Restriction map for pF1KE0975
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AccI        AcsI        AflII       AgeI        AhdI        
Aor51HI     ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AviII       BamHI       
BbeI        BbsI        BcgI        BclI        BfrI        
BglII       BlpI        Bpu1102I    BpuAI       BsaBI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspHI       BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstYI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DraI        DrdI        DsaI        Eam1105I    Eco47III    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KasI        KspI        MamI        MfeI        
MflI        MluI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PinAI       PmeI        PshAI       PshBI       PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TfiI        Tsp509I     
Tth111I     XbaI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII         331  GACGTC               GACGT^C
Acc65I        545  GGTACC               G^GTACC
AclI         1225  AACGTT               AA^CGTT
AcyI          331  GACGTC               G[GA]^CG[CT]C
AflIII        599  ACATGT               A^C[GA][CT]GT
ApaI         1068  GGGCCC               GGGCC^C
Asp718I       545  GGTACC               G^GTACC
AvrII        1269  CCTAGG               C^CTAGG
BanII        1068  GGGCCC               G[GA]GC[CT]^C
BbrPI         337  CACGTG               CAC^GTG
BbvCI        1123  CCTCAGC              CCTCAGC(-5/-2)
BciVI         617  GTATCC               GTATCC(6/5)
BglI         1390  GCCAACCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         1269  CCTAGG               C^CTAGG
BsaHI         331  GACGTC               G[GA]^CG[CT]C
BsiEI        1491  CGGCCG               CG[GA][CT]^CG
BspLU11I      599  ACATGT               A^CATGT
BstAPI        393  GCACGTTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         192  CCAGGAGTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI         1491  CGGCCG               C^GGCCG
EarI          570  CTCTTC               CTCTTC(1/4)
EclXI        1491  CGGCCG               C^GGCCG
Eco52I       1491  CGGCCG               C^GGCCG
HaeII        1305  AGCGCC               [GA]GCGC^[CT]
Hin1I         331  GACGTC               G[GA]^CG[CT]C
KpnI          545  GGTACC               GGTAC^C
Ksp632I       570  CTCTTC               CTCTTC(1/4)
MseI          877  TTAA                 T^TAA
MslI            6  CACCGTAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI          599  ACATGT               [GA]CATG^[CT]
PaeR7I       1056  CTCGAG               C^TCGAG
PmaCI         337  CACGTG               CAC^GTG
PmlI          337  CACGTG               CAC^GTG
Psp1406I     1225  AACGTT               AA^CGTT
SexAI         266  ACCTGGT              A^CC[AT]GGT
SfiI         1389  GGCCAACCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          965  CCCGGG               CCC^GGG
SmlI         1056  CTCGAG               C^T[CT][GA]AG
StuI          827  AGGCCT               AGG^CCT
TaqI         1057  TCGA                 T^CGA
Tru9I         877  TTAA                 T^TAA
TthHB8I      1057  TCGA                 T^CGA
XcmI          422  CCAATGTGCTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1056  CTCGAG               C^TCGAG
XmaCI         965  CCCGGG               C^CCGGG
XmaI          965  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccII         882  CGCG                 CG^CG
             1012  CGCG                
Alw44I          3  GTGCAC               G^TGCAC
              400  GTGCAC              
ApaLI           3  GTGCAC               G^TGCAC
              400  GTGCAC              
BfaI         1136  CTAG                 C^TAG
             1270  CTAG                
BsaI          642  GAGACC               GGTCTC(1/5)
              694  GAGACC              
BseRI         187  CTCCTC               GAGGAG(10/8)
              715  GAGGAG              
BspMI        1100  ACCTGC               ACCTGC(4/8)
             1604  ACCTGC              
BsrBI         477  CCGCTC               CCGCTC(-3/-3)
             1244  CCGCTC              
BssSI         944  CACGAG               CACGAG(-5/-1)
             1201  CACGAG              
BstUI         882  CGCG                 CG^CG
             1012  CGCG                
DraIII        394  CACGTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1624  CACCATGTG           
HgaI          789  GACGC                GACGC(5/10)
             1013  GCGTC               
MaeI         1136  CTAG                 C^TAG
             1270  CTAG                
MboII         350  TCTTC                GAAGA(8/7)
              571  TCTTC               
MvnI          882  CGCG                 CG^CG
             1012  CGCG                
PflMI         536  CCACACGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              672  CCACCAGCTGG         
PstI          325  CTGCAG               CTGCA^G
              895  CTGCAG              
SfcI          325  CTGCAG               C^T[GA][CT]AG
              895  CTGCAG              
Van91I        536  CCACACGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              672  CCACCAGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AccIII      AciI        AfaI        AluI        AlwI        
AlwNI       AocI        AspHI       AvaI        AvaII       
BalI        BanI        BbvI        BcnI        BmyI        
BpmI        BsaAI       BsaJI       BsaWI       BseAI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspEI       BsrI        
BssKI       BstF5I      BstNI       Bsu36I      Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MluNI       MnlI        MroI        MscI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
StyI        TseI        Tsp45I      TspRI       
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