Restriction map for
pF1KE0059
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BlnI BsaAI BsaBI
BsaHI BsaI BseAI BsgI BsiEI
BsiWI BsiYI BslI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CfoI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoT14I EcoT22I
FauI FbaI Fnu4HI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI ItaI KasI KpnI Ksp632I
KspI MaeI MaeII MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI MwoI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PleI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StyI
SwaI TseI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AfaI 339 GTAC GT^AC
AflIII 633 ACATGT A^C[GA][CT]GT
AgeI 745 ACCGGT A^CCGGT
AseI 487 ATTAAT AT^TAAT
AsnI 487 ATTAAT AT^TAAT
AvaII 69 GGACC G^G[AT]CC
BbsI 496 GAAGAC GAAGAC(2/6)
BbvCI 4 GCTGAGG CCTCAGC(-5/-2)
BglII 231 AGATCT A^GATCT
BlpI 1049 GCTCAGC GC^T[ACGT]AGC
BpmI 342 CTCCAG CTGGAG(16/14)
Bpu1102I 1049 GCTCAGC GC^T[ACGT]AGC
BpuAI 496 GAAGAC GAAGAC(2/6)
BsaJI 190 CCCTGG C^C[ACGT][ACGT]GG
BsaWI 745 ACCGGT [AT]^CCGG[AT]
BseRI 52 GAGGAG GAGGAG(10/8)
BspDI 240 ATCGAT AT^CGAT
BspLU11I 633 ACATGT A^CATGT
BsrFI 745 ACCGGT [GA]^CCGG[CT]
BstF5I 194 GGATG GGATG(2/0)
Cac8I 897 GCATGC GC[ACGT]^[ACGT]GC
CelII 1049 GCTCAGC GC^T[ACGT]AGC
Cfr10I 745 ACCGGT [GA]^CCGG[CT]
Cfr13I 69 GGACC G^G[ACGT]CC
ClaI 240 ATCGAT AT^CGAT
EcoRV 395 GATATC GAT^ATC
FokI 194 GGATG GGATG(9/13)
HapII 746 CCGG C^CGG
HincII 1145 GTCAAC GT[CT]^[GA]AC
HindII 1145 GTCAAC GT[CT]^[GA]AC
HindIII 970 AAGCTT A^AGCTT
HpaII 746 CCGG C^CGG
MaeIII 637 GTTAC ^GT[ACGT]AC
MboII 496 GAAGA GAAGA(8/7)
MspI 746 CCGG C^CGG
NdeI 1262 CATATG CA^TATG
NlaIV 69 GGACCC GG[ACGT]^[ACGT]CC
PinAI 745 ACCGGT A^CCGGT
PshBI 487 ATTAAT AT^TAAT
RsaI 339 GTAC GT^AC
Sau96I 69 GGACC G^G[ACGT]CC
ScaI 338 AGTACT AGT^ACT
SphI 897 GCATGC GCATG^C
SspI 423 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwI 10 GGATC GGATC(4/5)
1389 GGATC
BssKI 66 CCTGG ^CC[ACGT]GG
191 CCTGG
BstNI 66 CCTGG CC^[AT]GG
191 CCTGG
BstYI 231 AGATCT [GA]^GATC[CT]
1389 GGATCT
EcoRII 66 CCTGG ^CC[AT]GG
191 CCTGG
HinfI 238 GAATC G^A[ACGT]TC
737 GAATC
MflI 231 AGATCT [GA]^GATC[CT]
1389 GGATCT
MseI 488 TTAA T^TAA
1292 TTAA
MvaI 66 CCTGG CC^[AT]GG
191 CCTGG
ScrFI 66 CCTGG CC^[ACGT]GG
191 CCTGG
SfaNI 645 GCATC GCATC(5/9)
1233 GCATC
SmlI 155 CTCAAG C^T[CT][GA]AG
334 CTTGAG
TaqI 241 TCGA T^CGA
1314 TCGA
TfiI 238 GAATC G^A[AT]TC
737 GAATC
Tru9I 488 TTAA T^TAA
1292 TTAA
TthHB8I 241 TCGA T^CGA
1314 TCGA
XhoII 231 AGATCT [GA]^GATC[CT]
1389 GGATCT
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AcsI AluI ApoI AspHI BanII
BmyI BsiHKAI BsmFI Bsp1286I BsrI
DdeI DpnII EcoRI HaeIII HphI
MboI MnlI MslI NdeII NlaIII
NspI SacI Sau3AI StuI Tsp509I
TspRI