Restriction map for
pF1KE0086
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
Eam1105I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI HindIII
HpaI KpnI MaeI MamI MfeI
MluI MroI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NruI
NsiI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 355 GTGCAC G^TGCAC
AlwNI 889 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 355 GTGCAC G^TGCAC
AspHI 355 GTGCAC G[AT]GC[AT]^C
AviII 1448 TGCGCA TGC^GCA
BamHI 1228 GGATCC G^GATCC
BbeI 1480 GGCGCC GGCGC^C
BglI 100 GCCACCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiHKAI 355 GTGCAC G[AT]GC[AT]^C
BsmAI 562 GAGAC GTCTC(1/5)
BsmBI 562 GAGACG CGTCTC(1/5)
BspHI 962 TCATGA T^CATGA
BsrDI 622 CATTGC GCAATG(2/0)
BstAPI 888 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 1458 GACACACGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1446 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1448 TGCGCA TGC^GCA
KasI 1480 GGCGCC G^GCGCC
NarI 1480 GGCGCC GG^CGCC
NotI 121 GCGGCCGC GC^GGCCGC
PaeR7I 613 CTCGAG C^TCGAG
PpuMI 987 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 962 TCATGA T^CATGA
SfoI 1480 GGCGCC GGC^GCC
StuI 1443 AGGCCT AGG^CCT
XhoI 613 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 279 GTAC GT^AC
597 GTAC
Aor51HI 314 AGCGCT AGC^GCT
609 AGCGCT
BalI 461 TGGCCA TGG^CCA
710 TGGCCA
BlpI 378 GCTCAGC GC^T[ACGT]AGC
1331 GCTCAGC
BpmI 724 CTGGAG CTGGAG(16/14)
808 CTGGAG
Bpu1102I 378 GCTCAGC GC^T[ACGT]AGC
1331 GCTCAGC
BseRI 232 GAGGAG GAGGAG(10/8)
973 GAGGAG
BsiEI 122 CGGCCG CG[GA][CT]^CG
1511 CGGCCG
BsrFI 1082 ACCGGC [GA]^CCGG[CT]
1365 GCCGGT
BssSI 1064 CACGAG CACGAG(-5/-1)
1462 CACGAG
BstYI 1228 GGATCC [GA]^GATC[CT]
1550 AGATCC
CelII 378 GCTCAGC GC^T[ACGT]AGC
1331 GCTCAGC
Cfr10I 1082 ACCGGC [GA]^CCGG[CT]
1365 GCCGGT
DpnII 1229 GATC ^GATC
1551 GATC
EagI 122 CGGCCG C^GGCCG
1511 CGGCCG
EclXI 122 CGGCCG C^GGCCG
1511 CGGCCG
Eco47III 314 AGCGCT AGC^GCT
609 AGCGCT
Eco52I 122 CGGCCG C^GGCCG
1511 CGGCCG
KspI 212 CCGCGG CCGC^GG
761 CCGCGG
MboI 1229 GATC ^GATC
1551 GATC
MflI 1228 GGATCC [GA]^GATC[CT]
1550 AGATCC
MluNI 461 TGGCCA TGG^CCA
710 TGGCCA
MscI 461 TGGCCA TGG^CCA
710 TGGCCA
NdeII 1229 GATC ^GATC
1551 GATC
PflMI 621 CCATTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1131 CCAGCAGGTGG
RsaI 279 GTAC GT^AC
597 GTAC
SacII 212 CCGCGG CCGC^GG
761 CCGCGG
Sau3AI 1229 GATC ^GATC
1551 GATC
Van91I 621 CCATTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1131 CCAGCAGGTGG
XcmI 713 CCAGCCTGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1058 CCAAGCCACGAGTGG
XhoII 1228 GGATCC [GA]^GATC[CT]
1550 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
ApaI AvaI AvaII BanI BanII
BbvI BcnI BmyI BsaHI BsaJI
BsgI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII DsaI EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PleI PstI
PvuII Sau96I ScrFI SfaNI SfcI
SmaI SmlI TaqI TseI Tsp45I
TspRI TthHB8I XmaCI XmaI