Restriction map for
pF1KE0077
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BciVI BclI BfrI BglII
BlnI BsaWI BseAI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BssHII
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraI DrdI Eam1105I EcoRI
EcoT22I FbaI HincII HindII HindIII
HpaI KpnI KspI MfeI MluI
MroI MseI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I RcaI RsrII SacII SalI
SbfI ScaI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tru9I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 2260 CGCG CG^CG
AcsI 865 AAATTC [GA]^AATT[CT]
AocI 1313 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2282 AGCGCT AGC^GCT
ApoI 865 AAATTC [GA]^AATT[CT]
AspI 3590 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2800 TGCGCA TGC^GCA
BbeI 1667 GGCGCC GGCGC^C
BbrPI 3103 CACGTG CAC^GTG
BlpI 2704 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2704 GCTCAGC GC^T[ACGT]AGC
BsaBI 1728 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2483 GAGACG CGTCTC(1/5)
Bsp1407I 3368 TGTACA T^GTACA
BsrBI 520 GAGCGG CCGCTC(-3/-3)
BsrGI 3368 TGTACA T^GTACA
BstUI 2260 CGCG CG^CG
Bsu36I 1313 CCTCAGG CC^T[ACGT]AGG
CelII 2704 GCTCAGC GC^T[ACGT]AGC
EagI 618 CGGCCG C^GGCCG
EclXI 618 CGGCCG C^GGCCG
Eco47III 2282 AGCGCT AGC^GCT
Eco52I 618 CGGCCG C^GGCCG
Eco81I 1313 CCTCAGG CC^T[ACGT]AGG
EcoRV 628 GATATC GAT^ATC
FseI 2808 GGCCGGCC GGCCGG^CC
FspI 2800 TGCGCA TGC^GCA
HgaI 2485 GACGC GACGC(5/10)
KasI 1667 GGCGCC G^GCGCC
MamI 1728 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2260 CGCG CG^CG
NaeI 2809 GCCGGC GCC^GGC
NarI 1667 GGCGCC GG^CGCC
NgoMIV 2809 GCCGGC G^CCGGC
PflFI 3590 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3103 CACGTG CAC^GTG
PmlI 3103 CACGTG CAC^GTG
PvuI 7 CGATCG CGAT^CG
SfoI 1667 GGCGCC GGC^GCC
SmaI 2789 CCCGGG CCC^GGG
SspBI 3368 TGTACA T^GTACA
Tth111I 3590 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2789 CCCGGG C^CCGGG
XmaI 2789 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 1667 GGCGCC G[GA]^CG[CT]C
2485 GACGCC
Alw44I 2824 GTGCAC G^TGCAC
2919 GTGCAC
ApaLI 2824 GTGCAC G^TGCAC
2919 GTGCAC
BamHI 149 GGATCC G^GATCC
648 GGATCC
BbsI 2754 GAAGAC GAAGAC(2/6)
1633 GTCTTC
BbvCI 1647 CCTCAGC CCTCAGC(-5/-2)
930 GCTGAGG
BcgI 1496 GCACCAGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2921 GCACTTTCCTCG
BpuAI 2754 GAAGAC GAAGAC(2/6)
1633 GTCTTC
BsaAI 739 TACGTG [CT]AC^GT[GA]
3103 CACGTG
BsaHI 1667 GGCGCC G[GA]^CG[CT]C
2485 GACGCC
BsaI 911 GAGACC GGTCTC(1/5)
214 GGTCTC
BsiEI 7 CGATCG CG[GA][CT]^CG
618 CGGCCG
BspMI 611 ACCTGC ACCTGC(4/8)
3143 ACCTGC
BsrFI 2316 ACCGGC [GA]^CCGG[CT]
2809 GCCGGC
BssSI 829 CACGAG CACGAG(-5/-1)
3310 CTCGTG
BstEII 1839 GGTCACC G^GT[ACGT]ACC
2223 GGTGACC
BstPI 1839 GGTCACC G^GT[ACGT]ACC
2223 GGTGACC
Cfr10I 2316 ACCGGC [GA]^CCGG[CT]
2809 GCCGGC
DraIII 782 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
1864 CACCTGGTG
EcoNI 1737 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2205 CCTGTACGAGG
EcoO65I 1839 GGTCACC G^GT[ACGT]ACC
2223 GGTGACC
Hin1I 1667 GGCGCC G[GA]^CG[CT]C
2485 GACGCC
NcoI 849 CCATGG C^CATGG
3303 CCATGG
PpuMI 675 GGGTCCC [GA]G^G[AT]CC[CT]
1928 GGGACCT
SacI 1900 GAGCTC GAGCT^C
2560 GAGCTC
SexAI 1865 ACCTGGT A^CC[AT]GGT
3591 ACCAGGT
SfiI 192 GGCCTTTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1086 GGCCCAGGAGGCC
StuI 734 AGGCCT AGG^CCT
1317 AGGCCT
Tsp509I 866 AATT ^AATT
887 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII