Restriction map for
pF1KE0067
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# NO CUT ENZYMES ( 69 enzymes) #
##################################
AccIII AclI AflII AflIII AhdI
AocI Aor51HI AscI AseI AsnI
AspEI AspI AviII AvrII BbrPI
BbvCI BcgI BfrI BlnI BseAI
BsiWI BspDI BspEI BspLU11I BsrBI
Bsu36I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoT22I FseI FspI HincII HindII
HpaI MluI MroI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SalI SfiI SgrAI SnaBI SpeI
SphI SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 2622 GACGTC GACGT^C
AgeI 88 ACCGGT A^CCGGT
Alw44I 1235 GTGCAC G^TGCAC
ApaI 4111 GGGCCC GGGCC^C
ApaLI 1235 GTGCAC G^TGCAC
BamHI 3461 GGATCC G^GATCC
BbeI 1346 GGCGCC GGCGC^C
BciVI 1157 GGATAC GTATCC(6/5)
BglI 3685 GCCCCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2514 AGATCT A^GATCT
BsaAI 2814 CACGTA [CT]AC^GT[GA]
BsaBI 2473 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 284 GGTCTC GGTCTC(1/5)
BsiEI 195 CGACCG CG[GA][CT]^CG
BsrDI 587 CATTGC GCAATG(2/0)
BssHII 71 GCGCGC G^CGCGC
BssSI 2879 CTCGTG CACGAG(-5/-1)
BstAPI 394 GCAAAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3059 TTCGAA TT^CGAA
BstEII 3309 GGTCACC G^GT[ACGT]ACC
BstPI 3309 GGTCACC G^GT[ACGT]ACC
DraI 999 TTTAAA TTT^AAA
EcoNI 1936 CCTCCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3309 GGTCACC G^GT[ACGT]ACC
EcoRV 3812 GATATC GAT^ATC
KasI 1346 GGCGCC G^GCGCC
KspI 185 CCGCGG CCGC^GG
MamI 2473 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1346 GGCGCC GG^CGCC
NspI 2805 ACATGC [GA]CATG^[CT]
NspV 3059 TTCGAA TT^CGAA
PflMI 2783 CCAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 88 ACCGGT A^CCGGT
PshAI 3157 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 185 CCGCGG CCGC^GG
SapI 3371 GCTCTTC GCTCTTC(1/4)
SbfI 3517 CCTGCAGG CCTGCA^GG
ScaI 2785 AGTACT AGT^ACT
SfoI 1346 GGCGCC GGC^GCC
SfuI 3059 TTCGAA TT^CGAA
SmaI 3689 CCCGGG CCC^GGG
Sse8387I 3517 CCTGCAGG CCTGCA^GG
Van91I 2783 CCAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2157 TCTAGA T^CTAGA
XmaCI 3689 CCCGGG C^CCGGG
XmaI 3689 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 39 enzymes) #
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Acc65I 27 GGTACC G^GTACC
2039 GGTACC
AccI 921 GTATAC GT^[AC][GT]AC
2130 GTATAC
AccII 72 CGCG CG^CG
186 CGCG
Asp700I 1764 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2410 GAAGGAGTTC
Asp718I 27 GGTACC G^GTACC
2039 GGTACC
AvaI 560 CCCGAG C^[CT]CG[GA]G
3689 CCCGGG
BalI 1556 TGGCCA TGG^CCA
3187 TGGCCA
BbsI 2692 GAAGAC GAAGAC(2/6)
1784 GTCTTC
BlpI 635 GCTCAGC GC^T[ACGT]AGC
4331 GCTCAGC
Bpu1102I 635 GCTCAGC GC^T[ACGT]AGC
4331 GCTCAGC
BpuAI 2692 GAAGAC GAAGAC(2/6)
1784 GTCTTC
BsaWI 88 ACCGGT [AT]^CCGG[AT]
2648 TCCGGT
BsmI 233 GCATTC GAATGC(1/-1)
3957 GCATTC
BsoBI 560 CCCGAG C^[CT]CG[GA]G
3689 CCCGGG
BsrFI 88 ACCGGT [GA]^CCGG[CT]
1966 ACCGGC
Bst1107I 921 GTATAC GTA^TAC
2130 GTATAC
BstUI 72 CGCG CG^CG
186 CGCG
BstZ17I 921 GTATAC GTA^TAC
2130 GTATAC
CelII 635 GCTCAGC GC^T[ACGT]AGC
4331 GCTCAGC
Cfr10I 88 ACCGGT [GA]^CCGG[CT]
1966 ACCGGC
DrdI 2596 GACAACTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2848 GACTTGACAGTC
EaeI 1556 TGGCCA [CT]^GGCC[GA]
3187 TGGCCA
EcoRI 2749 GAATTC G^AATTC
3062 GAATTC
HaeII 1346 GGCGCC [GA]GCGC^[CT]
3745 AGCGCC
HgaI 3368 GACGC GACGC(5/10)
1820 GCGTC
HindIII 104 AAGCTT A^AGCTT
2390 AAGCTT
KpnI 27 GGTACC GGTAC^C
2039 GGTACC
MfeI 2972 CAATTG C^AATTG
3236 CAATTG
MluNI 1556 TGGCCA TGG^CCA
3187 TGGCCA
MscI 1556 TGGCCA TGG^CCA
3187 TGGCCA
MunI 2972 CAATTG C^AATTG
3236 CAATTG
MvnI 72 CGCG CG^CG
186 CGCG
NcoI 991 CCATGG C^CATGG
3710 CCATGG
PpuMI 1447 GGGTCCC [GA]G^G[AT]CC[CT]
3437 AGGACCT
SacI 970 GAGCTC GAGCT^C
3642 GAGCTC
SexAI 814 ACCTGGT A^CC[AT]GGT
2440 ACCAGGT
SspI 952 AATATT AAT^ATT
3490 AATATT
StuI 1758 AGGCCT AGG^CCT
2724 AGGCCT
XmnI 1764 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2410 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BanII BbvI BclI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I Bsp1407I
BspHI BspMI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII