Restriction map for
pF1KB6436
##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII AgeI AhdI AlwI AlwNI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvrII
BalI BamHI BanII BbrPI BcgI
BciVI BclI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I CelII ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FauI FbaI FseI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MslI MunI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI TaqI Tsp45I Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 154 CGCG CG^CG
AfaI 881 GTAC GT^AC
AflII 283 CTTAAG C^TTAAG
Alw44I 416 GTGCAC G^TGCAC
AocI 511 CCTGAGG CC^T[ACGT]AGG
ApaLI 416 GTGCAC G^TGCAC
AvaII 435 GGACC G^G[AT]CC
AviII 65 TGCGCA TGC^GCA
BbeI 48 GGCGCC GGCGC^C
BbsI 850 GTCTTC GAAGAC(2/6)
BbvCI 784 CCTCAGC CCTCAGC(-5/-2)
BfaI 385 CTAG C^TAG
BfrI 283 CTTAAG C^TTAAG
BpuAI 850 GTCTTC GAAGAC(2/6)
BsmAI 275 GTCTC GTCTC(1/5)
BsmFI 399 GGGAC GGGAC(10/14)
BstUI 154 CGCG CG^CG
BstXI 17 CCAGGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 511 CCTGAGG CC^T[ACGT]AGG
DpnII 427 GATC ^GATC
DraI 631 TTTAAA TTT^AAA
DraII 324 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 587 CCATGG C^C[GA][CT]GG
EaeI 108 TGGCCG [CT]^GGCC[GA]
EarI 142 CTCTTC CTCTTC(1/4)
Eco81I 511 CCTGAGG CC^T[ACGT]AGG
EcoO109I 324 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 769 GAATTC G^AATTC
EcoT14I 587 CCATGG C^C[AT][AT]GG
FspI 65 TGCGCA TGC^GCA
HaeII 48 GGCGCC [GA]GCGC^[CT]
HgaI 118 GACGC GACGC(5/10)
KasI 48 GGCGCC G^GCGCC
Ksp632I 142 CTCTTC CTCTTC(1/4)
MaeI 385 CTAG C^TAG
MaeII 334 ACGT A^CGT
MaeIII 919 GTAAC ^GT[ACGT]AC
MboI 427 GATC ^GATC
MvnI 154 CGCG CG^CG
NarI 48 GGCGCC GG^CGCC
NcoI 587 CCATGG C^CATGG
NdeI 224 CATATG CA^TATG
NdeII 427 GATC ^GATC
PleI 43 GAGTC GAGTC(4/5)
RsaI 881 GTAC GT^AC
Sau3AI 427 GATC ^GATC
SfoI 48 GGCGCC GGC^GCC
SgrAI 50 CGCCGGCG C[GA]^CCGG[CT]G
StuI 19 AGGCCT AGG^CCT
StyI 587 CCATGG C^C[AT][AT]GG
TspRI 269 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 48 GGCGCC G[GA]^CG[CT]C
118 GACGCC
Asp700I 658 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
709 GAAAATATTC
BanI 48 GGCGCC G^G[CT][GA]CC
751 GGTGCC
BcnI 11 CCGGG CC^[GC]GG
114 CCCGG
BsaHI 48 GGCGCC G[GA]^CG[CT]C
118 GACGCC
BsrFI 51 GCCGGC [GA]^CCGG[CT]
172 GCCGGC
BstF5I 840 CATCC GGATG(2/0)
915 GGATG
Cfr10I 51 GCCGGC [GA]^CCGG[CT]
172 GCCGGC
FokI 840 CATCC GGATG(9/13)
915 GGATG
Hin1I 48 GGCGCC G[GA]^CG[CT]C
118 GACGCC
MseI 284 TTAA T^TAA
632 TTAA
MspA1I 100 CCGCTG C[AC]G^C[GT]G
129 CCGCTG
NaeI 51 GCCGGC GCC^GGC
172 GCCGGC
NciI 11 CCGGG CC^[GC]GG
114 CCCGG
NgoMIV 51 GCCGGC G^CCGGC
172 GCCGGC
SspI 712 AATATT AAT^ATT
762 AATATT
Tru9I 284 TTAA T^TAA
632 TTAA
XcmI 279 CCAGCTTAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
755 CCAAGAGAATATTGG
XmnI 658 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
709 GAAAATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AcsI AluI ApoI AspHI
BbvI BglI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BsrI
BssKI BstNI Cac8I CfoI Cfr13I
DdeI EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MboII MnlI MspI MvaI
MwoI NlaIII NlaIV PstI Sau96I
ScrFI SfaNI SfcI SmlI TfiI
TseI Tsp509I