Restriction map for
pF1KB9972
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI BamHI
BbrPI BbsI BfrI BglII BpuAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BsrDI BssSI Bst1107I BstAPI
BstBI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoRI EcoRV
EcoT22I FseI HincII HindII HpaI
KpnI KspI MamI MfeI MflI
MluI MroI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SmlI SnaBI SpeI Sse8387I SspI
SwaI Tru9I Van91I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 1270 AAATTC [GA]^AATT[CT]
AcyI 60 GGCGCC G[GA]^CG[CT]C
ApaI 129 GGGCCC GGGCC^C
ApoI 1270 AAATTC [GA]^AATT[CT]
AspI 1657 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1865 TGCGCA TGC^GCA
AvrII 2056 CCTAGG C^CTAGG
BalI 1880 TGGCCA TGG^CCA
BbeI 60 GGCGCC GGCGC^C
BcgI 584 GCAAGATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 471 GGATAC GTATCC(6/5)
BclI 1265 TGATCA T^GATCA
BfaI 2057 CTAG C^TAG
BlnI 2056 CCTAGG C^CTAGG
BlpI 1993 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1993 GCTCAGC GC^T[ACGT]AGC
BsaAI 1030 TACGTG [CT]AC^GT[GA]
BsaHI 60 GGCGCC G[GA]^CG[CT]C
BsiEI 74 CGGCCG CG[GA][CT]^CG
Bsp1407I 1094 TGTACA T^GTACA
BsrGI 1094 TGTACA T^GTACA
BstXI 468 CCAGGATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1993 GCTCAGC GC^T[ACGT]AGC
EagI 74 CGGCCG C^GGCCG
EclXI 74 CGGCCG C^GGCCG
Eco52I 74 CGGCCG C^GGCCG
FbaI 1265 TGATCA T^GATCA
FspI 1865 TGCGCA TGC^GCA
Hin1I 60 GGCGCC G[GA]^CG[CT]C
KasI 60 GGCGCC G^GCGCC
MaeI 2057 CTAG C^TAG
MluNI 1880 TGGCCA TGG^CCA
MscI 1880 TGGCCA TGG^CCA
NarI 60 GGCGCC GG^CGCC
PflFI 1657 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1411 GAGTC GAGTC(4/5)
PpuMI 1699 AGGTCCC [GA]G^G[AT]CC[CT]
SapI 496 GAAGAGC GCTCTTC(1/4)
SfoI 60 GGCGCC GGC^GCC
SgrAI 62 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 1094 TGTACA T^GTACA
StuI 542 AGGCCT AGG^CCT
TaqI 217 TCGA T^CGA
Tth111I 1657 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 217 TCGA T^CGA
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 1668 GGATC GGATC(4/5)
2007 GGATC
AvaI 669 CCCGGG C^[CT]CG[GA]G
803 CCCGGG
BbvCI 1197 CCTCAGC CCTCAGC(-5/-2)
571 GCTGAGG
BglI 1140 GCCCAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1425 GCCCTCCGGGC
BsmBI 1651 GAGACG CGTCTC(1/5)
1732 GAGACG
BsoBI 669 CCCGGG C^[CT]CG[GA]G
803 CCCGGG
BsrBI 657 CCGCTC CCGCTC(-3/-3)
1006 CCGCTC
BsrFI 63 GCCGGC [GA]^CCGG[CT]
1474 GCCGGC
Cfr10I 63 GCCGGC [GA]^CCGG[CT]
1474 GCCGGC
EarI 1573 CTCTTC CTCTTC(1/4)
496 GAAGAG
HgaI 1000 GACGC GACGC(5/10)
381 GCGTC
HindIII 286 AAGCTT A^AGCTT
728 AAGCTT
Ksp632I 1573 CTCTTC CTCTTC(1/4)
496 GAAGAG
NaeI 63 GCCGGC GCC^GGC
1474 GCCGGC
NcoI 1823 CCATGG C^CATGG
2063 CCATGG
NgoMIV 63 GCCGGC G^CCGGC
1474 GCCGGC
NspI 1466 GCATGC [GA]CATG^[CT]
1868 GCATGC
SacI 1597 GAGCTC GAGCT^C
1741 GAGCTC
SmaI 669 CCCGGG CCC^GGG
803 CCCGGG
SphI 1466 GCATGC GCATG^C
1868 GCATGC
TfiI 426 GATTC G^A[AT]TC
1962 GAATC
Tsp509I 96 AATT ^AATT
1271 AATT
XmaCI 669 CCCGGG C^CCGGG
803 CCCGGG
XmaI 669 CCCGGG C^CCGGG
803 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssHII BssKI
BstEII BstF5I BstNI BstPI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
TspRI XcmI