Restriction map for
pF1KE0076
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# NO CUT ENZYMES ( 78 enzymes) #
##################################
AccIII AclI AcsI AflII AflIII
AhdI Aor51HI ApoI AscI AseI
AsnI Asp700I AspEI AviII BamHI
BbrPI BcgI BciVI BclI BfrI
BglII BsaAI BseAI BsiWI BsmI
BspDI BspEI BspLU11I BsrDI BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI Eam1105I Eco47III EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KspI MfeI MluI MroI
MseI MunI NdeI NruI NsiI
NspV PacI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SexAI SfuI SnaBI
SpeI SphI SspI SwaI Tru9I
Tsp509I XbaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 2191 GTAGAC GT^[AC][GT]AC
AgeI 297 ACCGGT A^CCGGT
AspI 4638 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 610 CCTAGG C^CTAGG
BbeI 215 GGCGCC GGCGC^C
BlnI 610 CCTAGG C^CTAGG
BsaBI 372 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 297 ACCGGT [AT]^CCGG[AT]
Bsp1407I 426 TGTACA T^GTACA
BspHI 380 TCATGA T^CATGA
BsrGI 426 TGTACA T^GTACA
BssHII 2749 GCGCGC G^CGCGC
BstEII 1132 GGTCACC G^GT[ACGT]ACC
BstPI 1132 GGTCACC G^GT[ACGT]ACC
EcoO65I 1132 GGTCACC G^GT[ACGT]ACC
HincII 3904 GTCAAC GT[CT]^[GA]AC
HindII 3904 GTCAAC GT[CT]^[GA]AC
KasI 215 GGCGCC G^GCGCC
MamI 372 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 215 GGCGCC GG^CGCC
NheI 4516 GCTAGC G^CTAGC
NotI 1640 GCGGCCGC GC^GGCCGC
NspI 3965 GCATGT [GA]CATG^[CT]
PaeR7I 790 CTCGAG C^TCGAG
PflFI 4638 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 297 ACCGGT A^CCGGT
RcaI 380 TCATGA T^CATGA
SacI 2479 GAGCTC GAGCT^C
SapI 4228 GAAGAGC GCTCTTC(1/4)
SbfI 4674 CCTGCAGG CCTGCA^GG
SfoI 215 GGCGCC GGC^GCC
SgrAI 1183 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 4674 CCTGCAGG CCTGCA^GG
SspBI 426 TGTACA T^GTACA
Tth111I 4638 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 790 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AatII 1024 GACGTC GACGT^C
1840 GACGTC
Alw44I 1936 GTGCAC G^TGCAC
2459 GTGCAC
ApaLI 1936 GTGCAC G^TGCAC
2459 GTGCAC
BbsI 1485 GTCTTC GAAGAC(2/6)
1597 GTCTTC
BbvCI 817 CCTCAGC CCTCAGC(-5/-2)
2852 CCTCAGC
BpuAI 1485 GTCTTC GAAGAC(2/6)
1597 GTCTTC
BsmBI 305 GAGACG CGTCTC(1/5)
2223 GAGACG
BstYI 1172 AGATCC [GA]^GATC[CT]
1439 AGATCC
DrdI 3397 GACTGCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3468 GACGGCTGTGTC
EagI 1641 CGGCCG C^GGCCG
1703 CGGCCG
EarI 2503 CTCTTC CTCTTC(1/4)
4228 GAAGAG
EclXI 1641 CGGCCG C^GGCCG
1703 CGGCCG
Eco52I 1641 CGGCCG C^GGCCG
1703 CGGCCG
Ksp632I 2503 CTCTTC CTCTTC(1/4)
4228 GAAGAG
MflI 1172 AGATCC [GA]^GATC[CT]
1439 AGATCC
ScaI 77 AGTACT AGT^ACT
1832 AGTACT
XhoII 1172 AGATCC [GA]^GATC[CT]
1439 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
Asp718I AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KpnI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SmaI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XmaCI XmaI