Restriction map for
pF1KE0029
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccII AciI
AclI AcyI AflIII AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BcgI
BciVI BclI BcnI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsiEI BsiWI BsmBI BsmI
BspHI BspLU11I BspMI BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MaeII MluI
MslI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccIII 1337 TCCGGA T^CCGGA
AgeI 527 ACCGGT A^CCGGT
AlwNI 1166 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 304 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1196 CTCGAG C^[CT]CG[GA]G
BanI 1308 GGTGCC G^G[CT][GA]CC
BbsI 1480 GAAGAC GAAGAC(2/6)
BpmI 157 CTGGAG CTGGAG(16/14)
BpuAI 1480 GAAGAC GAAGAC(2/6)
BseAI 1337 TCCGGA T^CCGGA
BsgI 518 CTGCAC GTGCAG(16/14)
BsoBI 1196 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1224 TGTACA T^GTACA
BspDI 1454 ATCGAT AT^CGAT
BspEI 1337 TCCGGA T^CCGGA
BsrGI 1224 TGTACA T^GTACA
BstAPI 228 GCAGTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 1454 ATCGAT AT^CGAT
DraII 758 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 1539 GACCAAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 762 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 758 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 43 GAATTC G^AATTC
EcoRV 784 GATATC GAT^ATC
HindIII 1119 AAGCTT A^AGCTT
HphI 71 TCACC GGTGA(8/7)
MaeIII 913 GTCAC ^GT[ACGT]AC
MroI 1337 TCCGGA T^CCGGA
MspA1I 175 CAGCTG C[AC]G^C[GT]G
NcoI 431 CCATGG C^CATGG
PaeR7I 1196 CTCGAG C^TCGAG
PinAI 527 ACCGGT A^CCGGT
PleI 1018 GAGTC GAGTC(4/5)
PpuMI 758 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 175 CAGCTG CAG^CTG
SspBI 1224 TGTACA T^GTACA
Tsp45I 913 GTCAC ^GT[GC]AC
XcmI 238 CCAGAATTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1196 CTCGAG C^TCGAG
XmnI 304 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AvaII 759 GGACC G^G[AT]CC
1088 GGACC
BalI 765 TGGCCA TGG^CCA
1299 TGGCCA
BamHI 427 GGATCC G^GATCC
696 GGATCC
BfaI 805 CTAG C^TAG
1489 CTAG
BsaBI 490 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1049 GATTTTCATC
BsiYI 762 CCTTGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1400 CCAGCATTGGG
BslI 762 CCTTGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1400 CCAGCATTGGG
BsmAI 278 GAGAC GTCTC(1/5)
1548 GTCTC
BsmFI 758 GGGAC GGGAC(10/14)
1087 GGGAC
BsrFI 527 ACCGGT [GA]^CCGG[CT]
1484 ACCGGC
BstYI 427 GGATCC [GA]^GATC[CT]
696 GGATCC
Cfr10I 527 ACCGGT [GA]^CCGG[CT]
1484 ACCGGC
Cfr13I 759 GGACC G^G[ACGT]CC
1088 GGACC
DsaI 431 CCATGG C^C[GA][CT]GG
646 CCACGG
EaeI 765 TGGCCA [CT]^GGCC[GA]
1299 TGGCCA
EarI 1104 CTCTTC CTCTTC(1/4)
983 GAAGAG
Ksp632I 1104 CTCTTC CTCTTC(1/4)
983 GAAGAG
MaeI 805 CTAG C^TAG
1489 CTAG
MamI 490 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1049 GATTTTCATC
MfeI 422 CAATTG C^AATTG
1082 CAATTG
MflI 427 GGATCC [GA]^GATC[CT]
696 GGATCC
MluNI 765 TGGCCA TGG^CCA
1299 TGGCCA
MscI 765 TGGCCA TGG^CCA
1299 TGGCCA
MunI 422 CAATTG C^AATTG
1082 CAATTG
PstI 226 CTGCAG CTGCA^G
1474 CTGCAG
Sau96I 759 GGACC G^G[ACGT]CC
1088 GGACC
SfaNI 865 GATGC GCATC(5/9)
1036 GATGC
SfcI 226 CTGCAG C^T[GA][CT]AG
1474 CTGCAG
XhoII 427 GGATCC [GA]^GATC[CT]
696 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AcsI AfaI AflII AluI AlwI
ApoI AspHI BanII BbvCI BbvI
BfrI BmyI BsaJI BsaWI BseRI
BsiHKAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MboI MboII
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV RsaI SacI
Sau3AI ScrFI SmlI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I