Restriction map for
pF1KB9977
##################################
# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccIII AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanII BbrPI
BbvCI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsiYI BslI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Cac8I CelII Cfr10I ClaI CpoI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV FauI
FbaI FseI FspI HaeIII HapII
HpaI HpaII KpnI KspI MluI
MluNI MroI MscI MspA1I MspI
NaeI NciI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PstI
PvuI PvuII RcaI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 112 GTCGAC GT^[AC][GT]AC
AclI 886 AACGTT AA^CGTT
AcyI 48 GGCGCC G[GA]^CG[CT]C
AocI 138 CCTGAGG CC^T[ACGT]AGG
AvaII 385 GGTCC G^G[AT]CC
BanI 48 GGCGCC G^G[CT][GA]CC
BbeI 48 GGCGCC GGCGC^C
BcgI 26 CGACCCTAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 48 GGCGCC G[GA]^CG[CT]C
BseRI 95 CTCCTC GAGGAG(10/8)
BsmAI 583 GAGAC GTCTC(1/5)
BspMI 73 GCAGGT ACCTGC(4/8)
BstAPI 1336 GCATTATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1018 AGATCC [GA]^GATC[CT]
Bsu36I 138 CCTGAGG CC^T[ACGT]AGG
Cfr13I 385 GGTCC G^G[ACGT]CC
DraI 795 TTTAAA TTT^AAA
Eco81I 138 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1386 ATGCAT ATGCA^T
HaeII 48 GGCGCC [GA]GCGC^[CT]
HgaI 1267 GACGC GACGC(5/10)
Hin1I 48 GGCGCC G[GA]^CG[CT]C
HphI 577 TCACC GGTGA(8/7)
KasI 48 GGCGCC G^GCGCC
MfeI 1529 CAATTG C^AATTG
MflI 1018 AGATCC [GA]^GATC[CT]
MslI 1074 CACGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1529 CAATTG C^AATTG
MwoI 1336 GCATTATTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 48 GGCGCC GG^CGCC
NdeI 752 CATATG CA^TATG
NsiI 1386 ATGCAT ATGCA^T
Psp1406I 886 AACGTT AA^CGTT
SalI 112 GTCGAC G^TCGAC
Sau96I 385 GGTCC G^G[ACGT]CC
SfoI 48 GGCGCC GGC^GCC
SpeI 247 ACTAGT A^CTAGT
XhoII 1018 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 21 CGCG CG^CG
916 CGCG
AlwI 1019 GATCC GGATC(4/5)
1083 GATCC
Asp700I 1371 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1607 GAAATTATTC
BbsI 1375 GTCTTC GAAGAC(2/6)
1399 GTCTTC
BpmI 389 CTCCAG CTGGAG(16/14)
1001 CTCCAG
BpuAI 1375 GTCTTC GAAGAC(2/6)
1399 GTCTTC
BsaBI 429 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1561 GATGAAGATC
BstUI 21 CGCG CG^CG
916 CGCG
CfoI 20 GCGC GCG^C
49 GCGC
EarI 516 CTCTTC CTCTTC(1/4)
958 GAAGAG
EcoT14I 52 CCATGG C^C[AT][AT]GG
1229 CCATGG
HhaI 20 GCGC GCG^C
49 GCGC
HinP1I 20 GCGC G^CGC
49 GCGC
HincII 112 GTCGAC GT[CT]^[GA]AC
349 GTTGAC
HindII 112 GTCGAC GT[CT]^[GA]AC
349 GTTGAC
HindIII 483 AAGCTT A^AGCTT
986 AAGCTT
Ksp632I 516 CTCTTC CTCTTC(1/4)
958 GAAGAG
MaeII 622 ACGT A^CGT
887 ACGT
MamI 429 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1561 GATGAAGATC
MvnI 21 CGCG CG^CG
916 CGCG
NcoI 52 CCATGG C^CATGG
1229 CCATGG
NlaIV 48 GGCGCC GG[ACGT]^[ACGT]CC
782 GGCTCC
PleI 352 GACTC GAGTC(4/5)
160 GAGTC
SmlI 274 CTCAAG C^T[CT][GA]AG
1380 CTTGAG
SspI 241 AATATT AAT^ATT
544 AATATT
StyI 52 CCATGG C^C[AT][AT]GG
1229 CCATGG
Tsp45I 175 GTGAC ^GT[GC]AC
1265 GTGAC
XmnI 1371 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1607 GAAATTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI BbvI BfaI BmyI BsaJI
BsiHKAI Bsp1286I BsrI BssKI BstF5I
BstNI DdeI DpnII DsaI EcoRII
Fnu4HI FokI HinfI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MvaI NdeII NlaIII RsaI Sau3AI
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I