Restriction map for
pF1KE0012
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspHI AspI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BlnI
BpmI BsaAI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CfoI Cfr10I
ClaI CpoI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KspI MluI
MroI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 75 GGTACC G^GTACC
AciI 693 GCGG CCGC(-3/-1)
AflII 184 CTTAAG C^TTAAG
AflIII 927 ACATGT A^C[GA][CT]GT
AocI 1132 CCTGAGG CC^T[ACGT]AGG
Asp718I 75 GGTACC G^GTACC
BanI 75 GGTACC G^G[CT][GA]CC
BbsI 1097 GTCTTC GAAGAC(2/6)
BfaI 494 CTAG C^TAG
BfrI 184 CTTAAG C^TTAAG
BglII 1173 AGATCT A^GATCT
BlpI 660 GCTAAGC GC^T[ACGT]AGC
BmyI 913 GTGCCC G[AGT]GC[ACT]^C
Bpu1102I 660 GCTAAGC GC^T[ACGT]AGC
BpuAI 1097 GTCTTC GAAGAC(2/6)
BsaBI 1053 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 421 GAGGAG GAGGAG(10/8)
BsmAI 1253 GTCTC GTCTC(1/5)
BsmI 367 GAATGC GAATGC(1/-1)
Bsp1286I 913 GTGCCC G[AGT]GC[ACT]^C
BspHI 861 TCATGA T^CATGA
BspLU11I 927 ACATGT A^CATGT
BsrDI 540 CATTGC GCAATG(2/0)
Bsu36I 1132 CCTGAGG CC^T[ACGT]AGG
Cac8I 446 GCATGC GC[ACGT]^[ACGT]GC
CelII 660 GCTAAGC GC^T[ACGT]AGC
DraII 489 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 526 GACTCTTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1132 CCTGAGG CC^T[ACGT]AGG
EcoNI 946 CCTATTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 489 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 565 GATATC GAT^ATC
EcoT22I 219 ATGCAT ATGCA^T
FauI 693 GCGGG CCCGC(4/6)
KpnI 75 GGTACC GGTAC^C
MaeI 494 CTAG C^TAG
MaeII 8 ACGT A^CGT
MamI 1053 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1315 CAATTG C^AATTG
MspA1I 1190 CAGCTG C[AC]G^C[GT]G
MunI 1315 CAATTG C^AATTG
NsiI 219 ATGCAT ATGCA^T
PpuMI 489 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 1190 CAGCTG CAG^CTG
RcaI 861 TCATGA T^CATGA
SapI 1417 GCTCTTC GCTCTTC(1/4)
SfaNI 885 GATGC GCATC(5/9)
SfcI 822 CTACAG C^T[GA][CT]AG
SphI 446 GCATGC GCATG^C
TaqI 114 TCGA T^CGA
TthHB8I 114 TCGA T^CGA
XcmI 502 CCAAGCTATGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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Asp700I 1015 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1357 GAATTTGTTC
AvaII 490 GGTCC G^G[AT]CC
1480 GGACC
BalI 386 TGGCCA TGG^CCA
765 TGGCCA
BclI 324 TGATCA T^GATCA
591 TGATCA
BcnI 27 CCCGG CC^[GC]GG
59 CCGGG
BglI 371 GCCTGGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
915 GCCCATTGGGC
BspMI 396 ACCTGC ACCTGC(4/8)
155 GCAGGT
EaeI 386 TGGCCA [CT]^GGCC[GA]
765 TGGCCA
EarI 1075 CTCTTC CTCTTC(1/4)
1418 CTCTTC
EcoT14I 799 CCAAGG C^C[AT][AT]GG
845 CCAAGG
FbaI 324 TGATCA T^GATCA
591 TGATCA
HapII 28 CCGG C^CGG
59 CCGG
HpaII 28 CCGG C^CGG
59 CCGG
HphI 536 TCACC GGTGA(8/7)
1411 TCACC
Ksp632I 1075 CTCTTC CTCTTC(1/4)
1418 CTCTTC
MluNI 386 TGGCCA TGG^CCA
765 TGGCCA
MscI 386 TGGCCA TGG^CCA
765 TGGCCA
MspI 28 CCGG C^CGG
59 CCGG
MwoI 371 GCCTGGTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
915 GCCCATTGGGC
NciI 27 CCCGG CC^[GC]GG
59 CCGGG
NspI 446 GCATGC [GA]CATG^[CT]
927 ACATGT
SmlI 184 CTTAAG C^T[CT][GA]AG
937 CTCAAG
StyI 799 CCAAGG C^C[AT][AT]GG
845 CCAAGG
Tsp45I 466 GTGAC ^GT[GC]AC
535 GTCAC
TspRI 1068 CACTG CA[GC]TG[ACGT][ACGT]^
1484 CAGTG
XmnI 1015 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1357 GAATTTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BsaJI BsiYI BslI BsmFI
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII DraI EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI TfiI Tru9I TseI Tsp509I
XhoII