Restriction map for
pF1KE0071
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI Bst1107I
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI MamI
MfeI MluI MroI MunI NdeI
NheI NruI NsiI NspI PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SbfI SexAI SfiI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 265 GTCGAC GT^[AC][GT]AC
AlwI 1032 GGATC GGATC(4/5)
AocI 2009 CCTCAGG CC^T[ACGT]AGG
BbeI 295 GGCGCC GGCGC^C
BbvCI 1710 GCTGAGG CCTCAGC(-5/-2)
BciVI 770 GTATCC GTATCC(6/5)
BfaI 1606 CTAG C^TAG
BpmI 57 CTGGAG CTGGAG(16/14)
BsaI 1762 GAGACC GGTCTC(1/5)
BsiEI 498 CGGCCG CG[GA][CT]^CG
BsmBI 1756 GAGACG CGTCTC(1/5)
BsmI 724 GAATGC GAATGC(1/-1)
BssHII 14 GCGCGC G^CGCGC
BstBI 73 TTCGAA TT^CGAA
BstYI 1032 GGATCT [GA]^GATC[CT]
Bsu36I 2009 CCTCAGG CC^T[ACGT]AGG
DraIII 1986 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 498 CGGCCG C^GGCCG
EarI 1230 CTCTTC CTCTTC(1/4)
EclXI 498 CGGCCG C^GGCCG
Eco52I 498 CGGCCG C^GGCCG
Eco81I 2009 CCTCAGG CC^T[ACGT]AGG
EcoNI 1926 CCTCGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 792 CCATGG C^C[AT][AT]GG
KasI 295 GGCGCC G^GCGCC
Ksp632I 1230 CTCTTC CTCTTC(1/4)
KspI 135 CCGCGG CCGC^GG
MaeI 1606 CTAG C^TAG
MflI 1032 GGATCT [GA]^GATC[CT]
MseI 1127 TTAA T^TAA
NarI 295 GGCGCC GG^CGCC
NcoI 792 CCATGG C^CATGG
NotI 497 GCGGCCGC GC^GGCCGC
NspV 73 TTCGAA TT^CGAA
PaeR7I 1036 CTCGAG C^TCGAG
PvuII 1167 CAGCTG CAG^CTG
SacI 60 GAGCTC GAGCT^C
SacII 135 CCGCGG CCGC^GG
SalI 265 GTCGAC G^TCGAC
SapI 1229 GCTCTTC GCTCTTC(1/4)
ScaI 515 AGTACT AGT^ACT
SfoI 295 GGCGCC GGC^GCC
SfuI 73 TTCGAA TT^CGAA
StyI 792 CCATGG C^C[AT][AT]GG
TfiI 388 GATTC G^A[AT]TC
Tru9I 1127 TTAA T^TAA
XhoI 1036 CTCGAG C^TCGAG
XhoII 1032 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcyI 262 GGCGTC G[GA]^CG[CT]C
295 GGCGCC
ApaI 652 GGGCCC GGGCC^C
1575 GGGCCC
Asp700I 1386 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1887 GAATGGCTTC
BalI 440 TGGCCA TGG^CCA
1778 TGGCCA
BcgI 671 CGATCTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1028 GCACGGATCTCG
BsaHI 262 GGCGTC G[GA]^CG[CT]C
295 GGCGCC
BsrI 1548 CCAGT ACTGG(1/-1)
2112 CCAGT
BssSI 1489 CACGAG CACGAG(-5/-1)
1927 CTCGTG
BstXI 1253 CCAAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1624 CCATTCCCCTGG
DrdI 1801 GACATCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1951 GACACCGCCGTC
Hin1I 262 GGCGTC G[GA]^CG[CT]C
295 GGCGCC
HincII 97 GTCAAC GT[CT]^[GA]AC
265 GTCGAC
HindII 97 GTCAAC GT[CT]^[GA]AC
265 GTCGAC
MaeII 1328 ACGT A^CGT
2123 ACGT
MluNI 440 TGGCCA TGG^CCA
1778 TGGCCA
MscI 440 TGGCCA TGG^CCA
1778 TGGCCA
NaeI 947 GCCGGC GCC^GGC
1297 GCCGGC
NgoMIV 947 GCCGGC G^CCGGC
1297 GCCGGC
SmaI 581 CCCGGG CCC^GGG
1572 CCCGGG
Tsp509I 1091 AATT ^AATT
1215 AATT
XmaCI 581 CCCGGG C^CCGGG
1572 CCCGGG
XmaI 581 CCCGGG C^CCGGG
1572 CCCGGG
XmnI 1386 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1887 GAATGGCTTC
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BglI BmyI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BssKI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TseI Tsp45I TspRI TthHB8I XcmI