Restriction map for
pF1KB6994
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BanI BanII BbeI BbrPI
BcgI BciVI BcnI BfrI BglI
BlnI BmyI BsaAI BsaBI BsaHI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
BsrI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoT14I EcoT22I FauI
FseI FspI HaeII HgaI Hin1I
HincII HindII HinfI HpaI KasI
KpnI MaeII MamI MfeI MluI
MroI MslI MunI NarI NciI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TaqI TfiI Tth111I
TthHB8I Van91I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AfaI 501 GTAC GT^AC
AlwNI 604 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
BamHI 543 GGATCC G^GATCC
BbsI 487 GAAGAC GAAGAC(2/6)
BbvCI 234 GCTGAGG CCTCAGC(-5/-2)
BbvI 116 GCAGC GCAGC(8/12)
BclI 309 TGATCA T^GATCA
BglII 557 AGATCT A^GATCT
BlpI 96 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 96 GCTCAGC GC^T[ACGT]AGC
BpuAI 487 GAAGAC GAAGAC(2/6)
BsaI 403 GAGACC GGTCTC(1/5)
BsaWI 657 ACCGGA [AT]^CCGG[AT]
BseRI 121 CTCCTC GAGGAG(10/8)
BsmFI 81 GTCCC GGGAC(10/14)
BspHI 312 TCATGA T^CATGA
BsrFI 92 GCCGGC [GA]^CCGG[CT]
BssHII 112 GCGCGC G^CGCGC
BstF5I 61 CATCC GGATG(2/0)
BstXI 338 CCAGCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 96 GCTCAGC GC^T[ACGT]AGC
Cfr10I 92 GCCGGC [GA]^CCGG[CT]
DraII 605 AGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 301 GAAGAG CTCTTC(1/4)
EcoO109I 605 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 710 GAATTC G^AATTC
EcoRV 319 GATATC GAT^ATC
FbaI 309 TGATCA T^GATCA
FokI 61 CATCC GGATG(9/13)
HindIII 532 AAGCTT A^AGCTT
Ksp632I 301 GAAGAG CTCTTC(1/4)
KspI 44 CCGCGG CCGC^GG
MaeIII 721 GTGAC ^GT[ACGT]AC
MseI 370 TTAA T^TAA
NaeI 92 GCCGGC GCC^GGC
NgoMIV 92 GCCGGC G^CCGGC
PstI 85 CTGCAG CTGCA^G
RcaI 312 TCATGA T^CATGA
RsaI 501 GTAC GT^AC
SacII 44 CCGCGG CCGC^GG
SexAI 503 ACCAGGT A^CC[AT]GGT
SfcI 85 CTGCAG C^T[GA][CT]AG
StuI 350 AGGCCT AGG^CCT
Tru9I 370 TTAA T^TAA
TseI 116 GCAGC G^C[AT]GC
Tsp45I 721 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 45 CGCG CG^CG
113 CGCG
AlwI 544 GATCC GGATC(4/5)
543 GGATC
AvaII 141 GGTCC G^G[AT]CC
150 GGACC
BalI 164 TGGCCA TGG^CCA
182 TGGCCA
BfaI 277 CTAG C^TAG
565 CTAG
BsmAI 403 GAGAC GTCTC(1/5)
625 GAGAC
BsmI 525 GAATGC GAATGC(1/-1)
750 GAATGC
BspMI 39 ACCTGC ACCTGC(4/8)
230 ACCTGC
BstUI 45 CGCG CG^CG
113 CGCG
BstYI 543 GGATCC [GA]^GATC[CT]
557 AGATCT
CfoI 112 GCGC GCG^C
114 GCGC
DsaI 44 CCGCGG C^C[GA][CT]GG
179 CCGTGG
HapII 93 CCGG C^CGG
658 CCGG
HhaI 112 GCGC GCG^C
114 GCGC
HinP1I 112 GCGC G^CGC
114 GCGC
HpaII 93 CCGG C^CGG
658 CCGG
HphI 37 TCACC GGTGA(8/7)
216 TCACC
MaeI 277 CTAG C^TAG
565 CTAG
MflI 543 GGATCC [GA]^GATC[CT]
557 AGATCT
MluNI 164 TGGCCA TGG^CCA
182 TGGCCA
MscI 164 TGGCCA TGG^CCA
182 TGGCCA
MspA1I 44 CCGCGG C[AC]G^C[GT]G
65 CAGCGG
MspI 93 CCGG C^CGG
658 CCGG
MvnI 45 CGCG CG^CG
113 CGCG
MwoI 87 GCAGTGCCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
92 GCCGGCTCAGC
NlaIV 5 GGCCCC GG[ACGT]^[ACGT]CC
543 GGATCC
SfaNI 600 GATGC GCATC(5/9)
60 GCATC
XbaI 276 TCTAGA T^CTAGA
564 TCTAGA
XhoII 543 GGATCC [GA]^GATC[CT]
557 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AcsI AluI ApoI BpmI
BsaJI BsiYI BslI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EaeI
EcoRII Fnu4HI HaeIII ItaI MboI
MboII MnlI MvaI NdeII NlaIII
NspI Sau3AI Sau96I ScrFI Tsp509I
TspRI