Restriction map for
pF1KB9959
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BalI BamHI BbrPI BciVI
BclI BfrI BglII BlnI BsaAI
BsaBI BseAI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI Eam1105I Eco47III EcoNI EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HincII HindII HindIII HpaI
KspI MamI MfeI MluI MluNI
MroI MscI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tru9I Tsp509I Tth111I XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 850 GACGTC GACGT^C
Acc65I 815 GGTACC G^GTACC
AccII 1015 CGCG CG^CG
AlwNI 1165 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 896 CCTCAGG CC^T[ACGT]AGG
Asp718I 815 GGTACC G^GTACC
BbeI 1327 GGCGCC GGCGC^C
BbvCI 496 GCTGAGG CCTCAGC(-5/-2)
BcgI 41 CGACGCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1480 CTAG C^TAG
BlpI 1018 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1018 GCTGAGC GC^T[ACGT]AGC
BsaI 1042 GAGACC GGTCTC(1/5)
BsaWI 85 ACCGGA [AT]^CCGG[AT]
BsgI 984 CTGCAC GTGCAG(16/14)
BsiEI 822 CGGCCG CG[GA][CT]^CG
BsmBI 315 CGTCTC CGTCTC(1/5)
Bsp1407I 188 TGTACA T^GTACA
BsrBI 301 GAGCGG CCGCTC(-3/-3)
BsrDI 1107 CATTGC GCAATG(2/0)
BsrFI 1145 GCCGGC [GA]^CCGG[CT]
BsrGI 188 TGTACA T^GTACA
BstUI 1015 CGCG CG^CG
Bsu36I 896 CCTCAGG CC^T[ACGT]AGG
CelII 1018 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1145 GCCGGC [GA]^CCGG[CT]
DraIII 949 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 618 GACTCAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 822 CGGCCG C^GGCCG
EclXI 822 CGGCCG C^GGCCG
Eco52I 822 CGGCCG C^GGCCG
Eco81I 896 CCTCAGG CC^T[ACGT]AGG
KasI 1327 GGCGCC G^GCGCC
KpnI 815 GGTACC GGTAC^C
MaeI 1480 CTAG C^TAG
MvnI 1015 CGCG CG^CG
NaeI 1145 GCCGGC GCC^GGC
NarI 1327 GGCGCC GG^CGCC
NgoMIV 1145 GCCGGC G^CCGGC
PstI 1171 CTGCAG CTGCA^G
PvuII 937 CAGCTG CAG^CTG
SacI 355 GAGCTC GAGCT^C
SapI 1269 GCTCTTC GCTCTTC(1/4)
SfoI 1327 GGCGCC GGC^GCC
SmlI 1303 CTCAAG C^T[CT][GA]AG
SspBI 188 TGTACA T^GTACA
TfiI 1567 GATTC G^A[AT]TC
XbaI 1479 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwI 578 GGATC GGATC(4/5)
1476 GGATC
BbsI 1192 GAAGAC GAAGAC(2/6)
1462 GAAGAC
BglI 367 GCCACGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1440 GCCTCCAGGGC
BpuAI 1192 GAAGAC GAAGAC(2/6)
1462 GAAGAC
BstAPI 1164 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1344 GCAGGAAATGC
BstEII 201 GGTGACC G^GT[ACGT]ACC
705 GGTCACC
BstPI 201 GGTGACC G^GT[ACGT]ACC
705 GGTCACC
BstXI 995 CCAATGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1412 CCACAAGCCTGG
BstYI 578 GGATCT [GA]^GATC[CT]
1476 GGATCT
EarI 1270 CTCTTC CTCTTC(1/4)
1507 GAAGAG
EcoO65I 201 GGTGACC G^GT[ACGT]ACC
705 GGTCACC
HaeII 212 AGCGCC [GA]GCGC^[CT]
1327 GGCGCC
Ksp632I 1270 CTCTTC CTCTTC(1/4)
1507 GAAGAG
MflI 578 GGATCT [GA]^GATC[CT]
1476 GGATCT
PflMI 237 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
948 CCACGCAGTGG
PleI 618 GACTC GAGTC(4/5)
470 GAGTC
SfcI 1171 CTGCAG C^T[GA][CT]AG
1551 CTATAG
SmaI 5 CCCGGG CCC^GGG
1598 CCCGGG
Van91I 237 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
948 CCACGCAGTGG
XhoII 578 GGATCT [GA]^GATC[CT]
1476 GGATCT
XmaCI 5 CCCGGG C^CCGGG
1598 CCCGGG
XmaI 5 CCCGGG C^CCGGG
1598 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcyI AfaI AluI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TseI Tsp45I
TspRI TthHB8I