Restriction map for
pF1KE0087
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BfrI BglI BglII
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MamI MluI MluNI MroI
MscI MspA1I MvnI NaeI NarI
NcoI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tth111I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AciI 185 CCGC CCGC(-3/-1)
AvaII 1070 GGACC G^G[AT]CC
BamHI 214 GGATCC G^GATCC
BanII 1787 GAGCTC G[GA]GC[CT]^C
BbvI 1257 GCTGC GCAGC(8/12)
BciVI 1173 GGATAC GTATCC(6/5)
BclI 2216 TGATCA T^GATCA
BlpI 1538 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1538 GCTCAGC GC^T[ACGT]AGC
BsaI 831 GGTCTC GGTCTC(1/5)
BsgI 108 GTGCAG GTGCAG(16/14)
BsmFI 1033 GGGAC GGGAC(10/14)
BsmI 892 GCATTC GAATGC(1/-1)
BspLU11I 122 ACATGT A^CATGT
BstYI 214 GGATCC [GA]^GATC[CT]
CelII 1538 GCTCAGC GC^T[ACGT]AGC
Cfr13I 1070 GGACC G^G[ACGT]CC
EarI 1790 CTCTTC CTCTTC(1/4)
EcoRI 1366 GAATTC G^AATTC
EcoT14I 1585 CCTTGG C^C[AT][AT]GG
EcoT22I 2747 ATGCAT ATGCA^T
FauI 184 CCCGC CCCGC(4/6)
FbaI 2216 TGATCA T^GATCA
Fnu4HI 1257 GCTGC GC^[ACGT]GC
HincII 876 GTTGAC GT[CT]^[GA]AC
HindII 876 GTTGAC GT[CT]^[GA]AC
HindIII 1508 AAGCTT A^AGCTT
ItaI 1257 GCTGC GC^[ACGT]GC
Ksp632I 1790 CTCTTC CTCTTC(1/4)
MflI 214 GGATCC [GA]^GATC[CT]
NdeI 188 CATATG CA^TATG
NheI 2136 GCTAGC G^CTAGC
NsiI 2747 ATGCAT ATGCA^T
PflMI 851 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 2386 GACTC GAGTC(4/5)
SacI 1787 GAGCTC GAGCT^C
SapI 1789 GCTCTTC GCTCTTC(1/4)
Sau96I 1070 GGACC G^G[ACGT]CC
ScaI 2449 AGTACT AGT^ACT
StyI 1585 CCTTGG C^C[AT][AT]GG
TseI 1257 GCTGC G^C[AT]GC
Tsp45I 1729 GTGAC ^GT[GC]AC
Van91I 851 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 214 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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BcnI 135 CCCGG CC^[GC]GG
732 CCGGG
BpmI 722 CTGGAG CTGGAG(16/14)
2085 CTGGAG
BseRI 2388 CTCCTC GAGGAG(10/8)
97 GAGGAG
Bsp1407I 438 TGTACA T^GTACA
1700 TGTACA
BsrGI 438 TGTACA T^GTACA
1700 TGTACA
BstF5I 78 GGATG GGATG(2/0)
1977 GGATG
FokI 78 GGATG GGATG(9/13)
1977 GGATG
HapII 136 CCGG C^CGG
732 CCGG
HpaII 136 CCGG C^CGG
732 CCGG
MfeI 868 CAATTG C^AATTG
2207 CAATTG
MspI 136 CCGG C^CGG
732 CCGG
MunI 868 CAATTG C^AATTG
2207 CAATTG
NciI 135 CCCGG CC^[GC]GG
732 CCGGG
PshAI 825 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2205 GACAATTGTC
SphI 2441 GCATGC GCATG^C
2489 GCATGC
SspBI 438 TGTACA T^GTACA
1700 TGTACA
XbaI 1476 TCTAGA T^CTAGA
2350 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI AspHI BbsI BfaI BmyI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrDI BsrI BssKI
BstNI Cac8I DdeI DpnII DraI
EcoRII EcoRV HaeIII HinfI HphI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI ScrFI SfaNI SfcI TaqI
TfiI Tru9I Tsp509I TspRI TthHB8I