Restriction map for
pF1KE0057
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BcgI
BfrI BlnI BsaAI BsaHI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I Bsu36I ClaI CpoI
DraI DraIII Eam1105I Eco81I EcoRV
EcoT22I FseI FspI Hin1I HincII
HindII HpaI KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 23 enzymes) #
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ApaI 2602 GGGCCC GGGCC^C
Asp700I 387 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI 1456 GGATAC GTATCC(6/5)
BclI 624 TGATCA T^GATCA
BglI 3445 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 925 GTGCAG GTGCAG(16/14)
BsrFI 530 ACCGGC [GA]^CCGG[CT]
BstEII 1410 GGTGACC G^GT[ACGT]ACC
BstPI 1410 GGTGACC G^GT[ACGT]ACC
Cfr10I 530 ACCGGC [GA]^CCGG[CT]
DrdI 2828 GACCACTAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1410 GGTGACC G^GT[ACGT]ACC
EcoRI 1285 GAATTC G^AATTC
FbaI 624 TGATCA T^GATCA
HgaI 3321 GCGTC GACGC(5/10)
MaeII 1508 ACGT A^CGT
NdeI 1024 CATATG CA^TATG
SacI 1981 GAGCTC GAGCT^C
SapI 3234 GAAGAGC GCTCTTC(1/4)
SexAI 2468 ACCAGGT A^CC[AT]GGT
SpeI 832 ACTAGT A^CTAGT
SspI 132 AATATT AAT^ATT
XmnI 387 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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Aor51HI 1760 AGCGCT AGC^GCT
1826 AGCGCT
AvaI 938 CTCGGG C^[CT]CG[GA]G
1978 CCCGAG
BamHI 481 GGATCC G^GATCC
2452 GGATCC
BbvCI 1528 CCTCAGC CCTCAGC(-5/-2)
1619 CCTCAGC
BsaBI 1668 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2447 GATTGGGATC
BsaI 2826 GAGACC GGTCTC(1/5)
2850 GGTCTC
BsiEI 1733 CGGCCG CG[GA][CT]^CG
2841 CGGCCG
BsoBI 938 CTCGGG C^[CT]CG[GA]G
1978 CCCGAG
BsrBI 19 GAGCGG CCGCTC(-3/-3)
2077 GAGCGG
BstXI 1775 CCATTATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2243 CCAACCTCCTGG
EagI 1733 CGGCCG C^GGCCG
2841 CGGCCG
EclXI 1733 CGGCCG C^GGCCG
2841 CGGCCG
Eco47III 1760 AGCGCT AGC^GCT
1826 AGCGCT
Eco52I 1733 CGGCCG C^GGCCG
2841 CGGCCG
MamI 1668 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2447 GATTGGGATC
MseI 2162 TTAA T^TAA
3286 TTAA
MslI 562 CACCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2274 CACTGCCATG
NcoI 2123 CCATGG C^CATGG
2747 CCATGG
PflMI 2935 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3318 CCAGCGTCTGG
TaqI 380 TCGA T^CGA
708 TCGA
Tru9I 2162 TTAA T^TAA
3286 TTAA
TthHB8I 380 TCGA T^CGA
708 TCGA
Van91I 2935 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3318 CCAGCGTCTGG
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TfiI TseI Tsp45I Tsp509I
TspRI XhoII