGA GGTGA(8/7)
MaeI 211 CTAG C^TAG
MamI 360 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 703 CATGTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 702 GCATGT [GA]CATG^[CT]
NspV 319 TTCGAA TT^CGAA
PaeR7I 188 CTCGAG C^TCGAG
PpuMI 397 GGGACCT [GA]G^G[AT]CC[CT]
PstI 301 CTGCAG CTGCA^G
SacI 13 GAGCTC GAGCT^C
SfuI 319 TTCGAA TT^CGAA
SmaI 182 CCCGGG CCC^GGG
StuI 713 AGGCCT AGG^CCT
StyI 137 CCAAGG C^C[AT][AT]GG
XhoI 188 CTCGAG C^TCGAG
XmaCI 182 CCCGGG C^CCGGG
XmaI 182 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AlwI 72 GATCC GGATC(4/5)
171 GATCC
BpmI 10 CTGGAG CTGGAG(16/14)
628 CTGGAG
BssSI 166 CACGAG CACGAG(-5/-1)
616 CACGAG
BstYI 71 AGATCC [GA]^GATC[CT]
170 AGATCC
MflI 71 AGATCC [GA]^GATC[CT]
170 AGATCC
SfaNI 442 GATGC GCATC(5/9)
604 GATGC
SfcI 301 CTGCAG C^T[GA][CT]AG
382 CTACAG
SmlI 188 CTCGAG C^T[CT][GA]AG
595 CTTGAG
TspRI 143 CACTG CA[GC]TG[ACGT][ACGT]^
434 CAGTG
XhoII 71 AGATCC [GA]^GATC[CT]
170 AGATCC
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
AvaII BanII BbvI BcnI BmyI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MboI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PvuII Sau3AI Sau96I ScrFI TaqI
TseI TthHB8I